Optimised Method for Short-Chain Fatty Acid Profiling of Bovine Milk and Serum
Short-chain fatty acids (SCFA, C2-C5) in milk and serum are derived from rumen bacterial fermentation and, thus, have the potential to be used as biomarkers for the health status of dairy cows. Currently, there is no comprehensive and validated method that can be used to analyse all SCFAs in both bo...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-01-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/2/436 |
| _version_ | 1797491555950395392 |
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| author | Cheng Li Zhiqian Liu Carolyn Bath Leah Marett Jennie Pryce Simone Rochfort |
| author_facet | Cheng Li Zhiqian Liu Carolyn Bath Leah Marett Jennie Pryce Simone Rochfort |
| author_sort | Cheng Li |
| collection | DOAJ |
| description | Short-chain fatty acids (SCFA, C2-C5) in milk and serum are derived from rumen bacterial fermentation and, thus, have the potential to be used as biomarkers for the health status of dairy cows. Currently, there is no comprehensive and validated method that can be used to analyse all SCFAs in both bovine serum and milk. This paper reports an optimised protocol, combining 3-nitrophenylhydrazine (3-NPH) derivatisation and liquid chromatography-mass spectrometry (LC-MS) analysis for quantification of SCFA and β-hydroxybutyric acid (BHBA) in both bovine milk and bovine serum. This method is sensitive (limit of detection (LOD) ≤ 0.1 µmol/L of bovine milk and serum), accurate (recovery 84–115% for most analytes) and reproducible (relative standard deviation (RSD) for repeated analyses below 7% for most measurements) with a short sample preparation step. The application of this method to samples collected from a small cohort of animals allowed us to reveal a large variation in SCFA concentration between serum and milk and across different animals as well as the strong correlation of some SCFAs between milk and serum samples. |
| first_indexed | 2024-03-10T00:50:58Z |
| format | Article |
| id | doaj.art-72037682049242358d2f0761a0d11211 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T00:50:58Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-72037682049242358d2f0761a0d112112023-11-23T14:51:55ZengMDPI AGMolecules1420-30492022-01-0127243610.3390/molecules27020436Optimised Method for Short-Chain Fatty Acid Profiling of Bovine Milk and SerumCheng Li0Zhiqian Liu1Carolyn Bath2Leah Marett3Jennie Pryce4Simone Rochfort5Agriculture Victoria Research, AgriBio, 5 Ring Road, Bundoora, VIC 3083, AustraliaAgriculture Victoria Research, AgriBio, 5 Ring Road, Bundoora, VIC 3083, AustraliaAgriculture Victoria Research, AgriBio, 5 Ring Road, Bundoora, VIC 3083, AustraliaAgriculture Victoria Research, Ellinbank Centre, Ellinbank, VIC 3821, AustraliaAgriculture Victoria Research, AgriBio, 5 Ring Road, Bundoora, VIC 3083, AustraliaAgriculture Victoria Research, AgriBio, 5 Ring Road, Bundoora, VIC 3083, AustraliaShort-chain fatty acids (SCFA, C2-C5) in milk and serum are derived from rumen bacterial fermentation and, thus, have the potential to be used as biomarkers for the health status of dairy cows. Currently, there is no comprehensive and validated method that can be used to analyse all SCFAs in both bovine serum and milk. This paper reports an optimised protocol, combining 3-nitrophenylhydrazine (3-NPH) derivatisation and liquid chromatography-mass spectrometry (LC-MS) analysis for quantification of SCFA and β-hydroxybutyric acid (BHBA) in both bovine milk and bovine serum. This method is sensitive (limit of detection (LOD) ≤ 0.1 µmol/L of bovine milk and serum), accurate (recovery 84–115% for most analytes) and reproducible (relative standard deviation (RSD) for repeated analyses below 7% for most measurements) with a short sample preparation step. The application of this method to samples collected from a small cohort of animals allowed us to reveal a large variation in SCFA concentration between serum and milk and across different animals as well as the strong correlation of some SCFAs between milk and serum samples.https://www.mdpi.com/1420-3049/27/2/436bovine milkserumshort-chain fatty acidsliquid chromatography-mass spectrometry |
| spellingShingle | Cheng Li Zhiqian Liu Carolyn Bath Leah Marett Jennie Pryce Simone Rochfort Optimised Method for Short-Chain Fatty Acid Profiling of Bovine Milk and Serum Molecules bovine milk serum short-chain fatty acids liquid chromatography-mass spectrometry |
| title | Optimised Method for Short-Chain Fatty Acid Profiling of Bovine Milk and Serum |
| title_full | Optimised Method for Short-Chain Fatty Acid Profiling of Bovine Milk and Serum |
| title_fullStr | Optimised Method for Short-Chain Fatty Acid Profiling of Bovine Milk and Serum |
| title_full_unstemmed | Optimised Method for Short-Chain Fatty Acid Profiling of Bovine Milk and Serum |
| title_short | Optimised Method for Short-Chain Fatty Acid Profiling of Bovine Milk and Serum |
| title_sort | optimised method for short chain fatty acid profiling of bovine milk and serum |
| topic | bovine milk serum short-chain fatty acids liquid chromatography-mass spectrometry |
| url | https://www.mdpi.com/1420-3049/27/2/436 |
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