Analysis of Transcriptomic Differences in the Ovaries of High- and Low-Laying Ducks
The egg-laying performance of Shan Ma ducks (Anas Platyrhynchos) is a crucial economic trait. Nevertheless, limited research has been conducted on the egg-laying performance of this species. We examined routine blood indicators and observed higher levels of metabolic and immune-related factors in th...
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| Format: | Article |
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MDPI AG
2024-01-01
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| Online Access: | https://www.mdpi.com/2073-4425/15/2/181 |
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| author | Yuguang Chang Rongbing Guo Tao Zeng Hanxue Sun Yong Tian Xue Han Yongqing Cao Ligen Xu Mingcai Duan Lizhi Lu Li Chen |
| author_facet | Yuguang Chang Rongbing Guo Tao Zeng Hanxue Sun Yong Tian Xue Han Yongqing Cao Ligen Xu Mingcai Duan Lizhi Lu Li Chen |
| author_sort | Yuguang Chang |
| collection | DOAJ |
| description | The egg-laying performance of Shan Ma ducks (Anas Platyrhynchos) is a crucial economic trait. Nevertheless, limited research has been conducted on the egg-laying performance of this species. We examined routine blood indicators and observed higher levels of metabolic and immune-related factors in the high-egg-production group compared with the low-egg-production group. Furthermore, we explored the ovarian transcriptome of both high- and low-egg-production groups of Shan Ma ducks using Illumina NovaSeq 6000 sequencing. A total of 1357 differentially expressed genes (DEGs) were identified, with 686 down-regulated and 671 up-regulated in the high-egg-production (HEP) ducks and low-egg-production (LEP) ducks. Several genes involved in the regulation of ovarian development, including neuropeptide Y (NPY), cell cycle protein-dependent kinase 1 (CDK1), and transcription factor 1 (E2F1), exhibited significant differential expressions at varying stages of egg production. Pathway functional analysis revealed that the DEGs were primarily associated with the steroid biosynthesis pathway, and the neuroactive ligand–receptor interaction pathway exhibited higher activity in the HEP group compared to the LEP group. This study offers valuable information about and novel insights into high egg production. |
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| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-07T22:31:40Z |
| publishDate | 2024-01-01 |
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| spelling | doaj.art-7212ac5d7b944c40b2076b3193f081172024-02-23T15:17:44ZengMDPI AGGenes2073-44252024-01-0115218110.3390/genes15020181Analysis of Transcriptomic Differences in the Ovaries of High- and Low-Laying DucksYuguang Chang0Rongbing Guo1Tao Zeng2Hanxue Sun3Yong Tian4Xue Han5Yongqing Cao6Ligen Xu7Mingcai Duan8Lizhi Lu9Li Chen10State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Animal Science & Veterinary, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaCollege of Animal Sciences and Veterinary Medicine, Zhejiang A&F University, Hangzhou 311300, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Animal Science & Veterinary, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Animal Science & Veterinary, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Animal Science & Veterinary, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaInstitute of Animal Husbandry and Veterinary Science, Guizhou Academy of Agricultural Sciences, Guiyang 550000, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Animal Science & Veterinary, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Animal Science & Veterinary, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Animal Science & Veterinary, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Animal Science & Veterinary, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Animal Science & Veterinary, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaThe egg-laying performance of Shan Ma ducks (Anas Platyrhynchos) is a crucial economic trait. Nevertheless, limited research has been conducted on the egg-laying performance of this species. We examined routine blood indicators and observed higher levels of metabolic and immune-related factors in the high-egg-production group compared with the low-egg-production group. Furthermore, we explored the ovarian transcriptome of both high- and low-egg-production groups of Shan Ma ducks using Illumina NovaSeq 6000 sequencing. A total of 1357 differentially expressed genes (DEGs) were identified, with 686 down-regulated and 671 up-regulated in the high-egg-production (HEP) ducks and low-egg-production (LEP) ducks. Several genes involved in the regulation of ovarian development, including neuropeptide Y (NPY), cell cycle protein-dependent kinase 1 (CDK1), and transcription factor 1 (E2F1), exhibited significant differential expressions at varying stages of egg production. Pathway functional analysis revealed that the DEGs were primarily associated with the steroid biosynthesis pathway, and the neuroactive ligand–receptor interaction pathway exhibited higher activity in the HEP group compared to the LEP group. This study offers valuable information about and novel insights into high egg production.https://www.mdpi.com/2073-4425/15/2/181duckovaryRNA-seqegg production |
| spellingShingle | Yuguang Chang Rongbing Guo Tao Zeng Hanxue Sun Yong Tian Xue Han Yongqing Cao Ligen Xu Mingcai Duan Lizhi Lu Li Chen Analysis of Transcriptomic Differences in the Ovaries of High- and Low-Laying Ducks Genes duck ovary RNA-seq egg production |
| title | Analysis of Transcriptomic Differences in the Ovaries of High- and Low-Laying Ducks |
| title_full | Analysis of Transcriptomic Differences in the Ovaries of High- and Low-Laying Ducks |
| title_fullStr | Analysis of Transcriptomic Differences in the Ovaries of High- and Low-Laying Ducks |
| title_full_unstemmed | Analysis of Transcriptomic Differences in the Ovaries of High- and Low-Laying Ducks |
| title_short | Analysis of Transcriptomic Differences in the Ovaries of High- and Low-Laying Ducks |
| title_sort | analysis of transcriptomic differences in the ovaries of high and low laying ducks |
| topic | duck ovary RNA-seq egg production |
| url | https://www.mdpi.com/2073-4425/15/2/181 |
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