Dendritic Mesoporous Organosilica Nanoparticles with Photosensitizers for Cell Imaging, siRNA Delivery and Protein Loading
Dendritic mesoporous organosilica nanoparticles (DMON) are a new class of biodegradable nanoparticles suitable for biomolecule delivery. We studied the photochemical internalization (PCI) and photodynamic therapy (PDT) of DMON to investigate new ways for DMON to escape from the endosomes-lysosomes a...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-07-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/14/5335 |
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| author | Haneen Omar Sara Jakimoska Julia Guillot Edreese Alsharaeh Clarence Charnay Frédérique Cunin Aurélie Bessière Jean-Olivier Durand Laurence Raehm Laure Lichon Mélanie Onofre Magali Gary-Bobo |
| author_facet | Haneen Omar Sara Jakimoska Julia Guillot Edreese Alsharaeh Clarence Charnay Frédérique Cunin Aurélie Bessière Jean-Olivier Durand Laurence Raehm Laure Lichon Mélanie Onofre Magali Gary-Bobo |
| author_sort | Haneen Omar |
| collection | DOAJ |
| description | Dendritic mesoporous organosilica nanoparticles (DMON) are a new class of biodegradable nanoparticles suitable for biomolecule delivery. We studied the photochemical internalization (PCI) and photodynamic therapy (PDT) of DMON to investigate new ways for DMON to escape from the endosomes-lysosomes and deliver biomolecules into the cytoplasm of cells. We added photosensitizers in the framework of DMON and found that DMON were loaded with siRNA or FVIII factor protein. We made four formulations with four different photosensitizers. The photosensitizers allowed us to perform imaging of DMON in cancer cells, but the presence of the tetrasulfide bond in the framework of DMON quenched the formation of singlet oxygen. Fortunately, one formulation allowed us to efficiently deliver proapoptotic siRNA in MCF-7 cancer cells leading to 31% of cancer cell death, without irradiation. As for FVIII protein, it was loaded in two formulations with drug-loading capacities (DLC) up to 25%. In conclusion, DMON are versatile nanoparticles capable of loading siRNA and delivering it into cancer cells, and also loading FVIII protein with good DLC. Due to the presence of tetrasulfide, it was not possible to perform PDT or PCI. |
| first_indexed | 2024-03-11T00:47:56Z |
| format | Article |
| id | doaj.art-71fa80dbdbe6429db3e4f8d47ddb4e98 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T00:47:56Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-71fa80dbdbe6429db3e4f8d47ddb4e982023-11-18T20:40:35ZengMDPI AGMolecules1420-30492023-07-012814533510.3390/molecules28145335Dendritic Mesoporous Organosilica Nanoparticles with Photosensitizers for Cell Imaging, siRNA Delivery and Protein LoadingHaneen Omar0Sara Jakimoska1Julia Guillot2Edreese Alsharaeh3Clarence Charnay4Frédérique Cunin5Aurélie Bessière6Jean-Olivier Durand7Laurence Raehm8Laure Lichon9Mélanie Onofre10Magali Gary-Bobo11Chemistry Department, Collage of Science, Alfaisal University, Riyadh 11533, Saudi ArabiaIBMM, University Montpellier, CNRS, ENSCM, 34193 Montpellier, FranceIBMM, University Montpellier, CNRS, ENSCM, 34193 Montpellier, FranceChemistry Department, Collage of Science, Alfaisal University, Riyadh 11533, Saudi ArabiaICGM, University Montpellier, CNRS, ENSCM, 34193 Montpellier, FranceICGM, University Montpellier, CNRS, ENSCM, 34193 Montpellier, FranceICGM, University Montpellier, CNRS, ENSCM, 34193 Montpellier, FranceICGM, University Montpellier, CNRS, ENSCM, 34193 Montpellier, FranceICGM, University Montpellier, CNRS, ENSCM, 34193 Montpellier, FranceIBMM, University Montpellier, CNRS, ENSCM, 34193 Montpellier, FranceIBMM, University Montpellier, CNRS, ENSCM, 34193 Montpellier, FranceIBMM, University Montpellier, CNRS, ENSCM, 34193 Montpellier, FranceDendritic mesoporous organosilica nanoparticles (DMON) are a new class of biodegradable nanoparticles suitable for biomolecule delivery. We studied the photochemical internalization (PCI) and photodynamic therapy (PDT) of DMON to investigate new ways for DMON to escape from the endosomes-lysosomes and deliver biomolecules into the cytoplasm of cells. We added photosensitizers in the framework of DMON and found that DMON were loaded with siRNA or FVIII factor protein. We made four formulations with four different photosensitizers. The photosensitizers allowed us to perform imaging of DMON in cancer cells, but the presence of the tetrasulfide bond in the framework of DMON quenched the formation of singlet oxygen. Fortunately, one formulation allowed us to efficiently deliver proapoptotic siRNA in MCF-7 cancer cells leading to 31% of cancer cell death, without irradiation. As for FVIII protein, it was loaded in two formulations with drug-loading capacities (DLC) up to 25%. In conclusion, DMON are versatile nanoparticles capable of loading siRNA and delivering it into cancer cells, and also loading FVIII protein with good DLC. Due to the presence of tetrasulfide, it was not possible to perform PDT or PCI.https://www.mdpi.com/1420-3049/28/14/5335dendritic mesoporous organosilica nanoparticlessiRNAFVIII factor |
| spellingShingle | Haneen Omar Sara Jakimoska Julia Guillot Edreese Alsharaeh Clarence Charnay Frédérique Cunin Aurélie Bessière Jean-Olivier Durand Laurence Raehm Laure Lichon Mélanie Onofre Magali Gary-Bobo Dendritic Mesoporous Organosilica Nanoparticles with Photosensitizers for Cell Imaging, siRNA Delivery and Protein Loading Molecules dendritic mesoporous organosilica nanoparticles siRNA FVIII factor |
| title | Dendritic Mesoporous Organosilica Nanoparticles with Photosensitizers for Cell Imaging, siRNA Delivery and Protein Loading |
| title_full | Dendritic Mesoporous Organosilica Nanoparticles with Photosensitizers for Cell Imaging, siRNA Delivery and Protein Loading |
| title_fullStr | Dendritic Mesoporous Organosilica Nanoparticles with Photosensitizers for Cell Imaging, siRNA Delivery and Protein Loading |
| title_full_unstemmed | Dendritic Mesoporous Organosilica Nanoparticles with Photosensitizers for Cell Imaging, siRNA Delivery and Protein Loading |
| title_short | Dendritic Mesoporous Organosilica Nanoparticles with Photosensitizers for Cell Imaging, siRNA Delivery and Protein Loading |
| title_sort | dendritic mesoporous organosilica nanoparticles with photosensitizers for cell imaging sirna delivery and protein loading |
| topic | dendritic mesoporous organosilica nanoparticles siRNA FVIII factor |
| url | https://www.mdpi.com/1420-3049/28/14/5335 |
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