Lactate is useful for the efficient replication of porcine epidemic diarrhea virus in cell culture
Porcine epidemic diarrhea virus (PEDV) is a deadly pathogen infecting pig herds, and has caused significant economic losses around the world. Vaccination remains the most effective way of keeping the PEDV epidemic under control. Previous studies have shown that the host metabolism has a significant...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-02-01
|
| Series: | Frontiers in Veterinary Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2023.1116695/full |
| _version_ | 1828034728857436160 |
|---|---|
| author | Nile Wuri Nile Wuri Hongchao Gou Hongchao Gou Bin Zhang Bin Zhang Menglu Wang Menglu Wang Songqi Wang Songqi Wang Weixiao Zhang Weixiao Zhang Haiyan He Haiyan He Xuelei Fan Xuelei Fan Chunhong Zhang Chunhong Zhang Zhicheng Liu Zhicheng Liu Letu Geri Haiyan Shen Haiyan Shen Jianfeng Zhang Jianfeng Zhang |
| author_facet | Nile Wuri Nile Wuri Hongchao Gou Hongchao Gou Bin Zhang Bin Zhang Menglu Wang Menglu Wang Songqi Wang Songqi Wang Weixiao Zhang Weixiao Zhang Haiyan He Haiyan He Xuelei Fan Xuelei Fan Chunhong Zhang Chunhong Zhang Zhicheng Liu Zhicheng Liu Letu Geri Haiyan Shen Haiyan Shen Jianfeng Zhang Jianfeng Zhang |
| author_sort | Nile Wuri |
| collection | DOAJ |
| description | Porcine epidemic diarrhea virus (PEDV) is a deadly pathogen infecting pig herds, and has caused significant economic losses around the world. Vaccination remains the most effective way of keeping the PEDV epidemic under control. Previous studies have shown that the host metabolism has a significant impact on viral replication. In this study, we have demonstrated that two substrates of metabolic pathway, glucose and glutamine, play a key role in PEDV replication. Interestingly, the boosting effect of these compounds toward viral replication appeared to be dose-independent. Furthermore, we found that lactate, which is a downstream metabolite, promotes PEDV replication, even when added in excess to the cell culture medium. Moreover, the role of lactate in promoting PEDV was independent of the genotype of PEDV and the multiplicity of infection (MOI). Our findings suggest that lactate is a promising candidate for use as a cell culture additive for promoting PEDV replication. It could improve the efficiency of vaccine production and provide the basis for designing novel antiviral strategies. |
| first_indexed | 2024-04-10T15:36:41Z |
| format | Article |
| id | doaj.art-7235dcb7b8dc4113a99393b06fac7f4a |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-04-10T15:36:41Z |
| publishDate | 2023-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-7235dcb7b8dc4113a99393b06fac7f4a2023-02-13T04:49:14ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692023-02-011010.3389/fvets.2023.11166951116695Lactate is useful for the efficient replication of porcine epidemic diarrhea virus in cell cultureNile Wuri0Nile Wuri1Hongchao Gou2Hongchao Gou3Bin Zhang4Bin Zhang5Menglu Wang6Menglu Wang7Songqi Wang8Songqi Wang9Weixiao Zhang10Weixiao Zhang11Haiyan He12Haiyan He13Xuelei Fan14Xuelei Fan15Chunhong Zhang16Chunhong Zhang17Zhicheng Liu18Zhicheng Liu19Letu Geri20Haiyan Shen21Haiyan Shen22Jianfeng Zhang23Jianfeng Zhang24Key Laboratory of Livestock Disease Prevention of Guangdong Province, Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Ministry of Agriculture and Rural Affairs, Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou, ChinaCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, ChinaKey Laboratory of Livestock Disease Prevention of Guangdong Province, Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Ministry of Agriculture and Rural Affairs, Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou, ChinaMaoming Branch Center of Guangdong Laboratory for Lingnan Modern Agricultural Science and Technology, Maoming, ChinaKey Laboratory of Livestock Disease Prevention of Guangdong Province, Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Ministry of Agriculture and Rural Affairs, Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou, ChinaCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, ChinaKey Laboratory of Livestock Disease Prevention of Guangdong Province, Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Ministry of Agriculture and Rural Affairs, Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaKey Laboratory of Livestock Disease Prevention of Guangdong Province, Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Ministry of Agriculture and Rural Affairs, Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaKey Laboratory of Livestock Disease Prevention of Guangdong Province, Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Ministry of Agriculture and Rural Affairs, Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaKey Laboratory of Livestock Disease Prevention of Guangdong Province, Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Ministry of Agriculture and Rural Affairs, Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou, ChinaCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, ChinaKey Laboratory of Livestock Disease Prevention of Guangdong Province, Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Ministry of Agriculture and Rural Affairs, Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaKey Laboratory of Livestock Disease Prevention of Guangdong Province, Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Ministry of Agriculture and Rural Affairs, Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou, ChinaMaoming Branch Center of Guangdong Laboratory for Lingnan Modern Agricultural Science and Technology, Maoming, ChinaKey Laboratory of Livestock Disease Prevention of Guangdong Province, Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Ministry of Agriculture and Rural Affairs, Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou, ChinaMaoming Branch Center of Guangdong Laboratory for Lingnan Modern Agricultural Science and Technology, Maoming, ChinaCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, ChinaKey Laboratory of Livestock Disease Prevention of Guangdong Province, Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Ministry of Agriculture and Rural Affairs, Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou, ChinaMaoming Branch Center of Guangdong Laboratory for Lingnan Modern Agricultural Science and Technology, Maoming, ChinaKey Laboratory of Livestock Disease Prevention of Guangdong Province, Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Ministry of Agriculture and Rural Affairs, Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou, ChinaMaoming Branch Center of Guangdong Laboratory for Lingnan Modern Agricultural Science and Technology, Maoming, ChinaPorcine epidemic diarrhea virus (PEDV) is a deadly pathogen infecting pig herds, and has caused significant economic losses around the world. Vaccination remains the most effective way of keeping the PEDV epidemic under control. Previous studies have shown that the host metabolism has a significant impact on viral replication. In this study, we have demonstrated that two substrates of metabolic pathway, glucose and glutamine, play a key role in PEDV replication. Interestingly, the boosting effect of these compounds toward viral replication appeared to be dose-independent. Furthermore, we found that lactate, which is a downstream metabolite, promotes PEDV replication, even when added in excess to the cell culture medium. Moreover, the role of lactate in promoting PEDV was independent of the genotype of PEDV and the multiplicity of infection (MOI). Our findings suggest that lactate is a promising candidate for use as a cell culture additive for promoting PEDV replication. It could improve the efficiency of vaccine production and provide the basis for designing novel antiviral strategies.https://www.frontiersin.org/articles/10.3389/fvets.2023.1116695/fullporcine epidemic diarrhea virusmetabolitesglucoseglutaminelactate |
| spellingShingle | Nile Wuri Nile Wuri Hongchao Gou Hongchao Gou Bin Zhang Bin Zhang Menglu Wang Menglu Wang Songqi Wang Songqi Wang Weixiao Zhang Weixiao Zhang Haiyan He Haiyan He Xuelei Fan Xuelei Fan Chunhong Zhang Chunhong Zhang Zhicheng Liu Zhicheng Liu Letu Geri Haiyan Shen Haiyan Shen Jianfeng Zhang Jianfeng Zhang Lactate is useful for the efficient replication of porcine epidemic diarrhea virus in cell culture Frontiers in Veterinary Science porcine epidemic diarrhea virus metabolites glucose glutamine lactate |
| title | Lactate is useful for the efficient replication of porcine epidemic diarrhea virus in cell culture |
| title_full | Lactate is useful for the efficient replication of porcine epidemic diarrhea virus in cell culture |
| title_fullStr | Lactate is useful for the efficient replication of porcine epidemic diarrhea virus in cell culture |
| title_full_unstemmed | Lactate is useful for the efficient replication of porcine epidemic diarrhea virus in cell culture |
| title_short | Lactate is useful for the efficient replication of porcine epidemic diarrhea virus in cell culture |
| title_sort | lactate is useful for the efficient replication of porcine epidemic diarrhea virus in cell culture |
| topic | porcine epidemic diarrhea virus metabolites glucose glutamine lactate |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2023.1116695/full |
| work_keys_str_mv | AT nilewuri lactateisusefulfortheefficientreplicationofporcineepidemicdiarrheavirusincellculture AT nilewuri lactateisusefulfortheefficientreplicationofporcineepidemicdiarrheavirusincellculture AT hongchaogou lactateisusefulfortheefficientreplicationofporcineepidemicdiarrheavirusincellculture AT hongchaogou lactateisusefulfortheefficientreplicationofporcineepidemicdiarrheavirusincellculture AT binzhang lactateisusefulfortheefficientreplicationofporcineepidemicdiarrheavirusincellculture AT binzhang lactateisusefulfortheefficientreplicationofporcineepidemicdiarrheavirusincellculture AT mengluwang lactateisusefulfortheefficientreplicationofporcineepidemicdiarrheavirusincellculture AT mengluwang lactateisusefulfortheefficientreplicationofporcineepidemicdiarrheavirusincellculture AT songqiwang lactateisusefulfortheefficientreplicationofporcineepidemicdiarrheavirusincellculture AT songqiwang lactateisusefulfortheefficientreplicationofporcineepidemicdiarrheavirusincellculture AT weixiaozhang lactateisusefulfortheefficientreplicationofporcineepidemicdiarrheavirusincellculture AT weixiaozhang lactateisusefulfortheefficientreplicationofporcineepidemicdiarrheavirusincellculture AT haiyanhe lactateisusefulfortheefficientreplicationofporcineepidemicdiarrheavirusincellculture AT haiyanhe lactateisusefulfortheefficientreplicationofporcineepidemicdiarrheavirusincellculture AT xueleifan lactateisusefulfortheefficientreplicationofporcineepidemicdiarrheavirusincellculture AT xueleifan lactateisusefulfortheefficientreplicationofporcineepidemicdiarrheavirusincellculture AT chunhongzhang lactateisusefulfortheefficientreplicationofporcineepidemicdiarrheavirusincellculture AT chunhongzhang lactateisusefulfortheefficientreplicationofporcineepidemicdiarrheavirusincellculture AT zhichengliu lactateisusefulfortheefficientreplicationofporcineepidemicdiarrheavirusincellculture AT zhichengliu lactateisusefulfortheefficientreplicationofporcineepidemicdiarrheavirusincellculture AT letugeri lactateisusefulfortheefficientreplicationofporcineepidemicdiarrheavirusincellculture AT haiyanshen lactateisusefulfortheefficientreplicationofporcineepidemicdiarrheavirusincellculture AT haiyanshen lactateisusefulfortheefficientreplicationofporcineepidemicdiarrheavirusincellculture AT jianfengzhang lactateisusefulfortheefficientreplicationofporcineepidemicdiarrheavirusincellculture AT jianfengzhang lactateisusefulfortheefficientreplicationofporcineepidemicdiarrheavirusincellculture |