Potential Antioxidative and Anti-Hyperuricemic Components Targeting Superoxide Dismutase and Xanthine Oxidase Explored from <i>Polygonatum Sibiricum</i> Red.
<i>Polygonatum sibiricum</i> Red. (<i>P. sibiricum</i>) has been used as a traditional Chinese medicine with a wide range of pharmacology effects. However, the responsible bioactive compounds and their mechanisms of action concerning its antioxidative and anti-hyperuricemic a...
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MDPI AG
2022-08-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/9/1651 |
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| author | Jing Li Zhi Wang Minxia Fan Guangwan Hu Mingquan Guo |
| author_facet | Jing Li Zhi Wang Minxia Fan Guangwan Hu Mingquan Guo |
| author_sort | Jing Li |
| collection | DOAJ |
| description | <i>Polygonatum sibiricum</i> Red. (<i>P. sibiricum</i>) has been used as a traditional Chinese medicine with a wide range of pharmacology effects. However, the responsible bioactive compounds and their mechanisms of action concerning its antioxidative and anti-hyperuricemic activities remain unexplored. In this work, the antioxidant capacity of <i>P. sibiricum</i> was firstly evaluated with the 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2’-azinobis-(3ethylbenzthiazoline)-6-sulfonic acid (ABTS) and ferric-reducing antioxidant power (FRAP) assays, from which the ethyl acetate (EA) fraction exhibited the highest DPPH, ABTS radical scavenging, and ferric-reducing capacities. Meanwhile, the EA fraction displayed the highest total phenolic and flavonoid contents among the four fractions. Next, the potential ligands from the EA fraction were screened out by bio-affinity ultrafiltration liquid chromatography-mass spectrometry (UF-LC-MS) with superoxide dismutase (SOD) and xanthine oxidase (XOD). As a result, <i>N</i>-<i>trans</i>-<i>p</i>-coumaroyloctopamine, <i>N</i>-<i>trans</i>-feruloyloctopamine, <i>N</i>-<i>trans</i>-feruloyltyramine were identified as potential SOD ligands, while <i>N</i>-<i>cis</i>-<i>p</i>-coumaroyltyramine was determined as potential XOD ligand. Additionally, these four ligands effectively interact with SOD and XOD in the molecular docking analysis, with binding energies (BEs) ranging from –6.83 to –6.51 kcal/mol, and the inhibition constants (Ki) from 9.83 to 16.83 μM, which were better than the positive controls. In conclusion, our results indicated that <i>P. sibiricum</i> has good antioxidative and anti-hyperuricemic activities, and its corresponding active ligands targeting SOD and XOD could be explored by the UF-LC-MS method. |
| first_indexed | 2024-03-10T00:53:51Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T00:53:51Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
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| series | Antioxidants |
| spelling | doaj.art-71f06921be294e74946d4882c8376bfa2023-11-23T14:46:39ZengMDPI AGAntioxidants2076-39212022-08-01119165110.3390/antiox11091651Potential Antioxidative and Anti-Hyperuricemic Components Targeting Superoxide Dismutase and Xanthine Oxidase Explored from <i>Polygonatum Sibiricum</i> Red.Jing Li0Zhi Wang1Minxia Fan2Guangwan Hu3Mingquan Guo4Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, ChinaCollege of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, ChinaKey Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, ChinaKey Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, ChinaKey Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China<i>Polygonatum sibiricum</i> Red. (<i>P. sibiricum</i>) has been used as a traditional Chinese medicine with a wide range of pharmacology effects. However, the responsible bioactive compounds and their mechanisms of action concerning its antioxidative and anti-hyperuricemic activities remain unexplored. In this work, the antioxidant capacity of <i>P. sibiricum</i> was firstly evaluated with the 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2’-azinobis-(3ethylbenzthiazoline)-6-sulfonic acid (ABTS) and ferric-reducing antioxidant power (FRAP) assays, from which the ethyl acetate (EA) fraction exhibited the highest DPPH, ABTS radical scavenging, and ferric-reducing capacities. Meanwhile, the EA fraction displayed the highest total phenolic and flavonoid contents among the four fractions. Next, the potential ligands from the EA fraction were screened out by bio-affinity ultrafiltration liquid chromatography-mass spectrometry (UF-LC-MS) with superoxide dismutase (SOD) and xanthine oxidase (XOD). As a result, <i>N</i>-<i>trans</i>-<i>p</i>-coumaroyloctopamine, <i>N</i>-<i>trans</i>-feruloyloctopamine, <i>N</i>-<i>trans</i>-feruloyltyramine were identified as potential SOD ligands, while <i>N</i>-<i>cis</i>-<i>p</i>-coumaroyltyramine was determined as potential XOD ligand. Additionally, these four ligands effectively interact with SOD and XOD in the molecular docking analysis, with binding energies (BEs) ranging from –6.83 to –6.51 kcal/mol, and the inhibition constants (Ki) from 9.83 to 16.83 μM, which were better than the positive controls. In conclusion, our results indicated that <i>P. sibiricum</i> has good antioxidative and anti-hyperuricemic activities, and its corresponding active ligands targeting SOD and XOD could be explored by the UF-LC-MS method.https://www.mdpi.com/2076-3921/11/9/1651<i>Polygonatum sibiricum</i>UF-LC-MSantioxidativeanti-hyperuricemicSODXOD |
| spellingShingle | Jing Li Zhi Wang Minxia Fan Guangwan Hu Mingquan Guo Potential Antioxidative and Anti-Hyperuricemic Components Targeting Superoxide Dismutase and Xanthine Oxidase Explored from <i>Polygonatum Sibiricum</i> Red. Antioxidants <i>Polygonatum sibiricum</i> UF-LC-MS antioxidative anti-hyperuricemic SOD XOD |
| title | Potential Antioxidative and Anti-Hyperuricemic Components Targeting Superoxide Dismutase and Xanthine Oxidase Explored from <i>Polygonatum Sibiricum</i> Red. |
| title_full | Potential Antioxidative and Anti-Hyperuricemic Components Targeting Superoxide Dismutase and Xanthine Oxidase Explored from <i>Polygonatum Sibiricum</i> Red. |
| title_fullStr | Potential Antioxidative and Anti-Hyperuricemic Components Targeting Superoxide Dismutase and Xanthine Oxidase Explored from <i>Polygonatum Sibiricum</i> Red. |
| title_full_unstemmed | Potential Antioxidative and Anti-Hyperuricemic Components Targeting Superoxide Dismutase and Xanthine Oxidase Explored from <i>Polygonatum Sibiricum</i> Red. |
| title_short | Potential Antioxidative and Anti-Hyperuricemic Components Targeting Superoxide Dismutase and Xanthine Oxidase Explored from <i>Polygonatum Sibiricum</i> Red. |
| title_sort | potential antioxidative and anti hyperuricemic components targeting superoxide dismutase and xanthine oxidase explored from i polygonatum sibiricum i red |
| topic | <i>Polygonatum sibiricum</i> UF-LC-MS antioxidative anti-hyperuricemic SOD XOD |
| url | https://www.mdpi.com/2076-3921/11/9/1651 |
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