Subunits of the PBAP Chromatin Remodeler Are Capable of Mediating Enhancer-Driven Transcription in <i>Drosophila</i>
The chromatin remodeler SWI/SNF is an important participant in gene activation, functioning predominantly by opening the chromatin structure on promoters and enhancers. Here, we describe its novel mode of action in which SWI/SNF factors mediate the targeted action of an enhancer. We studied the func...
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| Format: | Article |
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MDPI AG
2021-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/6/2856 |
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| author | Yulii V. Shidlovskii Oleg V. Bylino Alexander V. Shaposhnikov Zaur M. Kachaev Lyubov A. Lebedeva Valeria V. Kolesnik Diego Amendola Giovanna De Simone Nadia Formicola Paul Schedl Filomena Anna Digilio Ennio Giordano |
| author_facet | Yulii V. Shidlovskii Oleg V. Bylino Alexander V. Shaposhnikov Zaur M. Kachaev Lyubov A. Lebedeva Valeria V. Kolesnik Diego Amendola Giovanna De Simone Nadia Formicola Paul Schedl Filomena Anna Digilio Ennio Giordano |
| author_sort | Yulii V. Shidlovskii |
| collection | DOAJ |
| description | The chromatin remodeler SWI/SNF is an important participant in gene activation, functioning predominantly by opening the chromatin structure on promoters and enhancers. Here, we describe its novel mode of action in which SWI/SNF factors mediate the targeted action of an enhancer. We studied the functions of two signature subunits of PBAP subfamily, BAP170 and SAYP, in <i>Drosophila</i>. These subunits were stably tethered to a transgene reporter carrying the <i>hsp70</i> core promoter. The tethered subunits mediate transcription of the reporter in a pattern that is generated by enhancers close to the insertion site in multiple loci throughout the genome. Both tethered SAYP and BAP170 recruit the whole PBAP complex to the reporter promoter. However, we found that BAP170-dependent transcription is more resistant to the depletion of other PBAP subunits, suggesting that BAP170 may play a more critical role in establishing enhancer-dependent transcription. |
| first_indexed | 2024-03-10T13:20:21Z |
| format | Article |
| id | doaj.art-7224c65326a74899804ff395cc94e64f |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T13:20:21Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-7224c65326a74899804ff395cc94e64f2023-11-21T10:05:35ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-03-01226285610.3390/ijms22062856Subunits of the PBAP Chromatin Remodeler Are Capable of Mediating Enhancer-Driven Transcription in <i>Drosophila</i>Yulii V. Shidlovskii0Oleg V. Bylino1Alexander V. Shaposhnikov2Zaur M. Kachaev3Lyubov A. Lebedeva4Valeria V. Kolesnik5Diego Amendola6Giovanna De Simone7Nadia Formicola8Paul Schedl9Filomena Anna Digilio10Ennio Giordano11Department of Gene Expression Regulation in Development, Institute of Gene Biology, Russian Academy of Sciences, 119334 Moscow, RussiaDepartment of Gene Expression Regulation in Development, Institute of Gene Biology, Russian Academy of Sciences, 119334 Moscow, RussiaDepartment of Gene Expression Regulation in Development, Institute of Gene Biology, Russian Academy of Sciences, 119334 Moscow, RussiaDepartment of Gene Expression Regulation in Development, Institute of Gene Biology, Russian Academy of Sciences, 119334 Moscow, RussiaDepartment of Gene Expression Regulation in Development, Institute of Gene Biology, Russian Academy of Sciences, 119334 Moscow, RussiaDepartment of Gene Expression Regulation in Development, Institute of Gene Biology, Russian Academy of Sciences, 119334 Moscow, RussiaDepartment of Biology, Università di Napoli Federico II, 80138 Naples, ItalyDepartment of Biology, Università di Napoli Federico II, 80138 Naples, ItalyInstitute of Research on Terrestrial Ecosystems (IRET) National Research Council (CNR), 05010 Porano, ItalyDepartment of Gene Expression Regulation in Development, Institute of Gene Biology, Russian Academy of Sciences, 119334 Moscow, RussiaInstitute of Research on Terrestrial Ecosystems (IRET) National Research Council (CNR), 05010 Porano, ItalyDepartment of Biology, Università di Napoli Federico II, 80138 Naples, ItalyThe chromatin remodeler SWI/SNF is an important participant in gene activation, functioning predominantly by opening the chromatin structure on promoters and enhancers. Here, we describe its novel mode of action in which SWI/SNF factors mediate the targeted action of an enhancer. We studied the functions of two signature subunits of PBAP subfamily, BAP170 and SAYP, in <i>Drosophila</i>. These subunits were stably tethered to a transgene reporter carrying the <i>hsp70</i> core promoter. The tethered subunits mediate transcription of the reporter in a pattern that is generated by enhancers close to the insertion site in multiple loci throughout the genome. Both tethered SAYP and BAP170 recruit the whole PBAP complex to the reporter promoter. However, we found that BAP170-dependent transcription is more resistant to the depletion of other PBAP subunits, suggesting that BAP170 may play a more critical role in establishing enhancer-dependent transcription.https://www.mdpi.com/1422-0067/22/6/2856SWI/SNFPBAPchromatin remodelingpromoterenhancer |
| spellingShingle | Yulii V. Shidlovskii Oleg V. Bylino Alexander V. Shaposhnikov Zaur M. Kachaev Lyubov A. Lebedeva Valeria V. Kolesnik Diego Amendola Giovanna De Simone Nadia Formicola Paul Schedl Filomena Anna Digilio Ennio Giordano Subunits of the PBAP Chromatin Remodeler Are Capable of Mediating Enhancer-Driven Transcription in <i>Drosophila</i> International Journal of Molecular Sciences SWI/SNF PBAP chromatin remodeling promoter enhancer |
| title | Subunits of the PBAP Chromatin Remodeler Are Capable of Mediating Enhancer-Driven Transcription in <i>Drosophila</i> |
| title_full | Subunits of the PBAP Chromatin Remodeler Are Capable of Mediating Enhancer-Driven Transcription in <i>Drosophila</i> |
| title_fullStr | Subunits of the PBAP Chromatin Remodeler Are Capable of Mediating Enhancer-Driven Transcription in <i>Drosophila</i> |
| title_full_unstemmed | Subunits of the PBAP Chromatin Remodeler Are Capable of Mediating Enhancer-Driven Transcription in <i>Drosophila</i> |
| title_short | Subunits of the PBAP Chromatin Remodeler Are Capable of Mediating Enhancer-Driven Transcription in <i>Drosophila</i> |
| title_sort | subunits of the pbap chromatin remodeler are capable of mediating enhancer driven transcription in i drosophila i |
| topic | SWI/SNF PBAP chromatin remodeling promoter enhancer |
| url | https://www.mdpi.com/1422-0067/22/6/2856 |
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