Differentially expressed whey proteins of donkey and bovine colostrum revealed with a label-free proteomics approach
This study aimed to analyze and compare the differentially expressed whey proteins (DEWPs) of donkey and bovine colostrum using high-performance liquid chromatography with tandem mass spectrometry-based proteomics. A total of 620 and 696 whey proteins were characterized in the donkey and bovine colo...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2023-07-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453022002233 |
| _version_ | 1797705327078014976 |
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| author | Mohan Li Qilong Li Haikun Yu Xiumin Zhang Dehao Li Wanying Song Yan Zheng Xiqing Yue |
| author_facet | Mohan Li Qilong Li Haikun Yu Xiumin Zhang Dehao Li Wanying Song Yan Zheng Xiqing Yue |
| author_sort | Mohan Li |
| collection | DOAJ |
| description | This study aimed to analyze and compare the differentially expressed whey proteins (DEWPs) of donkey and bovine colostrum using high-performance liquid chromatography with tandem mass spectrometry-based proteomics. A total of 620 and 696 whey proteins were characterized in the donkey and bovine colostrum, respectively, including 383 common whey proteins. Among these common proteins, 80 were identified as DEWPs, including 21 upregulated and 59 downregulated DEWPs in donkey colostrum compared to bovine colostrum. Gene Ontology analysis revealed that these DEWPs were mainly related to cellular components, such as extracellular exosome, plasma membrane, and mitochondrion; biological processes, such as oxidation-reduction process, cell-cell adhesion, and small guanosine triphosphate (GTP) ase-mediated signal transduction; and molecular functions, such as GTP binding, GTPase activity, and soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein receptor activity. Metabolic pathway analysis suggested that the majority of the DEWPs were associated with soluble NSF factor attachment protein receptor interactions in vesicular transport, fatty acid biosynthesis, and estrogen signaling pathways. Our results provide a vital insight into the differences between donkey and bovine colostrum, along with important information on the significant components as nutritional and functional factors to be included in infant formula based on multiple milk sources. |
| first_indexed | 2024-03-12T05:35:30Z |
| format | Article |
| id | doaj.art-71ef0deb131848ecbf9eaecf81981df1 |
| institution | Directory Open Access Journal |
| issn | 2213-4530 |
| language | English |
| last_indexed | 2024-03-12T05:35:30Z |
| publishDate | 2023-07-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj.art-71ef0deb131848ecbf9eaecf81981df12023-09-03T06:30:54ZengTsinghua University PressFood Science and Human Wellness2213-45302023-07-0112412241231Differentially expressed whey proteins of donkey and bovine colostrum revealed with a label-free proteomics approachMohan Li0Qilong Li1Haikun Yu2Xiumin Zhang3Dehao Li4Wanying Song5Yan Zheng6Xiqing Yue7College of Food Science, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang 110866, ChinaBeijing Academy of Food Sciences, Beijing 100068, ChinaThe Fourth Affiliated Hospital of China Medical University, Shenyang 110866, ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang 110866, China; Corresponding authors at: College of Food Science, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang 110866, China; Corresponding authors at: College of Food Science, Shenyang Agricultural University, Shenyang 110866, ChinaThis study aimed to analyze and compare the differentially expressed whey proteins (DEWPs) of donkey and bovine colostrum using high-performance liquid chromatography with tandem mass spectrometry-based proteomics. A total of 620 and 696 whey proteins were characterized in the donkey and bovine colostrum, respectively, including 383 common whey proteins. Among these common proteins, 80 were identified as DEWPs, including 21 upregulated and 59 downregulated DEWPs in donkey colostrum compared to bovine colostrum. Gene Ontology analysis revealed that these DEWPs were mainly related to cellular components, such as extracellular exosome, plasma membrane, and mitochondrion; biological processes, such as oxidation-reduction process, cell-cell adhesion, and small guanosine triphosphate (GTP) ase-mediated signal transduction; and molecular functions, such as GTP binding, GTPase activity, and soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein receptor activity. Metabolic pathway analysis suggested that the majority of the DEWPs were associated with soluble NSF factor attachment protein receptor interactions in vesicular transport, fatty acid biosynthesis, and estrogen signaling pathways. Our results provide a vital insight into the differences between donkey and bovine colostrum, along with important information on the significant components as nutritional and functional factors to be included in infant formula based on multiple milk sources.http://www.sciencedirect.com/science/article/pii/S2213453022002233Bovine colostrumDonkey colostrumProteomicsWhey proteinGene OntologyKyoto Encyclopedia of Genes and Genomes |
| spellingShingle | Mohan Li Qilong Li Haikun Yu Xiumin Zhang Dehao Li Wanying Song Yan Zheng Xiqing Yue Differentially expressed whey proteins of donkey and bovine colostrum revealed with a label-free proteomics approach Food Science and Human Wellness Bovine colostrum Donkey colostrum Proteomics Whey protein Gene Ontology Kyoto Encyclopedia of Genes and Genomes |
| title | Differentially expressed whey proteins of donkey and bovine colostrum revealed with a label-free proteomics approach |
| title_full | Differentially expressed whey proteins of donkey and bovine colostrum revealed with a label-free proteomics approach |
| title_fullStr | Differentially expressed whey proteins of donkey and bovine colostrum revealed with a label-free proteomics approach |
| title_full_unstemmed | Differentially expressed whey proteins of donkey and bovine colostrum revealed with a label-free proteomics approach |
| title_short | Differentially expressed whey proteins of donkey and bovine colostrum revealed with a label-free proteomics approach |
| title_sort | differentially expressed whey proteins of donkey and bovine colostrum revealed with a label free proteomics approach |
| topic | Bovine colostrum Donkey colostrum Proteomics Whey protein Gene Ontology Kyoto Encyclopedia of Genes and Genomes |
| url | http://www.sciencedirect.com/science/article/pii/S2213453022002233 |
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