COVID-19: molecular and serological detection methods
Since COVID-19, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), was declared as a pandemic disease by the World Health Organization in early 2020, many countries, organizations and companies have tried to find the best way to diagnose the virus and contain its spreading. SARS...
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| Format: | Article |
| Language: | English |
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PeerJ Inc.
2020-10-01
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| Series: | PeerJ |
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| Online Access: | https://peerj.com/articles/10180.pdf |
| _version_ | 1797417798397329408 |
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| author | Ahmed E. Dhamad Muna A. Abdal Rhida |
| author_facet | Ahmed E. Dhamad Muna A. Abdal Rhida |
| author_sort | Ahmed E. Dhamad |
| collection | DOAJ |
| description | Since COVID-19, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), was declared as a pandemic disease by the World Health Organization in early 2020, many countries, organizations and companies have tried to find the best way to diagnose the virus and contain its spreading. SARS-CoV-2 is a positive-sense single RNA (+ssRNA) coronavirus and mainly spreads through droplets, respiratory secretions, and direct contact. The early detection of the virus plays a central role in lowering COVID19 incidents and mortality rates. Thus, finding a simple, accurate, cheap and quick detection approach for SARS-CoV-2 at early stage of the viral infection is urgent and at high demand all around the world. The Food and Drug Administration and other health agencies have declared Emergency Use Authorization to develop diagnostic methods for COVID-19 and fulfill the demand. However, not all developed methods are appropriate and selecting a suitable method is challenging. Among all detection methods, rRT-PCR is the gold standard method. Unlike molecular methods, serological methods lack the ability of early detection with low accuracy. In this review, we summarized the current knowledge about COVID-19 detection methods aiming to highlight the advantages and disadvantages of molecular and serological methods. |
| first_indexed | 2024-03-09T06:23:52Z |
| format | Article |
| id | doaj.art-723d01ed6d2844ccbdf9ca5746b9ab78 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:23:52Z |
| publishDate | 2020-10-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-723d01ed6d2844ccbdf9ca5746b9ab782023-12-03T11:29:41ZengPeerJ Inc.PeerJ2167-83592020-10-018e1018010.7717/peerj.10180COVID-19: molecular and serological detection methodsAhmed E. Dhamad0Muna A. Abdal Rhida1Cell and Molecular Biology, University of Arkansas at Fayetteville, Fayetteville, AR, USACell and Molecular Biology, University of Arkansas at Fayetteville, Fayetteville, AR, USASince COVID-19, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), was declared as a pandemic disease by the World Health Organization in early 2020, many countries, organizations and companies have tried to find the best way to diagnose the virus and contain its spreading. SARS-CoV-2 is a positive-sense single RNA (+ssRNA) coronavirus and mainly spreads through droplets, respiratory secretions, and direct contact. The early detection of the virus plays a central role in lowering COVID19 incidents and mortality rates. Thus, finding a simple, accurate, cheap and quick detection approach for SARS-CoV-2 at early stage of the viral infection is urgent and at high demand all around the world. The Food and Drug Administration and other health agencies have declared Emergency Use Authorization to develop diagnostic methods for COVID-19 and fulfill the demand. However, not all developed methods are appropriate and selecting a suitable method is challenging. Among all detection methods, rRT-PCR is the gold standard method. Unlike molecular methods, serological methods lack the ability of early detection with low accuracy. In this review, we summarized the current knowledge about COVID-19 detection methods aiming to highlight the advantages and disadvantages of molecular and serological methods.https://peerj.com/articles/10180.pdfCOVID-19SARS-CoV-2rRT-PCRID NOW COVID-19Isothermal amplificationCRISPR-Cas12 |
| spellingShingle | Ahmed E. Dhamad Muna A. Abdal Rhida COVID-19: molecular and serological detection methods PeerJ COVID-19 SARS-CoV-2 rRT-PCR ID NOW COVID-19 Isothermal amplification CRISPR-Cas12 |
| title | COVID-19: molecular and serological detection methods |
| title_full | COVID-19: molecular and serological detection methods |
| title_fullStr | COVID-19: molecular and serological detection methods |
| title_full_unstemmed | COVID-19: molecular and serological detection methods |
| title_short | COVID-19: molecular and serological detection methods |
| title_sort | covid 19 molecular and serological detection methods |
| topic | COVID-19 SARS-CoV-2 rRT-PCR ID NOW COVID-19 Isothermal amplification CRISPR-Cas12 |
| url | https://peerj.com/articles/10180.pdf |
| work_keys_str_mv | AT ahmededhamad covid19molecularandserologicaldetectionmethods AT munaaabdalrhida covid19molecularandserologicaldetectionmethods |