Biogenic Synthesis and Characterization of Chitosan-CuO Nanocomposite and Evaluation of Antibacterial Activity against Gram-Positive and -Negative Bacteria
Chitosan-copper oxide (CHT-CuO) nanocomposite was synthesized using olive leaf extract (OLE) as reducing agent and CuSO<sub>4</sub>⋅5H<sub>2</sub>O as precursor. CHT-CuO nanocomposite was prepared using an in situ method in which OLE was added to a solution of chitosan and Cu...
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MDPI AG
2022-04-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/14/9/1832 |
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| author | Peace Saviour Umoren Doga Kavaz Alexis Nzila Saravanan Sankaran Sankaran Saviour A. Umoren |
| author_facet | Peace Saviour Umoren Doga Kavaz Alexis Nzila Saravanan Sankaran Sankaran Saviour A. Umoren |
| author_sort | Peace Saviour Umoren |
| collection | DOAJ |
| description | Chitosan-copper oxide (CHT-CuO) nanocomposite was synthesized using olive leaf extract (OLE) as reducing agent and CuSO<sub>4</sub>⋅5H<sub>2</sub>O as precursor. CHT-CuO nanocomposite was prepared using an in situ method in which OLE was added to a solution of chitosan and CuSO<sub>4</sub>⋅5H<sub>2</sub>O mixture in the ratio of 1:5 (<i>v</i>/<i>v</i>) and heated at a temperature of 90 °C. The obtained CHT-CuO nanocomposite was characterized using field emission scanning electron microscopy (FE-SEM), X-ray diffraction (XRD), ultraviolet-visible (UV-Vis) spectrophotometry, energy-dispersive X-ray spectroscopy (EDAX), Fourier transform infrared spectroscopy (FTIR), and high-resolution transmission electron microscopy (TEM). TEM results indicated that CHT-CuO nanocomposite are spherical in shape with size ranging from 3.5 to 6.0 nm. Antibacterial activity of the synthesized nanocomposites was evaluated against Gram-positive (<i>Bacillus cereus</i>, <i>Staphyloccous haemolytica</i> and <i>Micrococcus Luteus</i>) and Gram-negative (<i>Escherichia coli</i>, <i>Pseudomonas citronellolis</i>, <i>Pseudomonas aeruginosa</i>, <i>kliebisella</i> sp., <i>Bradyrhizobium japonicum</i> and <i>Ralstonia pickettii</i>) species by cup platting or disc diffusion method. Overall, against all tested bacterial strains, the diameters of the inhibition zone of the three nanocomposites fell between 6 and 24 mm, and the order of the antimicrobial activity was as follows: CuO-1.0 > CuO-0.5 > CuO-2.0. The reference antibiotic amoxicillin and ciprofloxacin showed greater activity based on the diameter of zones of inhibition (between 15–32 mm) except for <i>S. heamolytica</i> and <i>P. citronellolis</i> bacteria strains. The nanocomposites MIC/MBC were between 0.1 and 0.01% against all tested bacteria, except <i>S. heamolityca</i> (>0.1%). Based on MIC/MBC values, CuO-0.5 and CuO-1.0 were more active than CuO-2.0, in line with the observations from the disc diffusion experiment. The findings indicate that these nanocomposites are efficacious against bacteria; however, Gram-positive bacteria were less susceptible. The synthesized CHT-CuO nanocomposite shows promising antimicrobial activities and could be utilized as an antibacterial agent in packaging and medical applications. |
| first_indexed | 2024-03-10T03:46:55Z |
| format | Article |
| id | doaj.art-71ef72eb8b31458cb9c81fe5ee72458f |
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| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-10T03:46:55Z |
| publishDate | 2022-04-01 |
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| series | Polymers |
| spelling | doaj.art-71ef72eb8b31458cb9c81fe5ee72458f2023-11-23T09:06:59ZengMDPI AGPolymers2073-43602022-04-01149183210.3390/polym14091832Biogenic Synthesis and Characterization of Chitosan-CuO Nanocomposite and Evaluation of Antibacterial Activity against Gram-Positive and -Negative BacteriaPeace Saviour Umoren0Doga Kavaz1Alexis Nzila2Saravanan Sankaran Sankaran3Saviour A. Umoren4Department of Bioengineering, Cyprus International University, via Mersin 10, Nicosia 98258, TurkeyDepartment of Bioengineering, Cyprus International University, via Mersin 10, Nicosia 98258, TurkeyDepartment of Bioengineering, King Fahd University of Petroleum and Minerals (KFUPM), Dhahran 31261, Saudi ArabiaDepartment of Bioengineering, King Fahd University of Petroleum and Minerals (KFUPM), Dhahran 31261, Saudi ArabiaInterdisciplinary Research Center for Advanced Materials, King Fahd University of Petroleum and Minerals (KFUPM), Dhahran 31261, Saudi ArabiaChitosan-copper oxide (CHT-CuO) nanocomposite was synthesized using olive leaf extract (OLE) as reducing agent and CuSO<sub>4</sub>⋅5H<sub>2</sub>O as precursor. CHT-CuO nanocomposite was prepared using an in situ method in which OLE was added to a solution of chitosan and CuSO<sub>4</sub>⋅5H<sub>2</sub>O mixture in the ratio of 1:5 (<i>v</i>/<i>v</i>) and heated at a temperature of 90 °C. The obtained CHT-CuO nanocomposite was characterized using field emission scanning electron microscopy (FE-SEM), X-ray diffraction (XRD), ultraviolet-visible (UV-Vis) spectrophotometry, energy-dispersive X-ray spectroscopy (EDAX), Fourier transform infrared spectroscopy (FTIR), and high-resolution transmission electron microscopy (TEM). TEM results indicated that CHT-CuO nanocomposite are spherical in shape with size ranging from 3.5 to 6.0 nm. Antibacterial activity of the synthesized nanocomposites was evaluated against Gram-positive (<i>Bacillus cereus</i>, <i>Staphyloccous haemolytica</i> and <i>Micrococcus Luteus</i>) and Gram-negative (<i>Escherichia coli</i>, <i>Pseudomonas citronellolis</i>, <i>Pseudomonas aeruginosa</i>, <i>kliebisella</i> sp., <i>Bradyrhizobium japonicum</i> and <i>Ralstonia pickettii</i>) species by cup platting or disc diffusion method. Overall, against all tested bacterial strains, the diameters of the inhibition zone of the three nanocomposites fell between 6 and 24 mm, and the order of the antimicrobial activity was as follows: CuO-1.0 > CuO-0.5 > CuO-2.0. The reference antibiotic amoxicillin and ciprofloxacin showed greater activity based on the diameter of zones of inhibition (between 15–32 mm) except for <i>S. heamolytica</i> and <i>P. citronellolis</i> bacteria strains. The nanocomposites MIC/MBC were between 0.1 and 0.01% against all tested bacteria, except <i>S. heamolityca</i> (>0.1%). Based on MIC/MBC values, CuO-0.5 and CuO-1.0 were more active than CuO-2.0, in line with the observations from the disc diffusion experiment. The findings indicate that these nanocomposites are efficacious against bacteria; however, Gram-positive bacteria were less susceptible. The synthesized CHT-CuO nanocomposite shows promising antimicrobial activities and could be utilized as an antibacterial agent in packaging and medical applications.https://www.mdpi.com/2073-4360/14/9/1832chitosancopper oxideolive leaf extractnanocompositeantibacterial activity |
| spellingShingle | Peace Saviour Umoren Doga Kavaz Alexis Nzila Saravanan Sankaran Sankaran Saviour A. Umoren Biogenic Synthesis and Characterization of Chitosan-CuO Nanocomposite and Evaluation of Antibacterial Activity against Gram-Positive and -Negative Bacteria Polymers chitosan copper oxide olive leaf extract nanocomposite antibacterial activity |
| title | Biogenic Synthesis and Characterization of Chitosan-CuO Nanocomposite and Evaluation of Antibacterial Activity against Gram-Positive and -Negative Bacteria |
| title_full | Biogenic Synthesis and Characterization of Chitosan-CuO Nanocomposite and Evaluation of Antibacterial Activity against Gram-Positive and -Negative Bacteria |
| title_fullStr | Biogenic Synthesis and Characterization of Chitosan-CuO Nanocomposite and Evaluation of Antibacterial Activity against Gram-Positive and -Negative Bacteria |
| title_full_unstemmed | Biogenic Synthesis and Characterization of Chitosan-CuO Nanocomposite and Evaluation of Antibacterial Activity against Gram-Positive and -Negative Bacteria |
| title_short | Biogenic Synthesis and Characterization of Chitosan-CuO Nanocomposite and Evaluation of Antibacterial Activity against Gram-Positive and -Negative Bacteria |
| title_sort | biogenic synthesis and characterization of chitosan cuo nanocomposite and evaluation of antibacterial activity against gram positive and negative bacteria |
| topic | chitosan copper oxide olive leaf extract nanocomposite antibacterial activity |
| url | https://www.mdpi.com/2073-4360/14/9/1832 |
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