Development and Validation of the LC–MS/MS Method for Determination of 130 Natural and Synthetic Cannabinoids in Cannabis Oil
Dietary supplements are widely available products used by millions of people around the world. Unfortunately, the procedure of adding pharmaceutical and psychoactive substances has recently been observed, in order to increase the effectiveness of supplements in the form of hemp oils. For this reason...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/23/8601 |
| _version_ | 1797462587220164608 |
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| author | Natalia Galant Jakub Czarny Jolanta Powierska-Czarny Agnieszka Piotrowska-Cyplik |
| author_facet | Natalia Galant Jakub Czarny Jolanta Powierska-Czarny Agnieszka Piotrowska-Cyplik |
| author_sort | Natalia Galant |
| collection | DOAJ |
| description | Dietary supplements are widely available products used by millions of people around the world. Unfortunately, the procedure of adding pharmaceutical and psychoactive substances has recently been observed, in order to increase the effectiveness of supplements in the form of hemp oils. For this reason, it is extremely important to develop analytical methods for the detection of substances prohibited in dietary supplements and food products. In the present study, using the LC–MS/MS technique, an innovative method for the detection and quantification of 117 synthetic cannabinoids and 13 natural cannabinoids in dietary supplements and food products in the form of oils during one 13-min chromatographic run was developed. Each method was fully validated by characterization of the following parameters: The limit of detection was set to 0.1 ng/mL (100 µg/g, 0.01%). The limit of quantification ranged from 0.05 ng/mL to 50 ng/mL. The criteria assumed for systematic error caused by methodological bias (±20%) resulting from the recovery of analytes after the extraction process, as well as the coefficient of variation (CV) (≤20%), were met for all 130 tested compounds. The positive results of the validation confirmed that the developed methods met the requirements related to the adequacy of their application in a given scope. Additionally, methods developed using the LC–MS/MS technique were verified via proficiency tests. The developed analytical procedure was successfully used in the analysis of hemp oils and capsules containing them in the studied dietary supplements. |
| first_indexed | 2024-03-09T17:38:43Z |
| format | Article |
| id | doaj.art-722933ff1684484b9c932183c90cb61c |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T17:38:43Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-722933ff1684484b9c932183c90cb61c2023-11-24T11:45:00ZengMDPI AGMolecules1420-30492022-12-012723860110.3390/molecules27238601Development and Validation of the LC–MS/MS Method for Determination of 130 Natural and Synthetic Cannabinoids in Cannabis OilNatalia Galant0Jakub Czarny1Jolanta Powierska-Czarny2Agnieszka Piotrowska-Cyplik3Institute of Forensic Genetics, Al. Mickiewicza 3/4, 85-071 Bydgoszcz, PolandInstitute of Forensic Genetics, Al. Mickiewicza 3/4, 85-071 Bydgoszcz, PolandInstitute of Forensic Genetics, Al. Mickiewicza 3/4, 85-071 Bydgoszcz, PolandDepartment of Food Technology of Plant Origin, Poznan University of Life Sciences, Wojska Polskiego 31, 60-624 Poznan, PolandDietary supplements are widely available products used by millions of people around the world. Unfortunately, the procedure of adding pharmaceutical and psychoactive substances has recently been observed, in order to increase the effectiveness of supplements in the form of hemp oils. For this reason, it is extremely important to develop analytical methods for the detection of substances prohibited in dietary supplements and food products. In the present study, using the LC–MS/MS technique, an innovative method for the detection and quantification of 117 synthetic cannabinoids and 13 natural cannabinoids in dietary supplements and food products in the form of oils during one 13-min chromatographic run was developed. Each method was fully validated by characterization of the following parameters: The limit of detection was set to 0.1 ng/mL (100 µg/g, 0.01%). The limit of quantification ranged from 0.05 ng/mL to 50 ng/mL. The criteria assumed for systematic error caused by methodological bias (±20%) resulting from the recovery of analytes after the extraction process, as well as the coefficient of variation (CV) (≤20%), were met for all 130 tested compounds. The positive results of the validation confirmed that the developed methods met the requirements related to the adequacy of their application in a given scope. Additionally, methods developed using the LC–MS/MS technique were verified via proficiency tests. The developed analytical procedure was successfully used in the analysis of hemp oils and capsules containing them in the studied dietary supplements.https://www.mdpi.com/1420-3049/27/23/8601cannabinoidscannabis oildietary supplementsLC–MS/MS |
| spellingShingle | Natalia Galant Jakub Czarny Jolanta Powierska-Czarny Agnieszka Piotrowska-Cyplik Development and Validation of the LC–MS/MS Method for Determination of 130 Natural and Synthetic Cannabinoids in Cannabis Oil Molecules cannabinoids cannabis oil dietary supplements LC–MS/MS |
| title | Development and Validation of the LC–MS/MS Method for Determination of 130 Natural and Synthetic Cannabinoids in Cannabis Oil |
| title_full | Development and Validation of the LC–MS/MS Method for Determination of 130 Natural and Synthetic Cannabinoids in Cannabis Oil |
| title_fullStr | Development and Validation of the LC–MS/MS Method for Determination of 130 Natural and Synthetic Cannabinoids in Cannabis Oil |
| title_full_unstemmed | Development and Validation of the LC–MS/MS Method for Determination of 130 Natural and Synthetic Cannabinoids in Cannabis Oil |
| title_short | Development and Validation of the LC–MS/MS Method for Determination of 130 Natural and Synthetic Cannabinoids in Cannabis Oil |
| title_sort | development and validation of the lc ms ms method for determination of 130 natural and synthetic cannabinoids in cannabis oil |
| topic | cannabinoids cannabis oil dietary supplements LC–MS/MS |
| url | https://www.mdpi.com/1420-3049/27/23/8601 |
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