Changes in DNA Methylation and Gene Expression of Insulin and Obesity-Related Gene <i>PIK3R1</i> after Roux-en-Y Gastric Bypass
Weight regulation and the magnitude of weight loss after a Roux-en-Y gastric bypass (RYGB) can be genetically determined. DNA methylation patterns and the expression of some genes can be altered after weight loss interventions, including RYGB. The present study aimed to evaluate how the gene express...
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| Format: | Article |
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MDPI AG
2020-06-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/12/4476 |
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| author | Marcela A S Pinhel Natália Y Noronha Carolina F Nicoletti Vanessa AB Pereira Bruno AP de Oliveira Cristiana Cortes-Oliveira Wilson Salgado Fernando Barbosa Júlio S Marchini Doroteia RS Souza Carla B Nonino |
| author_facet | Marcela A S Pinhel Natália Y Noronha Carolina F Nicoletti Vanessa AB Pereira Bruno AP de Oliveira Cristiana Cortes-Oliveira Wilson Salgado Fernando Barbosa Júlio S Marchini Doroteia RS Souza Carla B Nonino |
| author_sort | Marcela A S Pinhel |
| collection | DOAJ |
| description | Weight regulation and the magnitude of weight loss after a Roux-en-Y gastric bypass (RYGB) can be genetically determined. DNA methylation patterns and the expression of some genes can be altered after weight loss interventions, including RYGB. The present study aimed to evaluate how the gene expression and DNA methylation of <i>PIK3R1</i>, an obesity and insulin-related gene, change after RYGB. Blood samples were obtained from 13 women (35.9 ± 9.2 years) with severe obesity before and six months after surgical procedure. Whole blood transcriptome and epigenomic patterns were assessed by microarray-based, genome-wide technologies. A total of 1966 differentially expressed genes were identified in the pre- and postoperative periods of RYGB. From these, we observed that genes involved in obesity and insulin pathways were upregulated after surgery. Then, the <i>PIK3R1</i> gene was selected for further RT-qPCR analysis and cytosine-guanine nucleotide (CpG) sites methylation evaluation. We observed that the <i>PI3KR1</i> gene was upregulated, and six DNA methylation CpG sites were differently methylated after bariatric surgery. In conclusion, we found that RYGB upregulates genes involved in obesity and insulin pathways. |
| first_indexed | 2024-03-10T18:56:22Z |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T18:56:22Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-722c59393e4e48b1ab6d714fa27c66b22023-11-20T04:46:36ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-06-012112447610.3390/ijms21124476Changes in DNA Methylation and Gene Expression of Insulin and Obesity-Related Gene <i>PIK3R1</i> after Roux-en-Y Gastric BypassMarcela A S Pinhel0Natália Y Noronha1Carolina F Nicoletti2Vanessa AB Pereira3Bruno AP de Oliveira4Cristiana Cortes-Oliveira5Wilson Salgado6Fernando Barbosa7Júlio S Marchini8Doroteia RS Souza9Carla B Nonino10Laboratory of Nutrigenomics Studies, Health Science Department, Ribeirão Preto Medical School, Ribeirão Preto 14049-900, BrazilLaboratory of Nutrigenomics Studies, Health Science Department, Ribeirão Preto Medical School, Ribeirão Preto 14049-900, BrazilLaboratory of Nutrigenomics Studies, Health Science Department, Ribeirão Preto Medical School, Ribeirão Preto 14049-900, BrazilLaboratory of Nutrigenomics Studies, Health Science Department, Ribeirão Preto Medical School, Ribeirão Preto 14049-900, BrazilLaboratory of Nutrigenomics Studies, Health Science Department, Ribeirão Preto Medical School, Ribeirão Preto 14049-900, BrazilLaboratory of Nutrigenomics Studies, Health Science Department, Ribeirão Preto Medical School, Ribeirão Preto 14049-900, BrazilLaboratory of Nutrigenomics Studies, Health Science Department, Ribeirão Preto Medical School, Ribeirão Preto 14049-900, BrazilSchool of Pharmaceutical Sciences of Ribeirão Preto, Ribeirão Preto 14040-900, BrazilLaboratory of Nutrigenomics Studies, Health Science Department, Ribeirão Preto Medical School, Ribeirão Preto 14049-900, BrazilDepartment of Molecular Biology, São José do Rio Preto Medical School, São José do Rio Preto 15090-000, BrazilLaboratory of Nutrigenomics Studies, Health Science Department, Ribeirão Preto Medical School, Ribeirão Preto 14049-900, BrazilWeight regulation and the magnitude of weight loss after a Roux-en-Y gastric bypass (RYGB) can be genetically determined. DNA methylation patterns and the expression of some genes can be altered after weight loss interventions, including RYGB. The present study aimed to evaluate how the gene expression and DNA methylation of <i>PIK3R1</i>, an obesity and insulin-related gene, change after RYGB. Blood samples were obtained from 13 women (35.9 ± 9.2 years) with severe obesity before and six months after surgical procedure. Whole blood transcriptome and epigenomic patterns were assessed by microarray-based, genome-wide technologies. A total of 1966 differentially expressed genes were identified in the pre- and postoperative periods of RYGB. From these, we observed that genes involved in obesity and insulin pathways were upregulated after surgery. Then, the <i>PIK3R1</i> gene was selected for further RT-qPCR analysis and cytosine-guanine nucleotide (CpG) sites methylation evaluation. We observed that the <i>PI3KR1</i> gene was upregulated, and six DNA methylation CpG sites were differently methylated after bariatric surgery. In conclusion, we found that RYGB upregulates genes involved in obesity and insulin pathways.https://www.mdpi.com/1422-0067/21/12/4476DNA methylationinsulinobesitymicroarraygene expressionbariatric surgery |
| spellingShingle | Marcela A S Pinhel Natália Y Noronha Carolina F Nicoletti Vanessa AB Pereira Bruno AP de Oliveira Cristiana Cortes-Oliveira Wilson Salgado Fernando Barbosa Júlio S Marchini Doroteia RS Souza Carla B Nonino Changes in DNA Methylation and Gene Expression of Insulin and Obesity-Related Gene <i>PIK3R1</i> after Roux-en-Y Gastric Bypass International Journal of Molecular Sciences DNA methylation insulin obesity microarray gene expression bariatric surgery |
| title | Changes in DNA Methylation and Gene Expression of Insulin and Obesity-Related Gene <i>PIK3R1</i> after Roux-en-Y Gastric Bypass |
| title_full | Changes in DNA Methylation and Gene Expression of Insulin and Obesity-Related Gene <i>PIK3R1</i> after Roux-en-Y Gastric Bypass |
| title_fullStr | Changes in DNA Methylation and Gene Expression of Insulin and Obesity-Related Gene <i>PIK3R1</i> after Roux-en-Y Gastric Bypass |
| title_full_unstemmed | Changes in DNA Methylation and Gene Expression of Insulin and Obesity-Related Gene <i>PIK3R1</i> after Roux-en-Y Gastric Bypass |
| title_short | Changes in DNA Methylation and Gene Expression of Insulin and Obesity-Related Gene <i>PIK3R1</i> after Roux-en-Y Gastric Bypass |
| title_sort | changes in dna methylation and gene expression of insulin and obesity related gene i pik3r1 i after roux en y gastric bypass |
| topic | DNA methylation insulin obesity microarray gene expression bariatric surgery |
| url | https://www.mdpi.com/1422-0067/21/12/4476 |
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