Simultaneous quantification of the major flavonoids from wild spinach by UHPLC-HRMS and their neuroprotective effects in a model of H2O2-induced oxidative stress on SH-SY5Y cells
A modified UHPLC-HRMS method for simultaneous quantification of eight flavonoids from the aerial parts of the wild spinach (Chenopodium bonus-henricus L.) was re-validated for specificity, the limit of detection and quantitation limit, linearity, accuracy, and precision. The glycosides of spinacetin...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Pensoft Publishers
2021-09-01
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| Series: | Pharmacia |
| Online Access: | https://pharmacia.pensoft.net/article/71030/download/pdf/ |
| _version_ | 1811329459065716736 |
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| author | Zlatina Kokanova-Nedialkova Denitsa Aluani Virginia Tzankova Paraskev Nedialkov |
| author_facet | Zlatina Kokanova-Nedialkova Denitsa Aluani Virginia Tzankova Paraskev Nedialkov |
| author_sort | Zlatina Kokanova-Nedialkova |
| collection | DOAJ |
| description | A modified UHPLC-HRMS method for simultaneous quantification of eight flavonoids from the aerial parts of the wild spinach (Chenopodium bonus-henricus L.) was re-validated for specificity, the limit of detection and quantitation limit, linearity, accuracy, and precision. The glycosides of spinacetin (Chbhnf-04, Chbhnf-06, and Chbhnf-08) and patuletin (Chbhnf-01) were the predominant compounds. The total amount of assayed flavonoids from the aerial parts of a title plant was estimated to be 1.82% and 1.4% in two different populations from Vitosha Mountain (Bulgaria). The neuroprotective properties of compounds Chbhnf-02, Chbhnf-04, Chbhnf-06, Chbhnf-07, Chbhnf-08 were further assessed using a model of H2O2-induced oxidative stress on human neuroblastoma SH-SY5Y cells. All tested flavonoids demonstrated statistically significant neuroprotective activity close to that of silibinin. Patuletin (Chbhnf-07) and spinacetin (Chbhnf-08) triglycosides showed the most protective effects at the lowest concentration of 50 µM. |
| first_indexed | 2024-04-13T15:45:23Z |
| format | Article |
| id | doaj.art-7211dc92e0494aa6b7ce0664740373e1 |
| institution | Directory Open Access Journal |
| issn | 2603-557X |
| language | English |
| last_indexed | 2024-04-13T15:45:23Z |
| publishDate | 2021-09-01 |
| publisher | Pensoft Publishers |
| record_format | Article |
| series | Pharmacia |
| spelling | doaj.art-7211dc92e0494aa6b7ce0664740373e12022-12-22T02:41:01ZengPensoft PublishersPharmacia2603-557X2021-09-0168365766410.3897/pharmacia.68.e7103071030Simultaneous quantification of the major flavonoids from wild spinach by UHPLC-HRMS and their neuroprotective effects in a model of H2O2-induced oxidative stress on SH-SY5Y cellsZlatina Kokanova-Nedialkova0Denitsa Aluani1Virginia Tzankova2Paraskev Nedialkov3Medical University of SofiaMedical University of SofiaMedical University of SofiaMedical University of SofiaA modified UHPLC-HRMS method for simultaneous quantification of eight flavonoids from the aerial parts of the wild spinach (Chenopodium bonus-henricus L.) was re-validated for specificity, the limit of detection and quantitation limit, linearity, accuracy, and precision. The glycosides of spinacetin (Chbhnf-04, Chbhnf-06, and Chbhnf-08) and patuletin (Chbhnf-01) were the predominant compounds. The total amount of assayed flavonoids from the aerial parts of a title plant was estimated to be 1.82% and 1.4% in two different populations from Vitosha Mountain (Bulgaria). The neuroprotective properties of compounds Chbhnf-02, Chbhnf-04, Chbhnf-06, Chbhnf-07, Chbhnf-08 were further assessed using a model of H2O2-induced oxidative stress on human neuroblastoma SH-SY5Y cells. All tested flavonoids demonstrated statistically significant neuroprotective activity close to that of silibinin. Patuletin (Chbhnf-07) and spinacetin (Chbhnf-08) triglycosides showed the most protective effects at the lowest concentration of 50 µM.https://pharmacia.pensoft.net/article/71030/download/pdf/ |
| spellingShingle | Zlatina Kokanova-Nedialkova Denitsa Aluani Virginia Tzankova Paraskev Nedialkov Simultaneous quantification of the major flavonoids from wild spinach by UHPLC-HRMS and their neuroprotective effects in a model of H2O2-induced oxidative stress on SH-SY5Y cells Pharmacia |
| title | Simultaneous quantification of the major flavonoids from wild spinach by UHPLC-HRMS and their neuroprotective effects in a model of H2O2-induced oxidative stress on SH-SY5Y cells |
| title_full | Simultaneous quantification of the major flavonoids from wild spinach by UHPLC-HRMS and their neuroprotective effects in a model of H2O2-induced oxidative stress on SH-SY5Y cells |
| title_fullStr | Simultaneous quantification of the major flavonoids from wild spinach by UHPLC-HRMS and their neuroprotective effects in a model of H2O2-induced oxidative stress on SH-SY5Y cells |
| title_full_unstemmed | Simultaneous quantification of the major flavonoids from wild spinach by UHPLC-HRMS and their neuroprotective effects in a model of H2O2-induced oxidative stress on SH-SY5Y cells |
| title_short | Simultaneous quantification of the major flavonoids from wild spinach by UHPLC-HRMS and their neuroprotective effects in a model of H2O2-induced oxidative stress on SH-SY5Y cells |
| title_sort | simultaneous quantification of the major flavonoids from wild spinach by uhplc hrms and their neuroprotective effects in a model of h2o2 induced oxidative stress on sh sy5y cells |
| url | https://pharmacia.pensoft.net/article/71030/download/pdf/ |
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