Lab-on-Chip Cytometry Based on Magnetoresistive Sensors for Bacteria Detection in Milk
Flow cytometers have been optimized for use in portable platforms, where cell separation, identification and counting can be achieved in a compact and modular format. This feature can be combined with magnetic detection, where magnetoresistive sensors can be integrated within microfluidic channels t...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2014-08-01
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| Series: | Sensors |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1424-8220/14/8/15496 |
| _version_ | 1811184908627869696 |
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| author | Ana C. Fernandes Carla M. Duarte Filipe A. Cardoso Ricardo Bexiga Susana Cardoso Paulo P. Freitas |
| author_facet | Ana C. Fernandes Carla M. Duarte Filipe A. Cardoso Ricardo Bexiga Susana Cardoso Paulo P. Freitas |
| author_sort | Ana C. Fernandes |
| collection | DOAJ |
| description | Flow cytometers have been optimized for use in portable platforms, where cell separation, identification and counting can be achieved in a compact and modular format. This feature can be combined with magnetic detection, where magnetoresistive sensors can be integrated within microfluidic channels to detect magnetically labelled cells. This work describes a platform for in-flow detection of magnetically labelled cells with a magneto-resistive based cell cytometer. In particular, we present an example for the validation of the platform as a magnetic counter that identifies and quantifies Streptococcus agalactiae in milk. |
| first_indexed | 2024-04-11T13:20:10Z |
| format | Article |
| id | doaj.art-723071beb64340eea7caaf5c37938ee1 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T13:20:10Z |
| publishDate | 2014-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-723071beb64340eea7caaf5c37938ee12022-12-22T04:22:14ZengMDPI AGSensors1424-82202014-08-01148154961552410.3390/s140815496s140815496Lab-on-Chip Cytometry Based on Magnetoresistive Sensors for Bacteria Detection in MilkAna C. Fernandes0Carla M. Duarte1Filipe A. Cardoso2Ricardo Bexiga3Susana Cardoso4Paulo P. Freitas5INESC–MN—Instituto de Engenharia de Sistemas e Computadores–Microsistemas e Nanotecnologias and IN—Institute of Nanoscience and Nanotechnology, Rua Alves Redol 9, 1000-029 Lisbon, PortugalINESC–MN—Instituto de Engenharia de Sistemas e Computadores–Microsistemas e Nanotecnologias and IN—Institute of Nanoscience and Nanotechnology, Rua Alves Redol 9, 1000-029 Lisbon, PortugalINESC–MN—Instituto de Engenharia de Sistemas e Computadores–Microsistemas e Nanotecnologias and IN—Institute of Nanoscience and Nanotechnology, Rua Alves Redol 9, 1000-029 Lisbon, PortugalCIISA, Veterinary Medicine Faculty, Technical University of Lisbon, Av. da Universidade Técnica, 1300-477 Lisbon, PortugalINESC–MN—Instituto de Engenharia de Sistemas e Computadores–Microsistemas e Nanotecnologias and IN—Institute of Nanoscience and Nanotechnology, Rua Alves Redol 9, 1000-029 Lisbon, PortugalINESC–MN—Instituto de Engenharia de Sistemas e Computadores–Microsistemas e Nanotecnologias and IN—Institute of Nanoscience and Nanotechnology, Rua Alves Redol 9, 1000-029 Lisbon, PortugalFlow cytometers have been optimized for use in portable platforms, where cell separation, identification and counting can be achieved in a compact and modular format. This feature can be combined with magnetic detection, where magnetoresistive sensors can be integrated within microfluidic channels to detect magnetically labelled cells. This work describes a platform for in-flow detection of magnetically labelled cells with a magneto-resistive based cell cytometer. In particular, we present an example for the validation of the platform as a magnetic counter that identifies and quantifies Streptococcus agalactiae in milk.http://www.mdpi.com/1424-8220/14/8/15496cytometermicrofluidicmagnetoresistive sensormilkStreptococcus agalactiae |
| spellingShingle | Ana C. Fernandes Carla M. Duarte Filipe A. Cardoso Ricardo Bexiga Susana Cardoso Paulo P. Freitas Lab-on-Chip Cytometry Based on Magnetoresistive Sensors for Bacteria Detection in Milk Sensors cytometer microfluidic magnetoresistive sensor milk Streptococcus agalactiae |
| title | Lab-on-Chip Cytometry Based on Magnetoresistive Sensors for Bacteria Detection in Milk |
| title_full | Lab-on-Chip Cytometry Based on Magnetoresistive Sensors for Bacteria Detection in Milk |
| title_fullStr | Lab-on-Chip Cytometry Based on Magnetoresistive Sensors for Bacteria Detection in Milk |
| title_full_unstemmed | Lab-on-Chip Cytometry Based on Magnetoresistive Sensors for Bacteria Detection in Milk |
| title_short | Lab-on-Chip Cytometry Based on Magnetoresistive Sensors for Bacteria Detection in Milk |
| title_sort | lab on chip cytometry based on magnetoresistive sensors for bacteria detection in milk |
| topic | cytometer microfluidic magnetoresistive sensor milk Streptococcus agalactiae |
| url | http://www.mdpi.com/1424-8220/14/8/15496 |
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