Cell-derived artificial nanovesicle as a drug delivery system for malignant melanoma treatment
Extracellular vehicles have a natural targeting ability and immune tolerance of being usually applied in drug delivery systems; however, the purification of EVs is complicated and the production yield was quite low. We developed an artificial cellular mimetic nanovesicle (NV) with melanoma fragment...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-03-01
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| Series: | Biomedicine & Pharmacotherapy |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0753332221013731 |
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| author | Ying-Yi Lin Chung-Yi Chen Dik-Lung Ma Chung-Hang Leung Chu-Yu Chang Hui-Min David Wang |
| author_facet | Ying-Yi Lin Chung-Yi Chen Dik-Lung Ma Chung-Hang Leung Chu-Yu Chang Hui-Min David Wang |
| author_sort | Ying-Yi Lin |
| collection | DOAJ |
| description | Extracellular vehicles have a natural targeting ability and immune tolerance of being usually applied in drug delivery systems; however, the purification of EVs is complicated and the production yield was quite low. We developed an artificial cellular mimetic nanovesicle (NV) with melanoma fragment membrane for the transportation with curcumin to achieve the anticancer purpose. B16F10 derived NVs were manufactured by the breakdown of cells using a series of extrusions through cut-off size filters (10 and 5 µm), and the whole procedure was easy and time-saving. To terminate the suspicion of cancer metastatic issue, B16F10 cells were treated by 30-min sonication and 1-min UVB exposure to remove genetic materials before the extrusion. B16F10 derived NV loaded with curcumin was called NV(S30U1/Cur), and the anticancer effect was evaluated by cell-based viability, immune, migration, and invasion. The results showed that NVs were manufactured by passing through 10 and 5 µm filters having an enviable production yield, and the mRNA amounts were declined within NVs produced by B16F10 cells treated with UVB in a comparison to the control group. NV(S30U1/Cur) were effectively decreased B1610 cell viability, and migratory and invasive abilities were also reduced significantly. Besides, CD8+ expression of murine primary lymphocytes was activated with CD4+ reduction by NV(S30U1/Cur) to stimulate the inherent tumor suppressive capacity in the immune system. Taken together, we established bioengineered NVs serving as novel cell mimetic nanocarriers to deliver natural compound for malignant melanoma potential immune chemotherapy. Data Availability Statement: The data used to support the findings of this study are available from the corresponding author upon requests. |
| first_indexed | 2024-04-11T21:01:06Z |
| format | Article |
| id | doaj.art-7234a9b53145460f8be6e0eea735ab43 |
| institution | Directory Open Access Journal |
| issn | 0753-3322 |
| language | English |
| last_indexed | 2024-04-11T21:01:06Z |
| publishDate | 2022-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biomedicine & Pharmacotherapy |
| spelling | doaj.art-7234a9b53145460f8be6e0eea735ab432022-12-22T04:03:30ZengElsevierBiomedicine & Pharmacotherapy0753-33222022-03-01147112586Cell-derived artificial nanovesicle as a drug delivery system for malignant melanoma treatmentYing-Yi Lin0Chung-Yi Chen1Dik-Lung Ma2Chung-Hang Leung3Chu-Yu Chang4Hui-Min David Wang5Graduate Institute of Biomedical Engineering, National Chung Hsing University, Taichung City 402, TaiwanDepartment of Nutrition and Health Science, School of Medical and Health Sciences, Fooyin University, Kaohsiung City 83102, TaiwanDepartment of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong, ChinaInstitute of Chinese Medical Sciences, University of Macau, Taipa, Macau, ChinaGraduate Institute of Biomedical Engineering, National Chung Hsing University, Taichung City 402, TaiwanGraduate Institute of Biomedical Engineering, National Chung Hsing University, Taichung City 402, Taiwan; Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung City, Taiwan; Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung City, Taiwan; Correspondence to: Graduate Institute of Biomedical Engineering, National Chung Hsing University, Taichung 402, Taiwan.Extracellular vehicles have a natural targeting ability and immune tolerance of being usually applied in drug delivery systems; however, the purification of EVs is complicated and the production yield was quite low. We developed an artificial cellular mimetic nanovesicle (NV) with melanoma fragment membrane for the transportation with curcumin to achieve the anticancer purpose. B16F10 derived NVs were manufactured by the breakdown of cells using a series of extrusions through cut-off size filters (10 and 5 µm), and the whole procedure was easy and time-saving. To terminate the suspicion of cancer metastatic issue, B16F10 cells were treated by 30-min sonication and 1-min UVB exposure to remove genetic materials before the extrusion. B16F10 derived NV loaded with curcumin was called NV(S30U1/Cur), and the anticancer effect was evaluated by cell-based viability, immune, migration, and invasion. The results showed that NVs were manufactured by passing through 10 and 5 µm filters having an enviable production yield, and the mRNA amounts were declined within NVs produced by B16F10 cells treated with UVB in a comparison to the control group. NV(S30U1/Cur) were effectively decreased B1610 cell viability, and migratory and invasive abilities were also reduced significantly. Besides, CD8+ expression of murine primary lymphocytes was activated with CD4+ reduction by NV(S30U1/Cur) to stimulate the inherent tumor suppressive capacity in the immune system. Taken together, we established bioengineered NVs serving as novel cell mimetic nanocarriers to deliver natural compound for malignant melanoma potential immune chemotherapy. Data Availability Statement: The data used to support the findings of this study are available from the corresponding author upon requests.http://www.sciencedirect.com/science/article/pii/S0753332221013731NanovesicleCurcuminMelanomaImmuneMetastasis |
| spellingShingle | Ying-Yi Lin Chung-Yi Chen Dik-Lung Ma Chung-Hang Leung Chu-Yu Chang Hui-Min David Wang Cell-derived artificial nanovesicle as a drug delivery system for malignant melanoma treatment Biomedicine & Pharmacotherapy Nanovesicle Curcumin Melanoma Immune Metastasis |
| title | Cell-derived artificial nanovesicle as a drug delivery system for malignant melanoma treatment |
| title_full | Cell-derived artificial nanovesicle as a drug delivery system for malignant melanoma treatment |
| title_fullStr | Cell-derived artificial nanovesicle as a drug delivery system for malignant melanoma treatment |
| title_full_unstemmed | Cell-derived artificial nanovesicle as a drug delivery system for malignant melanoma treatment |
| title_short | Cell-derived artificial nanovesicle as a drug delivery system for malignant melanoma treatment |
| title_sort | cell derived artificial nanovesicle as a drug delivery system for malignant melanoma treatment |
| topic | Nanovesicle Curcumin Melanoma Immune Metastasis |
| url | http://www.sciencedirect.com/science/article/pii/S0753332221013731 |
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