Systemic mapping of organ plasma extravasation at multiple stages of chronic heart failure
Introduction: Chronic Heart failure (CHF) is a highly prevalent disease that leads to significant morbidity and mortality. Diffuse vasculopathy is a commonmorbidity associated with CHF. Increased vascular permeability leading to plasma extravasation (PEx) occurs in surrounding tissues following endo...
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| Format: | Article |
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Frontiers Media S.A.
2023-11-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2023.1288907/full |
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| author | Oliver Kitzerow Oliver Kitzerow Paul Suder Mohanad Shukry Steven J. Lisco Irving H. Zucker Han-Jun Wang |
| author_facet | Oliver Kitzerow Oliver Kitzerow Paul Suder Mohanad Shukry Steven J. Lisco Irving H. Zucker Han-Jun Wang |
| author_sort | Oliver Kitzerow |
| collection | DOAJ |
| description | Introduction: Chronic Heart failure (CHF) is a highly prevalent disease that leads to significant morbidity and mortality. Diffuse vasculopathy is a commonmorbidity associated with CHF. Increased vascular permeability leading to plasma extravasation (PEx) occurs in surrounding tissues following endothelial dysfunction. Such micro- and macrovascular complications develop over time and lead to edema, inflammation, and multi-organ dysfunction in CHF. However, a systemic examination of PEx in vital organs among different time windows of CHF has never been performed. In the present study, we investigated time-dependent PEx in several major visceral organs including heart, lung, liver, spleen, kidney, duodenum, ileum, cecum, and pancreas between sham-operated and CHF rats induced by myocardial infarction (MI).Methods: Plasma extravasation was determined by colorimetric evaluation of Evans Blue (EB) concentrations at 3 days, ∼10 weeks and 4 months following MI.Results: Data show that cardiac PEx was initially high at day 3 post MI and then gradually decreased but remained at a moderately high level at ∼10 weeks and 4 months post MI. Lung PEx began at day 3 and remained significantly elevated at both ∼10 weeks and 4 months post MI. Spleen PExwas significantly increased at ∼10 weeks and 4 months but not on day 3 post MI. Liver PEx occurred early at day 3 and remain significantly increased at ∼10 weeks and 4 months post MI. For the gastrointestinal (GI) organs including duodenum, ileum and cecum, there was a general trend that PEx level gradually increased following MI and reached statistical significance at either 10 weeks or 4 months post MI. Similar to GI PEx, renal PEx was significantly elevated at 4 months post MI.Discussion: In summary, we found that MI generally incites a timedependent PEx of multiple visceral organs. However, the PEx time window for individual organs in response to the MI challenge was different, suggesting that different mechanisms are involved in the pathogenesis of PEx in these vital organs during the development of CHF. |
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| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-03-11T10:13:50Z |
| publishDate | 2023-11-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Physiology |
| spelling | doaj.art-7232d50d89184167b8343b41609dd7742023-11-16T12:56:50ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2023-11-011410.3389/fphys.2023.12889071288907Systemic mapping of organ plasma extravasation at multiple stages of chronic heart failureOliver Kitzerow0Oliver Kitzerow1Paul Suder2Mohanad Shukry3Steven J. Lisco4Irving H. Zucker5Han-Jun Wang6Department of Genetics Cell Biology and Anatomy, University of Nebraska Medical Center, Omaha, NE, United StatesDepartment of Anesthesiology, University of Nebraska Medical Center, Omaha, NE, United StatesDepartment of Anesthesiology, University of Nebraska Medical Center, Omaha, NE, United StatesDepartment of Anesthesiology, University of Nebraska Medical Center, Omaha, NE, United StatesDepartment of Anesthesiology, University of Nebraska Medical Center, Omaha, NE, United StatesDepartment of Cellular and Integrative Physiology, University of Nebraska Medical Center, Omaha, NE, United StatesDepartment of Anesthesiology, University of Nebraska Medical Center, Omaha, NE, United StatesIntroduction: Chronic Heart failure (CHF) is a highly prevalent disease that leads to significant morbidity and mortality. Diffuse vasculopathy is a commonmorbidity associated with CHF. Increased vascular permeability leading to plasma extravasation (PEx) occurs in surrounding tissues following endothelial dysfunction. Such micro- and macrovascular complications develop over time and lead to edema, inflammation, and multi-organ dysfunction in CHF. However, a systemic examination of PEx in vital organs among different time windows of CHF has never been performed. In the present study, we investigated time-dependent PEx in several major visceral organs including heart, lung, liver, spleen, kidney, duodenum, ileum, cecum, and pancreas between sham-operated and CHF rats induced by myocardial infarction (MI).Methods: Plasma extravasation was determined by colorimetric evaluation of Evans Blue (EB) concentrations at 3 days, ∼10 weeks and 4 months following MI.Results: Data show that cardiac PEx was initially high at day 3 post MI and then gradually decreased but remained at a moderately high level at ∼10 weeks and 4 months post MI. Lung PEx began at day 3 and remained significantly elevated at both ∼10 weeks and 4 months post MI. Spleen PExwas significantly increased at ∼10 weeks and 4 months but not on day 3 post MI. Liver PEx occurred early at day 3 and remain significantly increased at ∼10 weeks and 4 months post MI. For the gastrointestinal (GI) organs including duodenum, ileum and cecum, there was a general trend that PEx level gradually increased following MI and reached statistical significance at either 10 weeks or 4 months post MI. Similar to GI PEx, renal PEx was significantly elevated at 4 months post MI.Discussion: In summary, we found that MI generally incites a timedependent PEx of multiple visceral organs. However, the PEx time window for individual organs in response to the MI challenge was different, suggesting that different mechanisms are involved in the pathogenesis of PEx in these vital organs during the development of CHF.https://www.frontiersin.org/articles/10.3389/fphys.2023.1288907/fullinflammationchronic heart failurepancreasgastro-intestinal dysfunctionpulmonary hypertension |
| spellingShingle | Oliver Kitzerow Oliver Kitzerow Paul Suder Mohanad Shukry Steven J. Lisco Irving H. Zucker Han-Jun Wang Systemic mapping of organ plasma extravasation at multiple stages of chronic heart failure Frontiers in Physiology inflammation chronic heart failure pancreas gastro-intestinal dysfunction pulmonary hypertension |
| title | Systemic mapping of organ plasma extravasation at multiple stages of chronic heart failure |
| title_full | Systemic mapping of organ plasma extravasation at multiple stages of chronic heart failure |
| title_fullStr | Systemic mapping of organ plasma extravasation at multiple stages of chronic heart failure |
| title_full_unstemmed | Systemic mapping of organ plasma extravasation at multiple stages of chronic heart failure |
| title_short | Systemic mapping of organ plasma extravasation at multiple stages of chronic heart failure |
| title_sort | systemic mapping of organ plasma extravasation at multiple stages of chronic heart failure |
| topic | inflammation chronic heart failure pancreas gastro-intestinal dysfunction pulmonary hypertension |
| url | https://www.frontiersin.org/articles/10.3389/fphys.2023.1288907/full |
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