Evaluating the association between feed efficiency and the fecal microbiota of early-life Duroc pigs using 16S rRNA sequencing
Abstract Improving the predication efficiency of porcine production performance at early stage will contribute to reducing the breeding and production costs. The intestinal microbiota had received plenty of attention in recent years due to their influence on host health and performance. The purpose...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2020-06-01
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| Series: | AMB Express |
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| Online Access: | http://link.springer.com/article/10.1186/s13568-020-01050-2 |
| _version_ | 1818936283542585344 |
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| author | Jinglei Si Lingli Feng Jiuyu Gao Ye Huang Guangjie Zhang Jiayuan Mo Siran Zhu Wenjing Qi Jing Liang Ganqiu Lan |
| author_facet | Jinglei Si Lingli Feng Jiuyu Gao Ye Huang Guangjie Zhang Jiayuan Mo Siran Zhu Wenjing Qi Jing Liang Ganqiu Lan |
| author_sort | Jinglei Si |
| collection | DOAJ |
| description | Abstract Improving the predication efficiency of porcine production performance at early stage will contribute to reducing the breeding and production costs. The intestinal microbiota had received plenty of attention in recent years due to their influence on host health and performance. The purpose of this study was to investigate the relationship between the fecal microbiota at early growth period and porcine feed efficiency (FE) under a commercial feeding environment. Ninety-one pigs were reordered according to the residual feed intake (RFI) values between day 90 on test and day 160 off test, 9 lowest RFI pigs and 9 highest RFI pigs were selected as the LRFI group and the HRFI group, respectively. Fecal samples from pigs in the early grower phase (day 80) were performed for microbial diversity, composition, and predicted functionality by using 16S rRNA sequencing. The results showed that no significant differences in microbial alpha diversity were observed between two RFI groups, whereas, some RFI-associated compositional differences were revealed. In particular, the microbiota of the LRFI group (more feed-efficient) had significantly higher levels of some members of Clostridiales and Bacteroidales (e.g., g_1_68 and g_norank_f_p_2534_18B5), which may promoted FE through protecting gut barrier function, compared with those of the HRFI pigs. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways analysis found that the LRFI pigs were likely have microbiota with higher levels of amino acid metabolism. Moreover, redundancy analysis (RDA) showed that litter size, parity, and date of birth had significant effects on the bacterial community structure. These results improved our knowledge of the porcine early-life fecal microbiota and its potential link underlying RFI, which would be useful for future development of microbial biomarkers for predicting and improving porcine FE as well as investigation of targets for dietary strategies. |
| first_indexed | 2024-12-20T05:33:36Z |
| format | Article |
| id | doaj.art-722ae704594b462cb17ddb9bb45049a6 |
| institution | Directory Open Access Journal |
| issn | 2191-0855 |
| language | English |
| last_indexed | 2024-12-20T05:33:36Z |
| publishDate | 2020-06-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | AMB Express |
| spelling | doaj.art-722ae704594b462cb17ddb9bb45049a62022-12-21T19:51:41ZengSpringerOpenAMB Express2191-08552020-06-0110111110.1186/s13568-020-01050-2Evaluating the association between feed efficiency and the fecal microbiota of early-life Duroc pigs using 16S rRNA sequencingJinglei Si0Lingli Feng1Jiuyu Gao2Ye Huang3Guangjie Zhang4Jiayuan Mo5Siran Zhu6Wenjing Qi7Jing Liang8Ganqiu Lan9College of Animal Science & Technology, Guangxi UniversityCollege of Animal Science & Technology, Guangxi UniversityCollege of Animal Science & Technology, Guangxi UniversityCollege of Animal Science & Technology, Guangxi UniversityCollege of Animal Science & Technology, Guangxi UniversityCollege of Animal Science & Technology, Guangxi UniversityCollege of Animal Science & Technology, Guangxi UniversityCollege of Animal Science & Technology, Guangxi UniversityCollege of Animal Science & Technology, Guangxi UniversityCollege of Animal Science & Technology, Guangxi UniversityAbstract Improving the predication efficiency of porcine production performance at early stage will contribute to reducing the breeding and production costs. The intestinal microbiota had received plenty of attention in recent years due to their influence on host health and performance. The purpose of this study was to investigate the relationship between the fecal microbiota at early growth period and porcine feed efficiency (FE) under a commercial feeding environment. Ninety-one pigs were reordered according to the residual feed intake (RFI) values between day 90 on test and day 160 off test, 9 lowest RFI pigs and 9 highest RFI pigs were selected as the LRFI group and the HRFI group, respectively. Fecal samples from pigs in the early grower phase (day 80) were performed for microbial diversity, composition, and predicted functionality by using 16S rRNA sequencing. The results showed that no significant differences in microbial alpha diversity were observed between two RFI groups, whereas, some RFI-associated compositional differences were revealed. In particular, the microbiota of the LRFI group (more feed-efficient) had significantly higher levels of some members of Clostridiales and Bacteroidales (e.g., g_1_68 and g_norank_f_p_2534_18B5), which may promoted FE through protecting gut barrier function, compared with those of the HRFI pigs. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways analysis found that the LRFI pigs were likely have microbiota with higher levels of amino acid metabolism. Moreover, redundancy analysis (RDA) showed that litter size, parity, and date of birth had significant effects on the bacterial community structure. These results improved our knowledge of the porcine early-life fecal microbiota and its potential link underlying RFI, which would be useful for future development of microbial biomarkers for predicting and improving porcine FE as well as investigation of targets for dietary strategies.http://link.springer.com/article/10.1186/s13568-020-01050-216S rRNAFecal microbiotaFeed efficiencyHigh-throughput sequencingPigResidual feed intake |
| spellingShingle | Jinglei Si Lingli Feng Jiuyu Gao Ye Huang Guangjie Zhang Jiayuan Mo Siran Zhu Wenjing Qi Jing Liang Ganqiu Lan Evaluating the association between feed efficiency and the fecal microbiota of early-life Duroc pigs using 16S rRNA sequencing AMB Express 16S rRNA Fecal microbiota Feed efficiency High-throughput sequencing Pig Residual feed intake |
| title | Evaluating the association between feed efficiency and the fecal microbiota of early-life Duroc pigs using 16S rRNA sequencing |
| title_full | Evaluating the association between feed efficiency and the fecal microbiota of early-life Duroc pigs using 16S rRNA sequencing |
| title_fullStr | Evaluating the association between feed efficiency and the fecal microbiota of early-life Duroc pigs using 16S rRNA sequencing |
| title_full_unstemmed | Evaluating the association between feed efficiency and the fecal microbiota of early-life Duroc pigs using 16S rRNA sequencing |
| title_short | Evaluating the association between feed efficiency and the fecal microbiota of early-life Duroc pigs using 16S rRNA sequencing |
| title_sort | evaluating the association between feed efficiency and the fecal microbiota of early life duroc pigs using 16s rrna sequencing |
| topic | 16S rRNA Fecal microbiota Feed efficiency High-throughput sequencing Pig Residual feed intake |
| url | http://link.springer.com/article/10.1186/s13568-020-01050-2 |
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