Optimum Conditions Of 5-Nucleotidase Activity In Sera Of KalaAzaric Patients.
Background: This research focuses on the characterization studies of 5v-Nucleotidase (5'-NT) of kalaazar patients' sera. 5V NT is one of the enzymes used as indicator of liver function test . In this study the optimum conditions for the activity of 5s - NT enzyme has been demonstrated for...
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| Format: | Article |
| Language: | English |
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College of Medicine University of Baghdad
2006-10-01
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| Series: | مجلة كلية الطب |
| Subjects: | |
| Online Access: | http://iqjmc.uobaghdad.edu.iq/index.php/19JFacMedBaghdad36/article/view/1513 |
| _version_ | 1797369942750789632 |
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| author | Eaman A.S. AL-Rubaee |
| author_facet | Eaman A.S. AL-Rubaee |
| author_sort | Eaman A.S. AL-Rubaee |
| collection | DOAJ |
| description | Background: This research focuses on the characterization studies of 5v-Nucleotidase (5'-NT) of kalaazar patients' sera. 5V NT is one of the enzymes used as indicator of liver function test . In this study the optimum conditions for the activity of 5s - NT enzyme has been demonstrated for kalaazaric patients.
Materials and methods: Thirty five blood samples were obtained from Baghdad Hospitals from untreated infants and children with kala-azar. 5'-NT activity was measured by following the
method of Wood and Williams.
Results: Optimum conditions of serum 5' - NT activity of kala- azaric patients obtained were 50 u L , 30 minutes ,0.8 mM,7.7 and 50'C) as (Volume of serum , incubation time , substrate
concentration, pH and temperature) respectively |
| first_indexed | 2024-03-08T17:54:16Z |
| format | Article |
| id | doaj.art-7203cca6857440869c5d3a28333f62db |
| institution | Directory Open Access Journal |
| issn | 0041-9419 2410-8057 |
| language | English |
| last_indexed | 2024-03-08T17:54:16Z |
| publishDate | 2006-10-01 |
| publisher | College of Medicine University of Baghdad |
| record_format | Article |
| series | مجلة كلية الطب |
| spelling | doaj.art-7203cca6857440869c5d3a28333f62db2024-01-02T05:56:37ZengCollege of Medicine University of Baghdadمجلة كلية الطب0041-94192410-80572006-10-01483Optimum Conditions Of 5-Nucleotidase Activity In Sera Of KalaAzaric Patients.Eaman A.S. AL-Rubaee0Basic Science Dept., Collage of Dentistry, University of Baghdad.Background: This research focuses on the characterization studies of 5v-Nucleotidase (5'-NT) of kalaazar patients' sera. 5V NT is one of the enzymes used as indicator of liver function test . In this study the optimum conditions for the activity of 5s - NT enzyme has been demonstrated for kalaazaric patients. Materials and methods: Thirty five blood samples were obtained from Baghdad Hospitals from untreated infants and children with kala-azar. 5'-NT activity was measured by following the method of Wood and Williams. Results: Optimum conditions of serum 5' - NT activity of kala- azaric patients obtained were 50 u L , 30 minutes ,0.8 mM,7.7 and 50'C) as (Volume of serum , incubation time , substrate concentration, pH and temperature) respectivelyhttp://iqjmc.uobaghdad.edu.iq/index.php/19JFacMedBaghdad36/article/view/15135 -Nuceotidase, serum and kala-azar. |
| spellingShingle | Eaman A.S. AL-Rubaee Optimum Conditions Of 5-Nucleotidase Activity In Sera Of KalaAzaric Patients. مجلة كلية الطب 5 -Nuceotidase, serum and kala-azar. |
| title | Optimum Conditions Of 5-Nucleotidase Activity In Sera Of KalaAzaric Patients. |
| title_full | Optimum Conditions Of 5-Nucleotidase Activity In Sera Of KalaAzaric Patients. |
| title_fullStr | Optimum Conditions Of 5-Nucleotidase Activity In Sera Of KalaAzaric Patients. |
| title_full_unstemmed | Optimum Conditions Of 5-Nucleotidase Activity In Sera Of KalaAzaric Patients. |
| title_short | Optimum Conditions Of 5-Nucleotidase Activity In Sera Of KalaAzaric Patients. |
| title_sort | optimum conditions of 5 nucleotidase activity in sera of kalaazaric patients |
| topic | 5 -Nuceotidase, serum and kala-azar. |
| url | http://iqjmc.uobaghdad.edu.iq/index.php/19JFacMedBaghdad36/article/view/1513 |
| work_keys_str_mv | AT eamanasalrubaee optimumconditionsof5nucleotidaseactivityinseraofkalaazaricpatients |