Enhancement of Carbon Conversion and Value-Added Compound Production in Heterotrophic <i>Chlorella vulgaris</i> Using Sweet Sorghum Extract
High-cost carbon sources are not economical or sustainable for the heterotrophic culture of <i>Chlorella vulgaris</i>. In order to reduce the cost, this study used sweet sorghum extract (SE) and its enzymatic hydrolysate (HSE) as alternative carbon sources for the heterotrophic culture o...
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| Format: | Article |
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MDPI AG
2022-08-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/11/17/2579 |
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| author | Kangping Wu Yilin Fang Biyuan Hong Yihui Cai Honglei Xie Yunpu Wang Xian Cui Zhigang Yu Yuhuan Liu Roger Ruan Qi Zhang |
| author_facet | Kangping Wu Yilin Fang Biyuan Hong Yihui Cai Honglei Xie Yunpu Wang Xian Cui Zhigang Yu Yuhuan Liu Roger Ruan Qi Zhang |
| author_sort | Kangping Wu |
| collection | DOAJ |
| description | High-cost carbon sources are not economical or sustainable for the heterotrophic culture of <i>Chlorella vulgaris</i>. In order to reduce the cost, this study used sweet sorghum extract (SE) and its enzymatic hydrolysate (HSE) as alternative carbon sources for the heterotrophic culture of <i>Chlorella vulgaris</i>. Under the premise of the same total carbon concentration, the value-added product production performance of <i>Chlorella vulgaris</i> cultured in HSE (supplemented with nitrogen sources and minerals) was much better than that in the glucose medium. The conversion rate of the total organic carbon and the utilization rate of the total nitrogen were both improved in the HSE system. The biomass production and productivity using HSE reached 2.51 g/L and 0.42 g/L/d, respectively. The production of proteins and lipids using HSE reached 1.17 and 0.35 g/L, respectively, and the production of chlorophyll-a, carotenoid, and lutein using HSE reached 30.42, 10.99, and 0.88 mg/L, respectively. The medium cost using HSE decreased by 69.61% compared to glucose. This study proves the feasibility and practicability of using HSE as a carbon source for the low-cost heterotrophic culture of <i>Chlorella vulgaris</i>. |
| first_indexed | 2024-03-10T01:50:05Z |
| format | Article |
| id | doaj.art-7240fd440f6a4caab21d15dd9eda6397 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T01:50:05Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-7240fd440f6a4caab21d15dd9eda63972023-11-23T13:07:44ZengMDPI AGFoods2304-81582022-08-011117257910.3390/foods11172579Enhancement of Carbon Conversion and Value-Added Compound Production in Heterotrophic <i>Chlorella vulgaris</i> Using Sweet Sorghum ExtractKangping Wu0Yilin Fang1Biyuan Hong2Yihui Cai3Honglei Xie4Yunpu Wang5Xian Cui6Zhigang Yu7Yuhuan Liu8Roger Ruan9Qi Zhang10State Key Laboratory of Food Science and Technology, Engineering Research Center for Biomass Conversion, Ministry of Education, College of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaQueen Mary School, Jiangxi Medical College, Nanchang University, Nanchang 330047, ChinaChina Coal Zhejiang Testing Technology Co., Ltd., Hangzhou 310000, ChinaState Key Laboratory of Food Science and Technology, Engineering Research Center for Biomass Conversion, Ministry of Education, College of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaHarbin Center for Disease Control and Prevention, Harbin 150000, ChinaState Key Laboratory of Food Science and Technology, Engineering Research Center for Biomass Conversion, Ministry of Education, College of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Technology, Engineering Research Center for Biomass Conversion, Ministry of Education, College of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaAustralian Centre for Water and Environmental Biotechnology (Formerly AWMC), The University of Queensland, St. Lucia, Brisbane, QLD 4072, AustraliaState Key Laboratory of Food Science and Technology, Engineering Research Center for Biomass Conversion, Ministry of Education, College of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaCenter for Biorefining, Department of Bioproducts and Biosystems Engineering and Department of Food Science and Nutrition, University of Minnesota, Saint Paul, MN 55108, USAState Key Laboratory of Food Science and Technology, Engineering Research Center for Biomass Conversion, Ministry of Education, College of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaHigh-cost carbon sources are not economical or sustainable for the heterotrophic culture of <i>Chlorella vulgaris</i>. In order to reduce the cost, this study used sweet sorghum extract (SE) and its enzymatic hydrolysate (HSE) as alternative carbon sources for the heterotrophic culture of <i>Chlorella vulgaris</i>. Under the premise of the same total carbon concentration, the value-added product production performance of <i>Chlorella vulgaris</i> cultured in HSE (supplemented with nitrogen sources and minerals) was much better than that in the glucose medium. The conversion rate of the total organic carbon and the utilization rate of the total nitrogen were both improved in the HSE system. The biomass production and productivity using HSE reached 2.51 g/L and 0.42 g/L/d, respectively. The production of proteins and lipids using HSE reached 1.17 and 0.35 g/L, respectively, and the production of chlorophyll-a, carotenoid, and lutein using HSE reached 30.42, 10.99, and 0.88 mg/L, respectively. The medium cost using HSE decreased by 69.61% compared to glucose. This study proves the feasibility and practicability of using HSE as a carbon source for the low-cost heterotrophic culture of <i>Chlorella vulgaris</i>.https://www.mdpi.com/2304-8158/11/17/2579<i>Chlorella vulgaris</i>sweet sorghumcarbon sourcebiomass productionnutrient utilization |
| spellingShingle | Kangping Wu Yilin Fang Biyuan Hong Yihui Cai Honglei Xie Yunpu Wang Xian Cui Zhigang Yu Yuhuan Liu Roger Ruan Qi Zhang Enhancement of Carbon Conversion and Value-Added Compound Production in Heterotrophic <i>Chlorella vulgaris</i> Using Sweet Sorghum Extract Foods <i>Chlorella vulgaris</i> sweet sorghum carbon source biomass production nutrient utilization |
| title | Enhancement of Carbon Conversion and Value-Added Compound Production in Heterotrophic <i>Chlorella vulgaris</i> Using Sweet Sorghum Extract |
| title_full | Enhancement of Carbon Conversion and Value-Added Compound Production in Heterotrophic <i>Chlorella vulgaris</i> Using Sweet Sorghum Extract |
| title_fullStr | Enhancement of Carbon Conversion and Value-Added Compound Production in Heterotrophic <i>Chlorella vulgaris</i> Using Sweet Sorghum Extract |
| title_full_unstemmed | Enhancement of Carbon Conversion and Value-Added Compound Production in Heterotrophic <i>Chlorella vulgaris</i> Using Sweet Sorghum Extract |
| title_short | Enhancement of Carbon Conversion and Value-Added Compound Production in Heterotrophic <i>Chlorella vulgaris</i> Using Sweet Sorghum Extract |
| title_sort | enhancement of carbon conversion and value added compound production in heterotrophic i chlorella vulgaris i using sweet sorghum extract |
| topic | <i>Chlorella vulgaris</i> sweet sorghum carbon source biomass production nutrient utilization |
| url | https://www.mdpi.com/2304-8158/11/17/2579 |
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