Transcriptome analysis in LRRK2 and idiopathic Parkinson’s disease at different glucose levels

Abstract Type-2 diabetes (T2D) and glucose metabolic imbalances have been linked to neurodegenerative diseases, including Parkinson’s disease (PD). To detect potential effects of different glucose levels on gene expression, by RNA-seq we analyzed the transcriptome of dermal fibroblasts from idiopath...

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Main Authors: Rubén Fernández-Santiago, Anna Esteve-Codina, Manel Fernández, Francesc Valldeoriola, Almudena Sanchez-Gómez, Esteban Muñoz, Yaroslau Compta, Eduardo Tolosa, Mario Ezquerra, María J. Martí
Format: Article
Language:English
Published: Nature Portfolio 2021-12-01
Series:npj Parkinson's Disease
Online Access:https://doi.org/10.1038/s41531-021-00255-x
Description
Summary:Abstract Type-2 diabetes (T2D) and glucose metabolic imbalances have been linked to neurodegenerative diseases, including Parkinson’s disease (PD). To detect potential effects of different glucose levels on gene expression, by RNA-seq we analyzed the transcriptome of dermal fibroblasts from idiopathic PD (iPD) patients, LRRK2-associated PD (L2PD) patients, and healthy controls (total n = 21 cell lines), which were cultured at two different glucose concentrations (25 and 5 mM glucose). In PD patients we identified differentially expressed genes (DEGs) that were related to biological processes mainly involving the plasmatic cell membrane, the extracellular matrix, and also neuronal functions. Such pathway deregulation was largely similar in iPD or L2PD fibroblasts. Overall, the gene expression changes detected in this study were associated with PD independently of glucose concentration.
ISSN:2373-8057