Zika virus and temperature modulate Elizabethkingia anophelis in Aedes albopictus
Abstract Background Vector-borne pathogens must survive and replicate in the hostile environment of an insect’s midgut before successful dissemination. Midgut microbiota interfere with pathogen infection by activating the basal immunity of the mosquito and by synthesizing pathogen-inhibitory metabol...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-11-01
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| Series: | Parasites & Vectors |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13071-021-05069-7 |
| _version_ | 1818837865262481408 |
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| author | Maria G. Onyango Rachel Lange Sean Bialosuknia Anne Payne Nicholas Mathias Lili Kuo Aurelien Vigneron Dilip Nag Laura D. Kramer Alexander T. Ciota |
| author_facet | Maria G. Onyango Rachel Lange Sean Bialosuknia Anne Payne Nicholas Mathias Lili Kuo Aurelien Vigneron Dilip Nag Laura D. Kramer Alexander T. Ciota |
| author_sort | Maria G. Onyango |
| collection | DOAJ |
| description | Abstract Background Vector-borne pathogens must survive and replicate in the hostile environment of an insect’s midgut before successful dissemination. Midgut microbiota interfere with pathogen infection by activating the basal immunity of the mosquito and by synthesizing pathogen-inhibitory metabolites. Methods The goal of this study was to assess the influence of Zika virus (ZIKV) infection and increased temperature on Aedes albopictus midgut microbiota. Aedes albopictus were reared at diurnal temperatures of day 28 °C/night 24 °C (L) or day 30 °C/night 26 °C (M). The mosquitoes were given infectious blood meals with 2.0 × 108 PFU/ml ZIKV, and 16S rRNA sequencing was performed on midguts at 7 days post-infectious blood meal exposure. Results Our findings demonstrate that Elizabethkingia anophelis albopictus was associated with Ae. albopictus midguts exposed to ZIKV infectious blood meal. We observed a negative correlation between ZIKV and E. anophelis albopictus in the midguts of Ae. albopictus. Supplemental feeding of Ae. albopictus with E. anophelis aegypti and ZIKV resulted in reduced ZIKV infection rates. Reduced viral loads were detected in Vero cells that were sequentially infected with E. anophelis aegypti and ZIKV, dengue virus (DENV), or chikungunya virus (CHIKV). Conclusions Our findings demonstrate the influence of ZIKV infection and temperature on the Ae. albopictus microbiome along with a negative correlation between ZIKV and E. anophelis albopictus. Our results have important implications for controlling vector-borne pathogens. Graphical Abstract |
| first_indexed | 2024-12-19T03:29:17Z |
| format | Article |
| id | doaj.art-720f45cc46b2476695f187be4d974cde |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-12-19T03:29:17Z |
| publishDate | 2021-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-720f45cc46b2476695f187be4d974cde2022-12-21T20:37:33ZengBMCParasites & Vectors1756-33052021-11-0114111510.1186/s13071-021-05069-7Zika virus and temperature modulate Elizabethkingia anophelis in Aedes albopictusMaria G. Onyango0Rachel Lange1Sean Bialosuknia2Anne Payne3Nicholas Mathias4Lili Kuo5Aurelien Vigneron6Dilip Nag7Laura D. Kramer8Alexander T. Ciota9Department of Biological Sciences, College of Arts and Sciences, Texas Tech UniversityArbovirus Laboratory, Wadsworth Center, New York State Department of HealthArbovirus Laboratory, Wadsworth Center, New York State Department of HealthArbovirus Laboratory, Wadsworth Center, New York State Department of HealthArbovirus Laboratory, Wadsworth Center, New York State Department of HealthArbovirus Laboratory, Wadsworth Center, New York State Department of HealthInstitute of Organismic and Molecular Evolution, Johannes Gutenberg University MainzArbovirus Laboratory, Wadsworth Center, New York State Department of HealthArbovirus Laboratory, Wadsworth Center, New York State Department of HealthArbovirus Laboratory, Wadsworth Center, New York State Department of HealthAbstract Background Vector-borne pathogens must survive and replicate in the hostile environment of an insect’s midgut before successful dissemination. Midgut microbiota interfere with pathogen infection by activating the basal immunity of the mosquito and by synthesizing pathogen-inhibitory metabolites. Methods The goal of this study was to assess the influence of Zika virus (ZIKV) infection and increased temperature on Aedes albopictus midgut microbiota. Aedes albopictus were reared at diurnal temperatures of day 28 °C/night 24 °C (L) or day 30 °C/night 26 °C (M). The mosquitoes were given infectious blood meals with 2.0 × 108 PFU/ml ZIKV, and 16S rRNA sequencing was performed on midguts at 7 days post-infectious blood meal exposure. Results Our findings demonstrate that Elizabethkingia anophelis albopictus was associated with Ae. albopictus midguts exposed to ZIKV infectious blood meal. We observed a negative correlation between ZIKV and E. anophelis albopictus in the midguts of Ae. albopictus. Supplemental feeding of Ae. albopictus with E. anophelis aegypti and ZIKV resulted in reduced ZIKV infection rates. Reduced viral loads were detected in Vero cells that were sequentially infected with E. anophelis aegypti and ZIKV, dengue virus (DENV), or chikungunya virus (CHIKV). Conclusions Our findings demonstrate the influence of ZIKV infection and temperature on the Ae. albopictus microbiome along with a negative correlation between ZIKV and E. anophelis albopictus. Our results have important implications for controlling vector-borne pathogens. Graphical Abstracthttps://doi.org/10.1186/s13071-021-05069-7Zika virusAedes albopictusMicrobiomeElizabethkingia |
| spellingShingle | Maria G. Onyango Rachel Lange Sean Bialosuknia Anne Payne Nicholas Mathias Lili Kuo Aurelien Vigneron Dilip Nag Laura D. Kramer Alexander T. Ciota Zika virus and temperature modulate Elizabethkingia anophelis in Aedes albopictus Parasites & Vectors Zika virus Aedes albopictus Microbiome Elizabethkingia |
| title | Zika virus and temperature modulate Elizabethkingia anophelis in Aedes albopictus |
| title_full | Zika virus and temperature modulate Elizabethkingia anophelis in Aedes albopictus |
| title_fullStr | Zika virus and temperature modulate Elizabethkingia anophelis in Aedes albopictus |
| title_full_unstemmed | Zika virus and temperature modulate Elizabethkingia anophelis in Aedes albopictus |
| title_short | Zika virus and temperature modulate Elizabethkingia anophelis in Aedes albopictus |
| title_sort | zika virus and temperature modulate elizabethkingia anophelis in aedes albopictus |
| topic | Zika virus Aedes albopictus Microbiome Elizabethkingia |
| url | https://doi.org/10.1186/s13071-021-05069-7 |
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