Identification of MicroRNAs Regulating <i>Clostridium perfringens</i> Type C Infection in the Spleen of Diarrheic Piglets
<i>Clostridium perfringens</i> (<i>C. perfringens</i>) type C is one of the common bacteria in piglet diarrhea, which seriously affects the swine industry’s development. The spleen plays crucial roles in the resistance and elimination of pathogenic microorganisms, and miRNAs...
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MDPI AG
2023-04-01
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| Series: | Current Issues in Molecular Biology |
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| Online Access: | https://www.mdpi.com/1467-3045/45/4/208 |
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| author | Pengfei Wang Qiaoli Yang Zunqiang Yan Xiaoyu Huang Xiaoli Gao Shuangbao Gun |
| author_facet | Pengfei Wang Qiaoli Yang Zunqiang Yan Xiaoyu Huang Xiaoli Gao Shuangbao Gun |
| author_sort | Pengfei Wang |
| collection | DOAJ |
| description | <i>Clostridium perfringens</i> (<i>C. perfringens</i>) type C is one of the common bacteria in piglet diarrhea, which seriously affects the swine industry’s development. The spleen plays crucial roles in the resistance and elimination of pathogenic microorganisms, and miRNAs play important roles in regulating piglet diarrhea caused by pathogens. However, the mechanism by which miRNAs in the spleen are involved in regulating <i>C. perfringens</i> type C causing diarrhea in piglets remains unclear. The expression profiles of the spleen miRNAs of 7-day-old piglets challenged by <i>C. perfringens</i> type C were studied using small RNA-sequencing in control (SC), susceptible (SS), and resistant (SR) groups. Eight-eight differentially expressed miRNAs were screened. The KEGG pathway analysis of target genes revealed that the miRNAs were involved in the MAPK, p53, and ECM–receptor interaction signaling pathways. <i>NFATC4</i> was determined to be a direct target of miR-532-3p and miR-133b using a dual-luciferase reporter assay. Thus, miR-133b and miR-532-3p targeted to <i>NFATC4</i> were likely involved to piglet resistance to <i>C. perfringens</i> type C. This paper provides the valuable resources to deeply understand the genetic basis of <i>C. perfringens</i> type C resistance in piglets and a solid foundation to identify novel markers of <i>C. perfringens</i> type C resistance. |
| first_indexed | 2024-03-11T05:08:04Z |
| format | Article |
| id | doaj.art-722fb57fe2ed49debf3660531925199d |
| institution | Directory Open Access Journal |
| issn | 1467-3037 1467-3045 |
| language | English |
| last_indexed | 2024-03-11T05:08:04Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Current Issues in Molecular Biology |
| spelling | doaj.art-722fb57fe2ed49debf3660531925199d2023-11-17T18:47:48ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452023-04-014543193320710.3390/cimb45040208Identification of MicroRNAs Regulating <i>Clostridium perfringens</i> Type C Infection in the Spleen of Diarrheic PigletsPengfei Wang0Qiaoli Yang1Zunqiang Yan2Xiaoyu Huang3Xiaoli Gao4Shuangbao Gun5College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China<i>Clostridium perfringens</i> (<i>C. perfringens</i>) type C is one of the common bacteria in piglet diarrhea, which seriously affects the swine industry’s development. The spleen plays crucial roles in the resistance and elimination of pathogenic microorganisms, and miRNAs play important roles in regulating piglet diarrhea caused by pathogens. However, the mechanism by which miRNAs in the spleen are involved in regulating <i>C. perfringens</i> type C causing diarrhea in piglets remains unclear. The expression profiles of the spleen miRNAs of 7-day-old piglets challenged by <i>C. perfringens</i> type C were studied using small RNA-sequencing in control (SC), susceptible (SS), and resistant (SR) groups. Eight-eight differentially expressed miRNAs were screened. The KEGG pathway analysis of target genes revealed that the miRNAs were involved in the MAPK, p53, and ECM–receptor interaction signaling pathways. <i>NFATC4</i> was determined to be a direct target of miR-532-3p and miR-133b using a dual-luciferase reporter assay. Thus, miR-133b and miR-532-3p targeted to <i>NFATC4</i> were likely involved to piglet resistance to <i>C. perfringens</i> type C. This paper provides the valuable resources to deeply understand the genetic basis of <i>C. perfringens</i> type C resistance in piglets and a solid foundation to identify novel markers of <i>C. perfringens</i> type C resistance.https://www.mdpi.com/1467-3045/45/4/208pigletdiarrheaspleenmiRNA<i>C. perfringens</i> type Cresistance |
| spellingShingle | Pengfei Wang Qiaoli Yang Zunqiang Yan Xiaoyu Huang Xiaoli Gao Shuangbao Gun Identification of MicroRNAs Regulating <i>Clostridium perfringens</i> Type C Infection in the Spleen of Diarrheic Piglets Current Issues in Molecular Biology piglet diarrhea spleen miRNA <i>C. perfringens</i> type C resistance |
| title | Identification of MicroRNAs Regulating <i>Clostridium perfringens</i> Type C Infection in the Spleen of Diarrheic Piglets |
| title_full | Identification of MicroRNAs Regulating <i>Clostridium perfringens</i> Type C Infection in the Spleen of Diarrheic Piglets |
| title_fullStr | Identification of MicroRNAs Regulating <i>Clostridium perfringens</i> Type C Infection in the Spleen of Diarrheic Piglets |
| title_full_unstemmed | Identification of MicroRNAs Regulating <i>Clostridium perfringens</i> Type C Infection in the Spleen of Diarrheic Piglets |
| title_short | Identification of MicroRNAs Regulating <i>Clostridium perfringens</i> Type C Infection in the Spleen of Diarrheic Piglets |
| title_sort | identification of micrornas regulating i clostridium perfringens i type c infection in the spleen of diarrheic piglets |
| topic | piglet diarrhea spleen miRNA <i>C. perfringens</i> type C resistance |
| url | https://www.mdpi.com/1467-3045/45/4/208 |
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