Trace elements species fractionation in rye flour and rye (Secale cereale L.) seedlings
The fractionation of Cd, Cu, Mo, Ni, and Zn species in extracts of rye (cv. Fernando) seedlings (grown up in both standard and Cd2+-enriched medium) and rye flour was performed by SEC/ICP-MS method. The majority of Cu, Zn, and Ni in all samples were bound in the 1-2 kDa fraction. Molybdenum occurred...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2009-02-01
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| Series: | Czech Journal of Food Sciences |
| Subjects: | |
| Online Access: | https://cjfs.agriculturejournals.cz/artkey/cjf-200901-0005_trace-elements-species-fractionation-in-rye-flour-and-rye-secale-cereale-l-seedlings.php |
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| author | Jan Polák Oto Mestek Richard Koplík Jiří Šantrůček Jana Komínková Milan Kodíček |
| author_facet | Jan Polák Oto Mestek Richard Koplík Jiří Šantrůček Jana Komínková Milan Kodíček |
| author_sort | Jan Polák |
| collection | DOAJ |
| description | The fractionation of Cd, Cu, Mo, Ni, and Zn species in extracts of rye (cv. Fernando) seedlings (grown up in both standard and Cd2+-enriched medium) and rye flour was performed by SEC/ICP-MS method. The majority of Cu, Zn, and Ni in all samples were bound in the 1-2 kDa fraction. Molybdenum occurred in all samples in the fraction of 3 kDa. During five days of cultivation in a solution of 30 µmol/l Cd2+, the plants accumulated as much as 5 mg/kg fresh matter of Cd, but its soluble portion represented only 12-15%. The prevailing portion of Cd complexes was contained in the fraction of 3 kDa, while the minor part occurred in the fraction of 20 kDa. The speciation of elevated Cd in plants differs from that of other metals present at a physiological level. The metal-rich fractions of the extracts of all samples (i.e. those of 1-2 kDa) were refined by immobilised metal affinity chromatography. The isolated ligands of trace elements were peptides rich in dicarboxylic aminoacids. |
| first_indexed | 2024-04-10T08:35:57Z |
| format | Article |
| id | doaj.art-71ec81ae8bf14ca2be1dbb6172de981e |
| institution | Directory Open Access Journal |
| issn | 1212-1800 1805-9317 |
| language | English |
| last_indexed | 2024-04-10T08:35:57Z |
| publishDate | 2009-02-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Food Sciences |
| spelling | doaj.art-71ec81ae8bf14ca2be1dbb6172de981e2023-02-23T03:27:30ZengCzech Academy of Agricultural SciencesCzech Journal of Food Sciences1212-18001805-93172009-02-01271394810.17221/33/2008-CJFScjf-200901-0005Trace elements species fractionation in rye flour and rye (Secale cereale L.) seedlingsJan Polák0Oto Mestek1Richard Koplík2Jiří ŠantrůčekJana Komínková3Milan KodíčekDepartment of Analytical Chemistry, Faculty of Engineering,Department of Analytical Chemistry, Faculty of Engineering,Department of Food Chemistry and Analysis andDepartment of Analytical Chemistry, Faculty of Engineering,The fractionation of Cd, Cu, Mo, Ni, and Zn species in extracts of rye (cv. Fernando) seedlings (grown up in both standard and Cd2+-enriched medium) and rye flour was performed by SEC/ICP-MS method. The majority of Cu, Zn, and Ni in all samples were bound in the 1-2 kDa fraction. Molybdenum occurred in all samples in the fraction of 3 kDa. During five days of cultivation in a solution of 30 µmol/l Cd2+, the plants accumulated as much as 5 mg/kg fresh matter of Cd, but its soluble portion represented only 12-15%. The prevailing portion of Cd complexes was contained in the fraction of 3 kDa, while the minor part occurred in the fraction of 20 kDa. The speciation of elevated Cd in plants differs from that of other metals present at a physiological level. The metal-rich fractions of the extracts of all samples (i.e. those of 1-2 kDa) were refined by immobilised metal affinity chromatography. The isolated ligands of trace elements were peptides rich in dicarboxylic aminoacids.https://cjfs.agriculturejournals.cz/artkey/cjf-200901-0005_trace-elements-species-fractionation-in-rye-flour-and-rye-secale-cereale-l-seedlings.phpryemetalspeciationtrace elementsmaldi-msicp-msimac |
| spellingShingle | Jan Polák Oto Mestek Richard Koplík Jiří Šantrůček Jana Komínková Milan Kodíček Trace elements species fractionation in rye flour and rye (Secale cereale L.) seedlings Czech Journal of Food Sciences rye metal speciation trace elements maldi-ms icp-ms imac |
| title | Trace elements species fractionation in rye flour and rye (Secale cereale L.) seedlings |
| title_full | Trace elements species fractionation in rye flour and rye (Secale cereale L.) seedlings |
| title_fullStr | Trace elements species fractionation in rye flour and rye (Secale cereale L.) seedlings |
| title_full_unstemmed | Trace elements species fractionation in rye flour and rye (Secale cereale L.) seedlings |
| title_short | Trace elements species fractionation in rye flour and rye (Secale cereale L.) seedlings |
| title_sort | trace elements species fractionation in rye flour and rye secale cereale l seedlings |
| topic | rye metal speciation trace elements maldi-ms icp-ms imac |
| url | https://cjfs.agriculturejournals.cz/artkey/cjf-200901-0005_trace-elements-species-fractionation-in-rye-flour-and-rye-secale-cereale-l-seedlings.php |
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