Vitreous Olink proteomics reveals inflammatory biomarkers for diagnosis and prognosis of traumatic proliferative vitreoretinopathy
BackgroundThe aim of this study was to identify inflammatory biomarkers in traumatic proliferative vitreoretinopathy (TPVR) patients and further validate the expression curve of particular biomarkers in the rabbit TPVR model.MethodsThe Olink Inflammation Panel was used to compare the differentially...
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Frontiers Media S.A.
2024-02-01
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2024.1355314/full |
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| author | Haixia Guo Tian Wang Tian Wang Jinguo Yu Zhemin Shi Minghui Liang Minghui Liang Minghui Liang Siyue Chen Siyue Chen Tiangeng He Hua Yan Hua Yan Hua Yan |
| author_facet | Haixia Guo Tian Wang Tian Wang Jinguo Yu Zhemin Shi Minghui Liang Minghui Liang Minghui Liang Siyue Chen Siyue Chen Tiangeng He Hua Yan Hua Yan Hua Yan |
| author_sort | Haixia Guo |
| collection | DOAJ |
| description | BackgroundThe aim of this study was to identify inflammatory biomarkers in traumatic proliferative vitreoretinopathy (TPVR) patients and further validate the expression curve of particular biomarkers in the rabbit TPVR model.MethodsThe Olink Inflammation Panel was used to compare the differentially expressed proteins (DEPs) in the vitreous of TPVR patients 7–14 days after open globe injury (OGI) (N = 19) and macular hole patients (N = 22), followed by correlation analysis between DEPs and clinical signs, protein–protein interaction (PPI) analysis, area under the receiver operating characteristic curve (AUC) analysis, and function enrichment analysis. A TPVR rabbit model was established and expression levels of candidate interleukin family members (IL-6, IL-7, and IL-33) were measured by enzyme-linked immunosorbent assay (ELISA) at 0, 1, 3, 7, 10, 14, and 28 days after OGI.ResultsForty-eight DEPs were detected between the two groups. Correlation analysis showed that CXCL5, EN-RAGE, IL-7, ADA, CD5, CCL25, CASP8, TWEAK, and IL-33 were significantly correlated with clinical signs including ocular wound characteristics, PVR scoring, PVR recurrence, and final visual acuity (R = 0.467–0.699, p < 0.05), and all with optimal AUC values (0.7344–1). Correlations between DEP analysis and PPI analysis further verified that IL-6, IL-7, IL-8, IL-33, HGF, and CXCL5 were highly interactive (combined score: 0.669–0.983). These DEPs were enriched in novel pathways such as cancer signaling pathway (N = 14, p < 0.000). Vitreous levels of IL-6, IL-7, and IL-33 in the rabbit TPVR model displayed consistency with the trend in Olink data, all exhibiting marked differential expression 1 day following the OGI.ConclusionIL-7, IL-33, EN-RAGE, TWEAK, CXCL5, and CD5 may be potential biomarkers for TPVR pathogenesis and prognosis, and early post-injury may be an ideal time for TPVR intervention targeting interleukin family biomarkers. |
| first_indexed | 2024-03-07T23:05:09Z |
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| id | doaj.art-723cd18e21b54a658c0c6bff15e75c45 |
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| last_indexed | 2024-03-07T23:05:09Z |
| publishDate | 2024-02-01 |
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| spelling | doaj.art-723cd18e21b54a658c0c6bff15e75c452024-02-22T05:18:37ZengFrontiers Media S.A.Frontiers in Immunology1664-32242024-02-011510.3389/fimmu.2024.13553141355314Vitreous Olink proteomics reveals inflammatory biomarkers for diagnosis and prognosis of traumatic proliferative vitreoretinopathyHaixia Guo0Tian Wang1Tian Wang2Jinguo Yu3Zhemin Shi4Minghui Liang5Minghui Liang6Minghui Liang7Siyue Chen8Siyue Chen9Tiangeng He10Hua Yan11Hua Yan12Hua Yan13Department of Ophthalmology, Tianjin Medical University General Hospital, Tianjin, ChinaShaanxi Eye Hospital, Xi’an People’s Hospital (Xi’an Fourth Hospital), Affiliated People’s Hospital of Northwest University, Xi’an, Shaanxi, ChinaInstitute of Medical Research, Northwestern Polytechnical University, Xi’an, Shaanxi, ChinaDepartment of Ophthalmology, Tianjin Medical University General Hospital, Tianjin, ChinaDepartment of Histology and Developmental Biology, Tianjin Medical University, Tianjin, ChinaDepartment of Ophthalmology, Tianjin Medical University General Hospital, Tianjin, ChinaTianjin Key Laboratory of Ocular Trauma, Laboratory of Molecular Ophthalmology, Tianjin Medical University, Tianjin, ChinaSchool of Medicine, Nankai University, Tianjin, ChinaDepartment of Ophthalmology, Tianjin Medical University General Hospital, Tianjin, ChinaTianjin Key Laboratory of Ocular Trauma, Laboratory of Molecular Ophthalmology, Tianjin Medical University, Tianjin, ChinaDepartment of Ophthalmology, Tianjin Medical University General Hospital, Tianjin, ChinaDepartment of Ophthalmology, Tianjin Medical University General Hospital, Tianjin, ChinaTianjin Key Laboratory of Ocular Trauma, Laboratory of Molecular Ophthalmology, Tianjin Medical University, Tianjin, ChinaSchool of Medicine, Nankai University, Tianjin, ChinaBackgroundThe aim of this study was to identify inflammatory biomarkers in traumatic proliferative vitreoretinopathy (TPVR) patients and further validate the expression curve of particular biomarkers in the rabbit TPVR model.MethodsThe Olink Inflammation Panel was used to compare the differentially expressed proteins (DEPs) in the vitreous of TPVR patients 7–14 days after open globe injury (OGI) (N = 19) and macular hole patients (N = 22), followed by correlation analysis between DEPs and clinical signs, protein–protein interaction (PPI) analysis, area under the receiver operating characteristic curve (AUC) analysis, and function enrichment analysis. A TPVR rabbit model was established and expression levels of candidate interleukin family members (IL-6, IL-7, and IL-33) were measured by enzyme-linked immunosorbent assay (ELISA) at 0, 1, 3, 7, 10, 14, and 28 days after OGI.ResultsForty-eight DEPs were detected between the two groups. Correlation analysis showed that CXCL5, EN-RAGE, IL-7, ADA, CD5, CCL25, CASP8, TWEAK, and IL-33 were significantly correlated with clinical signs including ocular wound characteristics, PVR scoring, PVR recurrence, and final visual acuity (R = 0.467–0.699, p < 0.05), and all with optimal AUC values (0.7344–1). Correlations between DEP analysis and PPI analysis further verified that IL-6, IL-7, IL-8, IL-33, HGF, and CXCL5 were highly interactive (combined score: 0.669–0.983). These DEPs were enriched in novel pathways such as cancer signaling pathway (N = 14, p < 0.000). Vitreous levels of IL-6, IL-7, and IL-33 in the rabbit TPVR model displayed consistency with the trend in Olink data, all exhibiting marked differential expression 1 day following the OGI.ConclusionIL-7, IL-33, EN-RAGE, TWEAK, CXCL5, and CD5 may be potential biomarkers for TPVR pathogenesis and prognosis, and early post-injury may be an ideal time for TPVR intervention targeting interleukin family biomarkers.https://www.frontiersin.org/articles/10.3389/fimmu.2024.1355314/fulltraumatic proliferative vitreoretinopathyinflammationOlinkbiomarkersinterleukinchemokine |
| spellingShingle | Haixia Guo Tian Wang Tian Wang Jinguo Yu Zhemin Shi Minghui Liang Minghui Liang Minghui Liang Siyue Chen Siyue Chen Tiangeng He Hua Yan Hua Yan Hua Yan Vitreous Olink proteomics reveals inflammatory biomarkers for diagnosis and prognosis of traumatic proliferative vitreoretinopathy Frontiers in Immunology traumatic proliferative vitreoretinopathy inflammation Olink biomarkers interleukin chemokine |
| title | Vitreous Olink proteomics reveals inflammatory biomarkers for diagnosis and prognosis of traumatic proliferative vitreoretinopathy |
| title_full | Vitreous Olink proteomics reveals inflammatory biomarkers for diagnosis and prognosis of traumatic proliferative vitreoretinopathy |
| title_fullStr | Vitreous Olink proteomics reveals inflammatory biomarkers for diagnosis and prognosis of traumatic proliferative vitreoretinopathy |
| title_full_unstemmed | Vitreous Olink proteomics reveals inflammatory biomarkers for diagnosis and prognosis of traumatic proliferative vitreoretinopathy |
| title_short | Vitreous Olink proteomics reveals inflammatory biomarkers for diagnosis and prognosis of traumatic proliferative vitreoretinopathy |
| title_sort | vitreous olink proteomics reveals inflammatory biomarkers for diagnosis and prognosis of traumatic proliferative vitreoretinopathy |
| topic | traumatic proliferative vitreoretinopathy inflammation Olink biomarkers interleukin chemokine |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2024.1355314/full |
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