Enhancement of fungal DNA templates and PCR amplification yield by three types of nanoparticles
Nanodiagonastic methods in plant pathology are used for enhancing detection and identification of different plant pathogens and toxigenic fungi. Improvement of the specificity and efficiency of the polymerase chain reaction (PCR) by using some nanoparticles is emerging as a new area of research....
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Polish Academy of Sciences
2018-03-01
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| Series: | Journal of Plant Protection Research |
| Subjects: | |
| Online Access: | https://doi.org/10.24425/119119 |
| Summary: | Nanodiagonastic methods in plant pathology are used for enhancing detection and identification
of different plant pathogens and toxigenic fungi. Improvement of the specificity and
efficiency of the polymerase chain reaction (PCR) by using some nanoparticles is emerging
as a new area of research. In the current research, silver, zinc, and gold nanoparticles
were used to increase the yield of DNA for two plant pathogenic fungi including soil-borne
fungus Rhizoctonia solani and toxigenic fungus Alternaria alternata. Gold nanoparticles
combined with zinc and silver nanoparticles enhanced both DNA yield and PCR products
compared to DNA extraction methods with ALB buffer, sodium dodecyl sulfate, ALBfree
from protinase K, ZnNPs and AgNPs. Also, by using ZnNPs and AgNPs the DNA yield
was enhanced and the sensitivity of random amplified polymorphic DNA (RAPD) PCR
products was increased. Application of nanomaterials in the PCR reaction could increase
or decrease the PCR product according to the type of applied nanometal and the type of
DNA template. Additions of AuNPs to PCR mix increased both sensitivity and specificity
for PCR products of the tested fungi. Thus, the use of these highly stable, commercially
available and inexpensive inorganic nano reagents open new opportunities for improving
the specificity and sensitivity of PCR amplicon, which is the most important standard
method in molecular plant pathology and mycotoxicology. |
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| ISSN: | 1899-007X 1899-007X |