Multi-omic analyses of m5C readers reveal their characteristics and immunotherapeutic proficiency
Abstract 5-methylcytosine (m5C) is a post-transcriptional RNA modification identified, m5C readers can specifically identify and bind to m5C. ALYREF and YBX1 as members of m5C readers that have garnered increasing attention in cancer research. However, comprehensive analysis of their molecular funct...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-52110-7 |
| _version_ | 1797350001241751552 |
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| author | Rui Xu Yue Wang Ye Kuang |
| author_facet | Rui Xu Yue Wang Ye Kuang |
| author_sort | Rui Xu |
| collection | DOAJ |
| description | Abstract 5-methylcytosine (m5C) is a post-transcriptional RNA modification identified, m5C readers can specifically identify and bind to m5C. ALYREF and YBX1 as members of m5C readers that have garnered increasing attention in cancer research. However, comprehensive analysis of their molecular functions across pancancer are lacking. Using the TCGA and GTEx databases, we investigated the expression levels and prognostic values of ALYREF and YBX1. Additionally, we assessed the tumor microenvironment, immune checkpoint-related genes, immunomodulators, Tumor Immune Dysfunction and Exclusion (TIDE) score and drug resistance of ALYREF and YBX1. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and Gene Set Enrichment Analysis (GSEA) analyses were performed to investigate the potential functions associated with m5C readers and coexpressed genes. Aberrant expression of ALYREF and YBX1 was observed and positively associated with prognosis in KIRP, LGG and LIHC. Furthermore, the expression levels of ALYREF and YBX1 were significantly correlated with immune infiltration of the tumor microenvironment and immune-related modulators. Last, our analysis revealed significant correlations between ALYREF, YBX1 and eIFs. Our study provides a substantial understanding of m5C readers and the intricate relationship between ALYREF, YBX1, eIFs, and mRNA dynamics. Through multidimensional analysis of immune infiltration and drug sensitivity/resistance in ALYREF and YBX1, we propose a possibility for combined modality therapy utilizing m5C readers. |
| first_indexed | 2024-03-08T12:38:29Z |
| format | Article |
| id | doaj.art-71fc01d3b894453dbfce2e9a57349663 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-08T12:38:29Z |
| publishDate | 2024-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-71fc01d3b894453dbfce2e9a573496632024-01-21T12:17:24ZengNature PortfolioScientific Reports2045-23222024-01-0114111510.1038/s41598-024-52110-7Multi-omic analyses of m5C readers reveal their characteristics and immunotherapeutic proficiencyRui Xu0Yue Wang1Ye Kuang2Department of Development Planning, International Medical Opening-up Pilot Zone (China)Department of Endocrinology, The First Affiliated Hospital of Dalian Medical UniversityDepartment of Medical Laboratory, Yan’An Hospital of Kunming CityAbstract 5-methylcytosine (m5C) is a post-transcriptional RNA modification identified, m5C readers can specifically identify and bind to m5C. ALYREF and YBX1 as members of m5C readers that have garnered increasing attention in cancer research. However, comprehensive analysis of their molecular functions across pancancer are lacking. Using the TCGA and GTEx databases, we investigated the expression levels and prognostic values of ALYREF and YBX1. Additionally, we assessed the tumor microenvironment, immune checkpoint-related genes, immunomodulators, Tumor Immune Dysfunction and Exclusion (TIDE) score and drug resistance of ALYREF and YBX1. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and Gene Set Enrichment Analysis (GSEA) analyses were performed to investigate the potential functions associated with m5C readers and coexpressed genes. Aberrant expression of ALYREF and YBX1 was observed and positively associated with prognosis in KIRP, LGG and LIHC. Furthermore, the expression levels of ALYREF and YBX1 were significantly correlated with immune infiltration of the tumor microenvironment and immune-related modulators. Last, our analysis revealed significant correlations between ALYREF, YBX1 and eIFs. Our study provides a substantial understanding of m5C readers and the intricate relationship between ALYREF, YBX1, eIFs, and mRNA dynamics. Through multidimensional analysis of immune infiltration and drug sensitivity/resistance in ALYREF and YBX1, we propose a possibility for combined modality therapy utilizing m5C readers.https://doi.org/10.1038/s41598-024-52110-7 |
| spellingShingle | Rui Xu Yue Wang Ye Kuang Multi-omic analyses of m5C readers reveal their characteristics and immunotherapeutic proficiency Scientific Reports |
| title | Multi-omic analyses of m5C readers reveal their characteristics and immunotherapeutic proficiency |
| title_full | Multi-omic analyses of m5C readers reveal their characteristics and immunotherapeutic proficiency |
| title_fullStr | Multi-omic analyses of m5C readers reveal their characteristics and immunotherapeutic proficiency |
| title_full_unstemmed | Multi-omic analyses of m5C readers reveal their characteristics and immunotherapeutic proficiency |
| title_short | Multi-omic analyses of m5C readers reveal their characteristics and immunotherapeutic proficiency |
| title_sort | multi omic analyses of m5c readers reveal their characteristics and immunotherapeutic proficiency |
| url | https://doi.org/10.1038/s41598-024-52110-7 |
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