Motility Subpopulations with Distinct Motility Characteristics Using Swim-Up-Selected Sperm Cells from Norwegian Red Bulls: Effects of Freezing–Thawing and Between-Bull Variation
Discrete subpopulations of motile sperm cells have been found for several species and are implicated to be important for sperm functionality. The aim of this present study was to examine the motile subpopulations in swim-up-selected bull spermatozoa and the relationship between subpopulations in fre...
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| Format: | Article |
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MDPI AG
2023-08-01
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| Series: | Biology |
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| Online Access: | https://www.mdpi.com/2079-7737/12/8/1086 |
| _version_ | 1797585459487965184 |
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| author | Anne Hege Alm-Kristiansen |
| author_facet | Anne Hege Alm-Kristiansen |
| author_sort | Anne Hege Alm-Kristiansen |
| collection | DOAJ |
| description | Discrete subpopulations of motile sperm cells have been found for several species and are implicated to be important for sperm functionality. The aim of this present study was to examine the motile subpopulations in swim-up-selected bull spermatozoa and the relationship between subpopulations in fresh and frozen–thawed sperm cells. In experiment 1, swim-up (SWUP)-selected and non-selected (control) sperm cells were analyzed using a Computer-Assisted Sperm Analyzer (CASA). In experiment 2, the semen from nine bulls was cryopreserved and analyzed using CASA both before and after freezing and after incubation at physiological temperatures. The SWUP population had a higher proportion of total motility, progressivity, and velocity compared to the control (<i>p</i> < 0.05). Likewise, both incubation over time and cryopreservation affected motility and motility parameters (<i>p</i> < 0.05). The population of rapid progressive (RapidP) sperm cells dominated the SWUP fraction and was higher than in the control samples (<i>p</i> < 0.05). Furthermore, RapidP was also the main part of fresh semen, but decreased significantly over time during incubation and due to cryopreservation. In conclusion, RapidP was the main population in SWUP-selected spermatozoa and seems to be an important subpopulation contributing to the differences between treatments and in response to the freezing of sperm cells. |
| first_indexed | 2024-03-11T00:06:32Z |
| format | Article |
| id | doaj.art-722120618dd64b5aa1982f046357acb7 |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-11T00:06:32Z |
| publishDate | 2023-08-01 |
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| spelling | doaj.art-722120618dd64b5aa1982f046357acb72023-11-19T00:18:55ZengMDPI AGBiology2079-77372023-08-01128108610.3390/biology12081086Motility Subpopulations with Distinct Motility Characteristics Using Swim-Up-Selected Sperm Cells from Norwegian Red Bulls: Effects of Freezing–Thawing and Between-Bull VariationAnne Hege Alm-Kristiansen0Faculty of Applied Ecology, Agricultural Sciences and Biotechnology, Inland Norway University of Applied Sciences, 2418 Hamar, NorwayDiscrete subpopulations of motile sperm cells have been found for several species and are implicated to be important for sperm functionality. The aim of this present study was to examine the motile subpopulations in swim-up-selected bull spermatozoa and the relationship between subpopulations in fresh and frozen–thawed sperm cells. In experiment 1, swim-up (SWUP)-selected and non-selected (control) sperm cells were analyzed using a Computer-Assisted Sperm Analyzer (CASA). In experiment 2, the semen from nine bulls was cryopreserved and analyzed using CASA both before and after freezing and after incubation at physiological temperatures. The SWUP population had a higher proportion of total motility, progressivity, and velocity compared to the control (<i>p</i> < 0.05). Likewise, both incubation over time and cryopreservation affected motility and motility parameters (<i>p</i> < 0.05). The population of rapid progressive (RapidP) sperm cells dominated the SWUP fraction and was higher than in the control samples (<i>p</i> < 0.05). Furthermore, RapidP was also the main part of fresh semen, but decreased significantly over time during incubation and due to cryopreservation. In conclusion, RapidP was the main population in SWUP-selected spermatozoa and seems to be an important subpopulation contributing to the differences between treatments and in response to the freezing of sperm cells.https://www.mdpi.com/2079-7737/12/8/1086bovinesperm selectioncryopreservationCASA |
| spellingShingle | Anne Hege Alm-Kristiansen Motility Subpopulations with Distinct Motility Characteristics Using Swim-Up-Selected Sperm Cells from Norwegian Red Bulls: Effects of Freezing–Thawing and Between-Bull Variation Biology bovine sperm selection cryopreservation CASA |
| title | Motility Subpopulations with Distinct Motility Characteristics Using Swim-Up-Selected Sperm Cells from Norwegian Red Bulls: Effects of Freezing–Thawing and Between-Bull Variation |
| title_full | Motility Subpopulations with Distinct Motility Characteristics Using Swim-Up-Selected Sperm Cells from Norwegian Red Bulls: Effects of Freezing–Thawing and Between-Bull Variation |
| title_fullStr | Motility Subpopulations with Distinct Motility Characteristics Using Swim-Up-Selected Sperm Cells from Norwegian Red Bulls: Effects of Freezing–Thawing and Between-Bull Variation |
| title_full_unstemmed | Motility Subpopulations with Distinct Motility Characteristics Using Swim-Up-Selected Sperm Cells from Norwegian Red Bulls: Effects of Freezing–Thawing and Between-Bull Variation |
| title_short | Motility Subpopulations with Distinct Motility Characteristics Using Swim-Up-Selected Sperm Cells from Norwegian Red Bulls: Effects of Freezing–Thawing and Between-Bull Variation |
| title_sort | motility subpopulations with distinct motility characteristics using swim up selected sperm cells from norwegian red bulls effects of freezing thawing and between bull variation |
| topic | bovine sperm selection cryopreservation CASA |
| url | https://www.mdpi.com/2079-7737/12/8/1086 |
| work_keys_str_mv | AT annehegealmkristiansen motilitysubpopulationswithdistinctmotilitycharacteristicsusingswimupselectedspermcellsfromnorwegianredbullseffectsoffreezingthawingandbetweenbullvariation |