Prognostic and Immune Infiltration Analysis of Transaldolase 1 (TALDO1) in Hepatocellular Carcinoma
Kedan Cen, Caide Lu Department of Hepatopancreatobiliary Surgery, The Affiliated Lihuili Hospital, Ningbo University, Ningbo, Zhejiang, People’s Republic of ChinaCorrespondence: Caide Lu, Department of Hepatopancreatobiliary Surgery, The Affiliated Lihuili Hospital, Ningbo University, 1111 Jiangnan...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2023-12-01
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| Series: | International Journal of General Medicine |
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| Online Access: | https://www.dovepress.com/prognostic-and-immune-infiltration-analysis-of-transaldolase-1-taldo1--peer-reviewed-fulltext-article-IJGM |
| _version_ | 1827590209503494144 |
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| author | Cen K Lu C |
| author_facet | Cen K Lu C |
| author_sort | Cen K |
| collection | DOAJ |
| description | Kedan Cen, Caide Lu Department of Hepatopancreatobiliary Surgery, The Affiliated Lihuili Hospital, Ningbo University, Ningbo, Zhejiang, People’s Republic of ChinaCorrespondence: Caide Lu, Department of Hepatopancreatobiliary Surgery, The Affiliated Lihuili Hospital, Ningbo University, 1111 Jiangnan Road, Ningbo, 315040, Zhejiang, People’s Republic of China, Email lucaide@nbu.edu.cnBackground: Transaldolase 1 (TALDO1) deficiency was associated with hepatocellular carcinoma (HCC), but the association between TALDO1 and prognosis in HCC remains unclear.Material and Methods: RNA-seq and clinical data of HCC from The Cancer Genome Atlas (TCGA) database and Gene Expression Omnibus (GEO) database were analyzed. CCK8 and EdU assays were utilized to examine the effect of TALDO1 on HCC proliferation. Transwell assay was used to explore the effect of TALDO1 on HCC migration. Western Blot was applied to detected the expression levels of pathway-related proteins.Results: TALDO1 mRNA level was higher in HCC tissues than in control normal tissues in TCGA and GEO databases (P< 0.001, P< 0.001). Expression of TALDO1 mRNA was associated with histologic grade (P=0.021). Multivariate regression analysis revealed that high TALDO1 mRNA expression was an independent risk factor for prognosis (P< 0.001). High expression of TALDO1 had poor overall survival (OS) (P=0.046). Additionally, Nomogram prognostic model of TALDO1 and clinicopathological parameters could predict the prognostic OS of HCC patients. Functional enrichment and immune infiltration analysis revealed that TALDO1 negatively regulates immune response. Furthermore, knockdown TALDO1 expression suppressed the proliferation and migration ability of HCC cells. Mechanistically, TALDO1 leaded to activation of the mitogen-activated protein kinase (MAPK) pathway and enhancement of epithelial-mesenchymal transition (EMT).Conclusion: Our findings demonstrated that TALDO1 could serve as a promising novel biomarker for HCC patients, which might modulate the immune microenvironment resulting in a poor prognosis.Keywords: transaldolase 1, prognosis, immune infiltration, proliferation, migration |
| first_indexed | 2024-03-09T01:13:41Z |
| format | Article |
| id | doaj.art-722d2c1d5f904242961f3500866663db |
| institution | Directory Open Access Journal |
| issn | 1178-7074 |
| language | English |
| last_indexed | 2024-03-09T01:13:41Z |
| publishDate | 2023-12-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of General Medicine |
| spelling | doaj.art-722d2c1d5f904242961f3500866663db2023-12-10T17:08:57ZengDove Medical PressInternational Journal of General Medicine1178-70742023-12-01Volume 165779578888877Prognostic and Immune Infiltration Analysis of Transaldolase 1 (TALDO1) in Hepatocellular CarcinomaCen KLu CKedan Cen, Caide Lu Department of Hepatopancreatobiliary Surgery, The Affiliated Lihuili Hospital, Ningbo University, Ningbo, Zhejiang, People’s Republic of ChinaCorrespondence: Caide Lu, Department of Hepatopancreatobiliary Surgery, The Affiliated Lihuili Hospital, Ningbo University, 1111 Jiangnan Road, Ningbo, 315040, Zhejiang, People’s Republic of China, Email lucaide@nbu.edu.cnBackground: Transaldolase 1 (TALDO1) deficiency was associated with hepatocellular carcinoma (HCC), but the association between TALDO1 and prognosis in HCC remains unclear.Material and Methods: RNA-seq and clinical data of HCC from The Cancer Genome Atlas (TCGA) database and Gene Expression Omnibus (GEO) database were analyzed. CCK8 and EdU assays were utilized to examine the effect of TALDO1 on HCC proliferation. Transwell assay was used to explore the effect of TALDO1 on HCC migration. Western Blot was applied to detected the expression levels of pathway-related proteins.Results: TALDO1 mRNA level was higher in HCC tissues than in control normal tissues in TCGA and GEO databases (P< 0.001, P< 0.001). Expression of TALDO1 mRNA was associated with histologic grade (P=0.021). Multivariate regression analysis revealed that high TALDO1 mRNA expression was an independent risk factor for prognosis (P< 0.001). High expression of TALDO1 had poor overall survival (OS) (P=0.046). Additionally, Nomogram prognostic model of TALDO1 and clinicopathological parameters could predict the prognostic OS of HCC patients. Functional enrichment and immune infiltration analysis revealed that TALDO1 negatively regulates immune response. Furthermore, knockdown TALDO1 expression suppressed the proliferation and migration ability of HCC cells. Mechanistically, TALDO1 leaded to activation of the mitogen-activated protein kinase (MAPK) pathway and enhancement of epithelial-mesenchymal transition (EMT).Conclusion: Our findings demonstrated that TALDO1 could serve as a promising novel biomarker for HCC patients, which might modulate the immune microenvironment resulting in a poor prognosis.Keywords: transaldolase 1, prognosis, immune infiltration, proliferation, migrationhttps://www.dovepress.com/prognostic-and-immune-infiltration-analysis-of-transaldolase-1-taldo1--peer-reviewed-fulltext-article-IJGMtransaldolase 1prognosisimmune infiltrationproliferationmigration |
| spellingShingle | Cen K Lu C Prognostic and Immune Infiltration Analysis of Transaldolase 1 (TALDO1) in Hepatocellular Carcinoma International Journal of General Medicine transaldolase 1 prognosis immune infiltration proliferation migration |
| title | Prognostic and Immune Infiltration Analysis of Transaldolase 1 (TALDO1) in Hepatocellular Carcinoma |
| title_full | Prognostic and Immune Infiltration Analysis of Transaldolase 1 (TALDO1) in Hepatocellular Carcinoma |
| title_fullStr | Prognostic and Immune Infiltration Analysis of Transaldolase 1 (TALDO1) in Hepatocellular Carcinoma |
| title_full_unstemmed | Prognostic and Immune Infiltration Analysis of Transaldolase 1 (TALDO1) in Hepatocellular Carcinoma |
| title_short | Prognostic and Immune Infiltration Analysis of Transaldolase 1 (TALDO1) in Hepatocellular Carcinoma |
| title_sort | prognostic and immune infiltration analysis of transaldolase 1 taldo1 in hepatocellular carcinoma |
| topic | transaldolase 1 prognosis immune infiltration proliferation migration |
| url | https://www.dovepress.com/prognostic-and-immune-infiltration-analysis-of-transaldolase-1-taldo1--peer-reviewed-fulltext-article-IJGM |
| work_keys_str_mv | AT cenk prognosticandimmuneinfiltrationanalysisoftransaldolase1taldo1inhepatocellularcarcinoma AT luc prognosticandimmuneinfiltrationanalysisoftransaldolase1taldo1inhepatocellularcarcinoma |