Influence of different carbon sources on in vitro induction of anthocyanin pigments in callus cultures of petunia (Petunia hybrida)
Anthocyanins are naturally occurring compounds that impart color to fruits, flowers, vegetables, and plants. They are probably the most important group of visible plant pigments besides chlorophyll pigments. Apart from imparting color to plants, anthocyanins also have an array of health-promoting b...
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| Format: | Article |
| Language: | English |
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Indian Council of Agricultural Research
2014-08-01
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| Series: | The Indian Journal of Agricultural Sciences |
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| Online Access: | https://epubs.icar.org.in/index.php/IJAgS/article/view/43085 |
| _version_ | 1797692396617596928 |
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| author | USHA T JANAKIRAM K V PRASAD CHARANJIT KAUR P SRINIVASAN C TARA SATYAVATHI |
| author_facet | USHA T JANAKIRAM K V PRASAD CHARANJIT KAUR P SRINIVASAN C TARA SATYAVATHI |
| author_sort | USHA |
| collection | DOAJ |
| description |
Anthocyanins are naturally occurring compounds that impart color to fruits, flowers, vegetables, and plants. They are probably the most important group of visible plant pigments besides chlorophyll pigments. Apart from imparting color to plants, anthocyanins also have an array of health-promoting benefits, as they can protect against a variety of free radicals through a various number of mechanisms. Development of an efficient tissue culture system for commercial production of anthocyanins requires an integrated approach through manipulation of various media constituents. The effect of varied concentrations of different carbon sources on anthocyanin production in callus cultures of Petunia hybrida cv Bravo Blue was studied. Explants from young leaves were cultured on Murashige and Skoog (MS) medium supplemented with MS + IBA (19.6 µM) + Kin. (4.65 µM) + AdS (81.45 mM), 3% sucrose and 0.7% agar. Among the various carbon sources tested, incorporation of Glucose at 5% was found to have earliest pigment induction with maximum response coefficient with highest pigment content (1.36 ± 0.012 CV/g FCW). Highest gain in fresh cell weight was noticed with the addition of sucrose 5% (3.96 ± 0.06 g). When MS medium was supplemented with different concentrations of Galactose, the explants failed to respond.
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| first_indexed | 2024-03-12T02:27:03Z |
| format | Article |
| id | doaj.art-7213f45fed0e4991aa9919d5d53cc59a |
| institution | Directory Open Access Journal |
| issn | 0019-5022 2394-3319 |
| language | English |
| last_indexed | 2024-03-12T02:27:03Z |
| publishDate | 2014-08-01 |
| publisher | Indian Council of Agricultural Research |
| record_format | Article |
| series | The Indian Journal of Agricultural Sciences |
| spelling | doaj.art-7213f45fed0e4991aa9919d5d53cc59a2023-09-05T11:38:31ZengIndian Council of Agricultural ResearchThe Indian Journal of Agricultural Sciences0019-50222394-33192014-08-0184810.56093/ijas.v84i8.43085Influence of different carbon sources on in vitro induction of anthocyanin pigments in callus cultures of petunia (Petunia hybrida)USHA0T JANAKIRAM1K V PRASAD2CHARANJIT KAUR3P SRINIVASAN4C TARA SATYAVATHI5Indian Agricultural Research Institute, New Delhi 110 012Indian Agricultural Research Institute, New Delhi 110 012Indian Agricultural Research Institute, New Delhi 110 012Indian Agricultural Research Institute, New Delhi 110 012Indian Agricultural Research Institute, New Delhi 110 012Indian Agricultural Research Institute, New Delhi 110 012 Anthocyanins are naturally occurring compounds that impart color to fruits, flowers, vegetables, and plants. They are probably the most important group of visible plant pigments besides chlorophyll pigments. Apart from imparting color to plants, anthocyanins also have an array of health-promoting benefits, as they can protect against a variety of free radicals through a various number of mechanisms. Development of an efficient tissue culture system for commercial production of anthocyanins requires an integrated approach through manipulation of various media constituents. The effect of varied concentrations of different carbon sources on anthocyanin production in callus cultures of Petunia hybrida cv Bravo Blue was studied. Explants from young leaves were cultured on Murashige and Skoog (MS) medium supplemented with MS + IBA (19.6 µM) + Kin. (4.65 µM) + AdS (81.45 mM), 3% sucrose and 0.7% agar. Among the various carbon sources tested, incorporation of Glucose at 5% was found to have earliest pigment induction with maximum response coefficient with highest pigment content (1.36 ± 0.012 CV/g FCW). Highest gain in fresh cell weight was noticed with the addition of sucrose 5% (3.96 ± 0.06 g). When MS medium was supplemented with different concentrations of Galactose, the explants failed to respond. https://epubs.icar.org.in/index.php/IJAgS/article/view/43085AnthocyaninCallus culturesColour value indexFructoseGalactoseGlucose |
| spellingShingle | USHA T JANAKIRAM K V PRASAD CHARANJIT KAUR P SRINIVASAN C TARA SATYAVATHI Influence of different carbon sources on in vitro induction of anthocyanin pigments in callus cultures of petunia (Petunia hybrida) The Indian Journal of Agricultural Sciences Anthocyanin Callus cultures Colour value index Fructose Galactose Glucose |
| title | Influence of different carbon sources on in vitro induction of anthocyanin pigments in callus cultures of petunia (Petunia hybrida) |
| title_full | Influence of different carbon sources on in vitro induction of anthocyanin pigments in callus cultures of petunia (Petunia hybrida) |
| title_fullStr | Influence of different carbon sources on in vitro induction of anthocyanin pigments in callus cultures of petunia (Petunia hybrida) |
| title_full_unstemmed | Influence of different carbon sources on in vitro induction of anthocyanin pigments in callus cultures of petunia (Petunia hybrida) |
| title_short | Influence of different carbon sources on in vitro induction of anthocyanin pigments in callus cultures of petunia (Petunia hybrida) |
| title_sort | influence of different carbon sources on in vitro induction of anthocyanin pigments in callus cultures of petunia petunia hybrida |
| topic | Anthocyanin Callus cultures Colour value index Fructose Galactose Glucose |
| url | https://epubs.icar.org.in/index.php/IJAgS/article/view/43085 |
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