The complete genome sequence of the archaeal isolate Halomicrobium sp. ZPS1 reveals the nitrogen metabolism characteristics under hypersaline conditions
Abstract Purpose As a potential tool for the biodegradation of nitrogen contaminants, including nitrate, nitrite, and ammonium, in pickled foods with high salinity, the halophilic and denitrifying archaeal strain Halomicrobium sp. ZPS1 was isolated from edible salt particles. Methods Under anaerobic...
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| Format: | Article |
| Language: | English |
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BMC
2020-05-01
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| Series: | Annals of Microbiology |
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| Online Access: | https://doi.org/10.1186/s13213-020-01575-8 |
| _version_ | 1819088968624373760 |
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| author | Xinyu Hu Chunlin Zeng Zhaozhi Hou Yuan Wang Qin Xu Kazuo Isobe Keishi Senoo Lin Zhu |
| author_facet | Xinyu Hu Chunlin Zeng Zhaozhi Hou Yuan Wang Qin Xu Kazuo Isobe Keishi Senoo Lin Zhu |
| author_sort | Xinyu Hu |
| collection | DOAJ |
| description | Abstract Purpose As a potential tool for the biodegradation of nitrogen contaminants, including nitrate, nitrite, and ammonium, in pickled foods with high salinity, the halophilic and denitrifying archaeal strain Halomicrobium sp. ZPS1 was isolated from edible salt particles. Methods Under anaerobic and static culture conditions, Halomicrobium sp. ZPS1 could simultaneously degrade nitrate, nitrite, and ammonium in liquid medium with 18% salinity and generate N2O. To gain insight into these physiological characteristics, the complete genome of Halomicrobium sp. ZPS1 was sequenced to reveal the mechanism of nitrogen metabolism associated with salt-tolerance. Result The complete genome sequencing revealed a genome size of 3,094,203 bp with a circular chromosome and a GC content of 65.64%. Based on gene annotation, 3191 CDSs, 6 rRNA genes, and 76 tRNA genes were identified. Moreover, 28 genes were annotated as related to salt tolerance, ammonium assimilation, and a truncated denitrification pathway. Conclusion The annotated functional genes indicate that Halomicrobium sp. ZPS1 could be a candidate strain for the simultaneous removal of nitrate, nitrite, and ammonia in extremely high salt environments. |
| first_indexed | 2024-12-21T22:00:28Z |
| format | Article |
| id | doaj.art-722da55162db4deab098c2a328f647d8 |
| institution | Directory Open Access Journal |
| issn | 1590-4261 1869-2044 |
| language | English |
| last_indexed | 2024-12-21T22:00:28Z |
| publishDate | 2020-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Annals of Microbiology |
| spelling | doaj.art-722da55162db4deab098c2a328f647d82022-12-21T18:48:50ZengBMCAnnals of Microbiology1590-42611869-20442020-05-017011910.1186/s13213-020-01575-8The complete genome sequence of the archaeal isolate Halomicrobium sp. ZPS1 reveals the nitrogen metabolism characteristics under hypersaline conditionsXinyu Hu0Chunlin Zeng1Zhaozhi Hou2Yuan Wang3Qin Xu4Kazuo Isobe5Keishi Senoo6Lin Zhu7School of Agricultural Equipment Engineering, Jiangsu UniversitySchool of Food and Biological Engineering, Jiangsu UniversitySchool of Food and Biological Engineering, Jiangsu UniversitySchool of Food and Biological Engineering, Jiangsu UniversitySchool of Food and Biological Engineering, Jiangsu UniversityDepartment of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of TokyoDepartment of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of TokyoSchool of Food and Biological Engineering, Jiangsu UniversityAbstract Purpose As a potential tool for the biodegradation of nitrogen contaminants, including nitrate, nitrite, and ammonium, in pickled foods with high salinity, the halophilic and denitrifying archaeal strain Halomicrobium sp. ZPS1 was isolated from edible salt particles. Methods Under anaerobic and static culture conditions, Halomicrobium sp. ZPS1 could simultaneously degrade nitrate, nitrite, and ammonium in liquid medium with 18% salinity and generate N2O. To gain insight into these physiological characteristics, the complete genome of Halomicrobium sp. ZPS1 was sequenced to reveal the mechanism of nitrogen metabolism associated with salt-tolerance. Result The complete genome sequencing revealed a genome size of 3,094,203 bp with a circular chromosome and a GC content of 65.64%. Based on gene annotation, 3191 CDSs, 6 rRNA genes, and 76 tRNA genes were identified. Moreover, 28 genes were annotated as related to salt tolerance, ammonium assimilation, and a truncated denitrification pathway. Conclusion The annotated functional genes indicate that Halomicrobium sp. ZPS1 could be a candidate strain for the simultaneous removal of nitrate, nitrite, and ammonia in extremely high salt environments.https://doi.org/10.1186/s13213-020-01575-8Halomicrobium sp.Salt toleranceAmmonia assimilationDenitrification |
| spellingShingle | Xinyu Hu Chunlin Zeng Zhaozhi Hou Yuan Wang Qin Xu Kazuo Isobe Keishi Senoo Lin Zhu The complete genome sequence of the archaeal isolate Halomicrobium sp. ZPS1 reveals the nitrogen metabolism characteristics under hypersaline conditions Annals of Microbiology Halomicrobium sp. Salt tolerance Ammonia assimilation Denitrification |
| title | The complete genome sequence of the archaeal isolate Halomicrobium sp. ZPS1 reveals the nitrogen metabolism characteristics under hypersaline conditions |
| title_full | The complete genome sequence of the archaeal isolate Halomicrobium sp. ZPS1 reveals the nitrogen metabolism characteristics under hypersaline conditions |
| title_fullStr | The complete genome sequence of the archaeal isolate Halomicrobium sp. ZPS1 reveals the nitrogen metabolism characteristics under hypersaline conditions |
| title_full_unstemmed | The complete genome sequence of the archaeal isolate Halomicrobium sp. ZPS1 reveals the nitrogen metabolism characteristics under hypersaline conditions |
| title_short | The complete genome sequence of the archaeal isolate Halomicrobium sp. ZPS1 reveals the nitrogen metabolism characteristics under hypersaline conditions |
| title_sort | complete genome sequence of the archaeal isolate halomicrobium sp zps1 reveals the nitrogen metabolism characteristics under hypersaline conditions |
| topic | Halomicrobium sp. Salt tolerance Ammonia assimilation Denitrification |
| url | https://doi.org/10.1186/s13213-020-01575-8 |
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