β-Carotene from the Alga <i>Dunaliella bardawil</i> Decreases Gene Expression of Adipose Tissue Macrophage Recruitment Markers and Plasma Lipid Concentrations in Mice Fed a High-Fat Diet
Vitamin A and provitamin A carotenoids are involved in the regulation of adipose tissue metabolism and inflammation. We examined the effect of dietary supplementation using all-trans and 9-cis β-carotene-rich <i>Dunaliella bardawil</i> alga as the sole source of vitamin A on obesity-asso...
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MDPI AG
2022-06-01
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| Online Access: | https://www.mdpi.com/1660-3397/20/7/433 |
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| author | Nir Melnikov Yehuda Kamari Michal Kandel-Kfir Iris Barshack Ami Ben-Amotz Dror Harats Aviv Shaish Ayelet Harari |
| author_facet | Nir Melnikov Yehuda Kamari Michal Kandel-Kfir Iris Barshack Ami Ben-Amotz Dror Harats Aviv Shaish Ayelet Harari |
| author_sort | Nir Melnikov |
| collection | DOAJ |
| description | Vitamin A and provitamin A carotenoids are involved in the regulation of adipose tissue metabolism and inflammation. We examined the effect of dietary supplementation using all-trans and 9-cis β-carotene-rich <i>Dunaliella bardawil</i> alga as the sole source of vitamin A on obesity-associated comorbidities and adipose tissue dysfunction in a diet-induced obesity mouse model. Three-week-old male mice (C57BL/6) were randomly allocated into two groups and fed a high-fat, vitamin A-deficient diet supplemented with either vitamin A (HFD) or β-carotene (BC) (HFD-BC). Vitamin A levels in the liver, WATs, and BAT of the HFD-BC group were 1.5–2.4-fold higher than of the HFD group. BC concentrations were 5–6-fold greater in BAT compared to WAT in the HFD-BC group. The eWAT mRNA levels of the <i>Mcp-1</i> and <i>Cd68</i> were 1.6- and 2.1-fold lower, respectively, and the plasma cholesterol and triglyceride concentrations were 30% and 28% lower in the HFD-BC group compared with the HFD group. Dietary BC can be the exclusive vitamin A source in mice fed a high-fat diet, as shown by the vitamin A concentration in the plasma and tissues. Feeding BC rather than vitamin A reduces adipose tissue macrophage recruitment markers and plasma lipid concentrations. |
| first_indexed | 2024-03-09T10:15:28Z |
| format | Article |
| id | doaj.art-72108c61089548e5bb9b43f1de601479 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-09T10:15:28Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-72108c61089548e5bb9b43f1de6014792023-12-01T22:23:43ZengMDPI AGMarine Drugs1660-33972022-06-0120743310.3390/md20070433β-Carotene from the Alga <i>Dunaliella bardawil</i> Decreases Gene Expression of Adipose Tissue Macrophage Recruitment Markers and Plasma Lipid Concentrations in Mice Fed a High-Fat DietNir Melnikov0Yehuda Kamari1Michal Kandel-Kfir2Iris Barshack3Ami Ben-Amotz4Dror Harats5Aviv Shaish6Ayelet Harari7The Bert W. Strassburger Metabolic Center, Sheba Medical Center, Ramat Gan 5262000, IsraelThe Bert W. Strassburger Metabolic Center, Sheba Medical Center, Ramat Gan 5262000, IsraelThe Bert W. Strassburger Metabolic Center, Sheba Medical Center, Ramat Gan 5262000, IsraelThe Bert W. Strassburger Metabolic Center, Sheba Medical Center, Ramat Gan 5262000, IsraelN.B.T., Nature Beta Technologies Ltd., Eilat 8851100, IsraelThe Bert W. Strassburger Metabolic Center, Sheba Medical Center, Ramat Gan 5262000, IsraelThe Bert W. Strassburger Metabolic Center, Sheba Medical Center, Ramat Gan 5262000, IsraelThe Bert W. Strassburger Metabolic Center, Sheba Medical Center, Ramat Gan 5262000, IsraelVitamin A and provitamin A carotenoids are involved in the regulation of adipose tissue metabolism and inflammation. We examined the effect of dietary supplementation using all-trans and 9-cis β-carotene-rich <i>Dunaliella bardawil</i> alga as the sole source of vitamin A on obesity-associated comorbidities and adipose tissue dysfunction in a diet-induced obesity mouse model. Three-week-old male mice (C57BL/6) were randomly allocated into two groups and fed a high-fat, vitamin A-deficient diet supplemented with either vitamin A (HFD) or β-carotene (BC) (HFD-BC). Vitamin A levels in the liver, WATs, and BAT of the HFD-BC group were 1.5–2.4-fold higher than of the HFD group. BC concentrations were 5–6-fold greater in BAT compared to WAT in the HFD-BC group. The eWAT mRNA levels of the <i>Mcp-1</i> and <i>Cd68</i> were 1.6- and 2.1-fold lower, respectively, and the plasma cholesterol and triglyceride concentrations were 30% and 28% lower in the HFD-BC group compared with the HFD group. Dietary BC can be the exclusive vitamin A source in mice fed a high-fat diet, as shown by the vitamin A concentration in the plasma and tissues. Feeding BC rather than vitamin A reduces adipose tissue macrophage recruitment markers and plasma lipid concentrations.https://www.mdpi.com/1660-3397/20/7/433vitamin Aβ-caroteneobesityadipose tissuemice |
| spellingShingle | Nir Melnikov Yehuda Kamari Michal Kandel-Kfir Iris Barshack Ami Ben-Amotz Dror Harats Aviv Shaish Ayelet Harari β-Carotene from the Alga <i>Dunaliella bardawil</i> Decreases Gene Expression of Adipose Tissue Macrophage Recruitment Markers and Plasma Lipid Concentrations in Mice Fed a High-Fat Diet Marine Drugs vitamin A β-carotene obesity adipose tissue mice |
| title | β-Carotene from the Alga <i>Dunaliella bardawil</i> Decreases Gene Expression of Adipose Tissue Macrophage Recruitment Markers and Plasma Lipid Concentrations in Mice Fed a High-Fat Diet |
| title_full | β-Carotene from the Alga <i>Dunaliella bardawil</i> Decreases Gene Expression of Adipose Tissue Macrophage Recruitment Markers and Plasma Lipid Concentrations in Mice Fed a High-Fat Diet |
| title_fullStr | β-Carotene from the Alga <i>Dunaliella bardawil</i> Decreases Gene Expression of Adipose Tissue Macrophage Recruitment Markers and Plasma Lipid Concentrations in Mice Fed a High-Fat Diet |
| title_full_unstemmed | β-Carotene from the Alga <i>Dunaliella bardawil</i> Decreases Gene Expression of Adipose Tissue Macrophage Recruitment Markers and Plasma Lipid Concentrations in Mice Fed a High-Fat Diet |
| title_short | β-Carotene from the Alga <i>Dunaliella bardawil</i> Decreases Gene Expression of Adipose Tissue Macrophage Recruitment Markers and Plasma Lipid Concentrations in Mice Fed a High-Fat Diet |
| title_sort | β carotene from the alga i dunaliella bardawil i decreases gene expression of adipose tissue macrophage recruitment markers and plasma lipid concentrations in mice fed a high fat diet |
| topic | vitamin A β-carotene obesity adipose tissue mice |
| url | https://www.mdpi.com/1660-3397/20/7/433 |
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