Plant Growth Promoting Activities of Spore-Forming and Vegetative Cells of Salt-Tolerant Rhizobacteria under Salinity Condition
Plant growth promoting rhizobacteria are able to enhance plant growth. This study isolated spore-forming rhizobacteria from vegetable rhizosphere soil samples. These isolates were tested for their abilities to promote plant growth and to be used in bio-fertilizers. Thirty-nine isolates with differe...
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| Format: | Article |
| Language: | English |
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Mahidol University
2022-04-01
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| Series: | Environment and Natural Resources Journal |
| Subjects: | |
| Online Access: | https://ph02.tci-thaijo.org/index.php/ennrj/article/view/246115/166985 |
| _version_ | 1818544625120444416 |
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| author | Tiptida Kidtook Jindarat Ekprasert Nuntavun Riddech |
| author_facet | Tiptida Kidtook Jindarat Ekprasert Nuntavun Riddech |
| author_sort | Tiptida Kidtook |
| collection | DOAJ |
| description | Plant growth promoting rhizobacteria are able to enhance plant growth. This study isolated spore-forming rhizobacteria from vegetable rhizosphere soil samples. These isolates were tested for their abilities to promote plant growth and to be used in bio-fertilizers. Thirty-nine isolates with different characteristics were obtained. Three isolates, TYS1.1, TYS3.3, and TYS3.5, showed multifunctional activities on nitrogen fixation and potassium solubilization. They were tested for IAA production in liquid medium supplemented with tryptophan and NaCl, with the vegetative cells of isolate TYS3.5 showing the highest IAA production. The colonization of the three isolates on okra roots was checked by spread plate technique and scanning electron microscope. It was found that rhizobacteria could colonize plant roots with a concentration of 8.19 log CFU/g in the presence of 50 mM NaCl solution. Bio-fertilizer was produced by immobilizing the mixture of three isolates on carriers. The viable cells were enumerated during the storage at room temperature for 60 days. The results showed that the highest number of survival cells in the form of vegetative and spore-forming cells were obtained when using rice husk ash and vermiculite as a carrier. The concentration of viable cells was in a range of 8.14-8.44 log CFU/g. These isolates were Bacillus sp. according to the 16S rDNA sequencing analysis. |
| first_indexed | 2024-12-11T22:50:56Z |
| format | Article |
| id | doaj.art-72486c572254445c8a13228c4bf2927a |
| institution | Directory Open Access Journal |
| issn | 1686-5456 2408-2384 |
| language | English |
| last_indexed | 2024-12-11T22:50:56Z |
| publishDate | 2022-04-01 |
| publisher | Mahidol University |
| record_format | Article |
| series | Environment and Natural Resources Journal |
| spelling | doaj.art-72486c572254445c8a13228c4bf2927a2022-12-22T00:47:28ZengMahidol UniversityEnvironment and Natural Resources Journal1686-54562408-23842022-04-0120325726510.32526/ennrj/20/202100211Plant Growth Promoting Activities of Spore-Forming and Vegetative Cells of Salt-Tolerant Rhizobacteria under Salinity ConditionTiptida Kidtook0Jindarat Ekprasert1Nuntavun Riddech2Graduate School of Microbiology Program, Department of Microbiology, Faculty of Science, Khon Kaen University, Khon Kaen 40002, ThailandDepartment of Microbiology, Faculty of Science, Khon Kaen University, Khon Kaen 40002, ThailandDepartment of Microbiology, Faculty of Science, Khon Kaen University, Khon Kaen 40002, ThailandPlant growth promoting rhizobacteria are able to enhance plant growth. This study isolated spore-forming rhizobacteria from vegetable rhizosphere soil samples. These isolates were tested for their abilities to promote plant growth and to be used in bio-fertilizers. Thirty-nine isolates with different characteristics were obtained. Three isolates, TYS1.1, TYS3.3, and TYS3.5, showed multifunctional activities on nitrogen fixation and potassium solubilization. They were tested for IAA production in liquid medium supplemented with tryptophan and NaCl, with the vegetative cells of isolate TYS3.5 showing the highest IAA production. The colonization of the three isolates on okra roots was checked by spread plate technique and scanning electron microscope. It was found that rhizobacteria could colonize plant roots with a concentration of 8.19 log CFU/g in the presence of 50 mM NaCl solution. Bio-fertilizer was produced by immobilizing the mixture of three isolates on carriers. The viable cells were enumerated during the storage at room temperature for 60 days. The results showed that the highest number of survival cells in the form of vegetative and spore-forming cells were obtained when using rice husk ash and vermiculite as a carrier. The concentration of viable cells was in a range of 8.14-8.44 log CFU/g. These isolates were Bacillus sp. according to the 16S rDNA sequencing analysis.https://ph02.tci-thaijo.org/index.php/ennrj/article/view/246115/166985spore forming rhizobacteriavegetative cell rhizobacteriasalinity condition |
| spellingShingle | Tiptida Kidtook Jindarat Ekprasert Nuntavun Riddech Plant Growth Promoting Activities of Spore-Forming and Vegetative Cells of Salt-Tolerant Rhizobacteria under Salinity Condition Environment and Natural Resources Journal spore forming rhizobacteria vegetative cell rhizobacteria salinity condition |
| title | Plant Growth Promoting Activities of Spore-Forming and Vegetative Cells of Salt-Tolerant Rhizobacteria under Salinity Condition |
| title_full | Plant Growth Promoting Activities of Spore-Forming and Vegetative Cells of Salt-Tolerant Rhizobacteria under Salinity Condition |
| title_fullStr | Plant Growth Promoting Activities of Spore-Forming and Vegetative Cells of Salt-Tolerant Rhizobacteria under Salinity Condition |
| title_full_unstemmed | Plant Growth Promoting Activities of Spore-Forming and Vegetative Cells of Salt-Tolerant Rhizobacteria under Salinity Condition |
| title_short | Plant Growth Promoting Activities of Spore-Forming and Vegetative Cells of Salt-Tolerant Rhizobacteria under Salinity Condition |
| title_sort | plant growth promoting activities of spore forming and vegetative cells of salt tolerant rhizobacteria under salinity condition |
| topic | spore forming rhizobacteria vegetative cell rhizobacteria salinity condition |
| url | https://ph02.tci-thaijo.org/index.php/ennrj/article/view/246115/166985 |
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