Presentation of a novel model of chitosan- polyethylene oxide-nanohydroxyapatite nanofibers together with bone marrow stromal cells to repair and improve minor bone defects
Objective(s):Various methods for repairing bone defects are presented. Cell therapy is one of these methods. Bone marrow stromal cells (BMSCs) seem to be suitable for this purpose. On the other hand, lots of biomaterials are used to improve and repair the defect in the body, so in this study we trie...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2015-09-01
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| Series: | Iranian Journal of Basic Medical Sciences |
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| Online Access: | http://ijbms.mums.ac.ir/pdf_5211_cc95a446fa3de7d71c270924ffc86348.html |
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| author | Asgar Emamgholi Mohsen Rahimi Gholamreza Kaka Seyed Homayoon Sadraie Saleh Najafi |
| author_facet | Asgar Emamgholi Mohsen Rahimi Gholamreza Kaka Seyed Homayoon Sadraie Saleh Najafi |
| author_sort | Asgar Emamgholi |
| collection | DOAJ |
| description | Objective(s):Various methods for repairing bone defects are presented. Cell therapy is one of these methods. Bone marrow stromal cells (BMSCs) seem to be suitable for this purpose. On the other hand, lots of biomaterials are used to improve and repair the defect in the body, so in this study we tried to produce a similar structure to the bone by the chitosan and hydroxyapatite. Materials and Methods: In this study, the solution of chitosan-nanohydroxyapatite-polyethylene oxide (PEO) Nanofibers was produced by electrospinning method, and then the BMSCs were cultured on this solution. A piece of chitosan-nanohydroxyapatite Nanofibers with BMSCs was placed in a hole with the diameter of 1 mm at the distal epiphysis of the rat femur. Then the biomechanical and radiographic studies were performed. Results: Biomechanical testing results showed that bone strength was significantly higher in the Nanofiber/BMSCs group in comparison with control group. Also the bone strength in nanofiber/BMSCs group was significant, but in nanofiber group was nearly significant. Radiographic studies also showed that the average amount of callus formation (radio opacity) in nanofiber and control group was not significantly different. The callus formation in nanofiber/BMSCs group was increased compared to the control group, and it was not significant in the nanofiber group. Conclusion: Since chitosan-nanohydroxyapatite nanofibers with BMSCs increases the rate of bone repair, the obtained cell-nanoscaffold shell can be used in tissue engineering and cell therapy, especially for bone defects. |
| first_indexed | 2024-12-21T05:39:46Z |
| format | Article |
| id | doaj.art-721c74853ec146a48051e0c7d91d7a9c |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-12-21T05:39:46Z |
| publishDate | 2015-09-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-721c74853ec146a48051e0c7d91d7a9c2022-12-21T19:14:17ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742015-09-011898878935211Presentation of a novel model of chitosan- polyethylene oxide-nanohydroxyapatite nanofibers together with bone marrow stromal cells to repair and improve minor bone defectsAsgar Emamgholi0Mohsen Rahimi1Gholamreza Kaka2Seyed Homayoon Sadraie3Saleh Najafi4Neuroscience Research Center, Baqiyatallah University of Medical Science, Tehran, Iran|Microbial Biotechnology, Faculty of Science, Payame Noor University, Unit of Tehran Center, Tehran, IranDepartment of Parasitology and Mycology, School of Medicine, Baqiyatallah University of Medical Science, Tehran, IranNeuroscience Research Center, Baqiyatallah University of Medical Science, Tehran, IranDepartment of Anatomy, School of Medicine, Baqiyatallah University of Medical Science, Tehran, IranMicrobial Biotechnology, Faculty of Science, Payame Noor University, Unit of Tehran Center, Tehran, IranObjective(s):Various methods for repairing bone defects are presented. Cell therapy is one of these methods. Bone marrow stromal cells (BMSCs) seem to be suitable for this purpose. On the other hand, lots of biomaterials are used to improve and repair the defect in the body, so in this study we tried to produce a similar structure to the bone by the chitosan and hydroxyapatite. Materials and Methods: In this study, the solution of chitosan-nanohydroxyapatite-polyethylene oxide (PEO) Nanofibers was produced by electrospinning method, and then the BMSCs were cultured on this solution. A piece of chitosan-nanohydroxyapatite Nanofibers with BMSCs was placed in a hole with the diameter of 1 mm at the distal epiphysis of the rat femur. Then the biomechanical and radiographic studies were performed. Results: Biomechanical testing results showed that bone strength was significantly higher in the Nanofiber/BMSCs group in comparison with control group. Also the bone strength in nanofiber/BMSCs group was significant, but in nanofiber group was nearly significant. Radiographic studies also showed that the average amount of callus formation (radio opacity) in nanofiber and control group was not significantly different. The callus formation in nanofiber/BMSCs group was increased compared to the control group, and it was not significant in the nanofiber group. Conclusion: Since chitosan-nanohydroxyapatite nanofibers with BMSCs increases the rate of bone repair, the obtained cell-nanoscaffold shell can be used in tissue engineering and cell therapy, especially for bone defects.http://ijbms.mums.ac.ir/pdf_5211_cc95a446fa3de7d71c270924ffc86348.htmlBMSCsChitosanNanofibersScaffolds |
| spellingShingle | Asgar Emamgholi Mohsen Rahimi Gholamreza Kaka Seyed Homayoon Sadraie Saleh Najafi Presentation of a novel model of chitosan- polyethylene oxide-nanohydroxyapatite nanofibers together with bone marrow stromal cells to repair and improve minor bone defects Iranian Journal of Basic Medical Sciences BMSCs Chitosan Nanofibers Scaffolds |
| title | Presentation of a novel model of chitosan- polyethylene oxide-nanohydroxyapatite nanofibers together with bone marrow stromal cells to repair and improve minor bone defects |
| title_full | Presentation of a novel model of chitosan- polyethylene oxide-nanohydroxyapatite nanofibers together with bone marrow stromal cells to repair and improve minor bone defects |
| title_fullStr | Presentation of a novel model of chitosan- polyethylene oxide-nanohydroxyapatite nanofibers together with bone marrow stromal cells to repair and improve minor bone defects |
| title_full_unstemmed | Presentation of a novel model of chitosan- polyethylene oxide-nanohydroxyapatite nanofibers together with bone marrow stromal cells to repair and improve minor bone defects |
| title_short | Presentation of a novel model of chitosan- polyethylene oxide-nanohydroxyapatite nanofibers together with bone marrow stromal cells to repair and improve minor bone defects |
| title_sort | presentation of a novel model of chitosan polyethylene oxide nanohydroxyapatite nanofibers together with bone marrow stromal cells to repair and improve minor bone defects |
| topic | BMSCs Chitosan Nanofibers Scaffolds |
| url | http://ijbms.mums.ac.ir/pdf_5211_cc95a446fa3de7d71c270924ffc86348.html |
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