Detection of genes involved in biofilm formation in Staphylococcus aureus isolates
is one of the Gram-positive pathogens causing a wide range of nosocomial infections. The present study investigates genotypic and phenotypic aspects involved in biofilm formation in methicillin-resistant strains isolated from nosocomial infections in Isfahan. A total of 110 strains were collected...
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| Format: | Article |
| Language: | deu |
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German Medical Science GMS Publishing House
2016-03-01
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| Series: | GMS Hygiene and Infection Control |
| Subjects: | |
| Online Access: | http://www.egms.de/static/en/journals/dgkh/2016-11/dgkh000267.shtml |
| _version_ | 1819227814002425856 |
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| author | Nourbakhsh, Fahimeh Namvar, Amirmorteza Ebrahimzadeh |
| author_facet | Nourbakhsh, Fahimeh Namvar, Amirmorteza Ebrahimzadeh |
| author_sort | Nourbakhsh, Fahimeh |
| collection | DOAJ |
| description | is one of the Gram-positive pathogens causing a wide range of nosocomial infections. The present study investigates genotypic and phenotypic aspects involved in biofilm formation in methicillin-resistant strains isolated from nosocomial infections in Isfahan. A total of 110 strains were collected from three major hospitals in Isfahan, the center of Iran. The antibiotic resistance pattern, phenotypes, and biofilm formation genes were studied using Congo red agar (CRA) and multiplex PCR (M-PCR). We found that 103 out of 110 samples (93.6%) were MRSA. The highest frequency of resistance was found to penicillin (89%), ciprofloxacin (87.4%), and erythromycin (86.1%). Phenotypic results showed that 53.5% were high biofilm producers, while 33.3% and 13.2% were intermediate and low biofilm producers, respectively. (69.3%) had the highest frequency in comparison to other intercellular adhesion () genes, (54.8%) was second most common.The results show that the adherence or attachment ability and biofilm production are important for enhancing virulence factors among isolates of strains. |
| first_indexed | 2024-12-23T10:47:21Z |
| format | Article |
| id | doaj.art-720ae0742d4d4b429dc8464a7431fb46 |
| institution | Directory Open Access Journal |
| issn | 2196-5226 |
| language | deu |
| last_indexed | 2024-12-23T10:47:21Z |
| publishDate | 2016-03-01 |
| publisher | German Medical Science GMS Publishing House |
| record_format | Article |
| series | GMS Hygiene and Infection Control |
| spelling | doaj.art-720ae0742d4d4b429dc8464a7431fb462022-12-21T17:49:59ZdeuGerman Medical Science GMS Publishing HouseGMS Hygiene and Infection Control2196-52262016-03-0111Doc0710.3205/dgkh000267Detection of genes involved in biofilm formation in Staphylococcus aureus isolatesNourbakhsh, Fahimeh0Namvar, Amirmorteza Ebrahimzadeh1Department of Microbiology, Islamic Azad University, Shahrekord branch, Shahrekord, IranDepartment of Microbiology, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iranis one of the Gram-positive pathogens causing a wide range of nosocomial infections. The present study investigates genotypic and phenotypic aspects involved in biofilm formation in methicillin-resistant strains isolated from nosocomial infections in Isfahan. A total of 110 strains were collected from three major hospitals in Isfahan, the center of Iran. The antibiotic resistance pattern, phenotypes, and biofilm formation genes were studied using Congo red agar (CRA) and multiplex PCR (M-PCR). We found that 103 out of 110 samples (93.6%) were MRSA. The highest frequency of resistance was found to penicillin (89%), ciprofloxacin (87.4%), and erythromycin (86.1%). Phenotypic results showed that 53.5% were high biofilm producers, while 33.3% and 13.2% were intermediate and low biofilm producers, respectively. (69.3%) had the highest frequency in comparison to other intercellular adhesion () genes, (54.8%) was second most common.The results show that the adherence or attachment ability and biofilm production are important for enhancing virulence factors among isolates of strains.http://www.egms.de/static/en/journals/dgkh/2016-11/dgkh000267.shtmlStaphylococcus aureusMRSAbiofilmica geneantibiotic resistance |
| spellingShingle | Nourbakhsh, Fahimeh Namvar, Amirmorteza Ebrahimzadeh Detection of genes involved in biofilm formation in Staphylococcus aureus isolates GMS Hygiene and Infection Control Staphylococcus aureus MRSA biofilm ica gene antibiotic resistance |
| title | Detection of genes involved in biofilm formation in Staphylococcus aureus isolates |
| title_full | Detection of genes involved in biofilm formation in Staphylococcus aureus isolates |
| title_fullStr | Detection of genes involved in biofilm formation in Staphylococcus aureus isolates |
| title_full_unstemmed | Detection of genes involved in biofilm formation in Staphylococcus aureus isolates |
| title_short | Detection of genes involved in biofilm formation in Staphylococcus aureus isolates |
| title_sort | detection of genes involved in biofilm formation in staphylococcus aureus isolates |
| topic | Staphylococcus aureus MRSA biofilm ica gene antibiotic resistance |
| url | http://www.egms.de/static/en/journals/dgkh/2016-11/dgkh000267.shtml |
| work_keys_str_mv | AT nourbakhshfahimeh detectionofgenesinvolvedinbiofilmformationinstaphylococcusaureusisolates AT namvaramirmortezaebrahimzadeh detectionofgenesinvolvedinbiofilmformationinstaphylococcusaureusisolates |