Localization of the Elastic Proteins in the Flight Muscle of <i>Manduca sexta</i>
The flight muscle of <i>Manduca sexta</i> (DLM<sub>1</sub>) is an emerging model system for biophysical studies of muscle contraction. Unlike the well-studied indirect flight muscle of <i>Lethocerus</i> and <i>Drosophila</i>, the DLM<sub>1</su...
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MDPI AG
2020-07-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/15/5504 |
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| author | Henry Gong Weikang Ma Shaoshuai Chen Geng Wang Ramzi Khairallah Thomas Irving |
| author_facet | Henry Gong Weikang Ma Shaoshuai Chen Geng Wang Ramzi Khairallah Thomas Irving |
| author_sort | Henry Gong |
| collection | DOAJ |
| description | The flight muscle of <i>Manduca sexta</i> (DLM<sub>1</sub>) is an emerging model system for biophysical studies of muscle contraction. Unlike the well-studied indirect flight muscle of <i>Lethocerus</i> and <i>Drosophila</i>, the DLM<sub>1</sub> of <i>Manduca</i> is a synchronous muscle, as are the vertebrate cardiac and skeletal muscles. Very little has been published regarding the ultrastructure and protein composition of this muscle. Previous studies have demonstrated that DLM<sub>1</sub> express two projectin isoform, two kettin isoforms, and two large Salimus (Sls) isoforms. Such large Sls isoforms have not been observed in the asynchronous flight muscles of <i>Lethocerus</i> and <i>Drosophila.</i> The spatial localization of these proteins was unknown. Here, immuno-localization was used to show that the N-termini of projectin and Salimus are inserted into the Z-band. Projectin spans across the I-band, and the C-terminus is attached to the thick filament in the A-band. The C-terminus of Sls was also located in the A-band. Using confocal microscopy and experimental force-length curves, thin filament lengths were estimated as ~1.5 µm and thick filament lengths were measured as ~2.5 µm. This structural information may help provide an interpretive framework for future studies using this muscle system. |
| first_indexed | 2024-03-10T18:03:09Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T18:03:09Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-7247f13e2a7c44ddbd5fddcf286cf0542023-11-20T08:43:07ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-07-012115550410.3390/ijms21155504Localization of the Elastic Proteins in the Flight Muscle of <i>Manduca sexta</i>Henry Gong0Weikang Ma1Shaoshuai Chen2Geng Wang3Ramzi Khairallah4Thomas Irving5Department of Biological Sciences, Illinois Institute of Technology, Chicago, IL 60616, USADepartment of Biological Sciences, Illinois Institute of Technology, Chicago, IL 60616, USADepartment of Biological Sciences, Illinois Institute of Technology, Chicago, IL 60616, USADepartment of Biological Sciences, Illinois Institute of Technology, Chicago, IL 60616, USADepartment of Cell and Molecular Physiology, Loyola University Chicago, Stritch School of Medicine, Maywood, IL 60153, USADepartment of Biological Sciences, Illinois Institute of Technology, Chicago, IL 60616, USAThe flight muscle of <i>Manduca sexta</i> (DLM<sub>1</sub>) is an emerging model system for biophysical studies of muscle contraction. Unlike the well-studied indirect flight muscle of <i>Lethocerus</i> and <i>Drosophila</i>, the DLM<sub>1</sub> of <i>Manduca</i> is a synchronous muscle, as are the vertebrate cardiac and skeletal muscles. Very little has been published regarding the ultrastructure and protein composition of this muscle. Previous studies have demonstrated that DLM<sub>1</sub> express two projectin isoform, two kettin isoforms, and two large Salimus (Sls) isoforms. Such large Sls isoforms have not been observed in the asynchronous flight muscles of <i>Lethocerus</i> and <i>Drosophila.</i> The spatial localization of these proteins was unknown. Here, immuno-localization was used to show that the N-termini of projectin and Salimus are inserted into the Z-band. Projectin spans across the I-band, and the C-terminus is attached to the thick filament in the A-band. The C-terminus of Sls was also located in the A-band. Using confocal microscopy and experimental force-length curves, thin filament lengths were estimated as ~1.5 µm and thick filament lengths were measured as ~2.5 µm. This structural information may help provide an interpretive framework for future studies using this muscle system.https://www.mdpi.com/1422-0067/21/15/5504<i>Manduca sexta</i>synchronous insect flight musclemyofilament lengthselastic proteinssarcomere structures |
| spellingShingle | Henry Gong Weikang Ma Shaoshuai Chen Geng Wang Ramzi Khairallah Thomas Irving Localization of the Elastic Proteins in the Flight Muscle of <i>Manduca sexta</i> International Journal of Molecular Sciences <i>Manduca sexta</i> synchronous insect flight muscle myofilament lengths elastic proteins sarcomere structures |
| title | Localization of the Elastic Proteins in the Flight Muscle of <i>Manduca sexta</i> |
| title_full | Localization of the Elastic Proteins in the Flight Muscle of <i>Manduca sexta</i> |
| title_fullStr | Localization of the Elastic Proteins in the Flight Muscle of <i>Manduca sexta</i> |
| title_full_unstemmed | Localization of the Elastic Proteins in the Flight Muscle of <i>Manduca sexta</i> |
| title_short | Localization of the Elastic Proteins in the Flight Muscle of <i>Manduca sexta</i> |
| title_sort | localization of the elastic proteins in the flight muscle of i manduca sexta i |
| topic | <i>Manduca sexta</i> synchronous insect flight muscle myofilament lengths elastic proteins sarcomere structures |
| url | https://www.mdpi.com/1422-0067/21/15/5504 |
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