Inflammatory responses and intestinal injury development during acute Trypanosoma cruzi infection are associated with the parasite load
Abstract Background Chagas disease is caused by the protozoan Trypanosoma cruzi and is characterized by cardiac, gastrointestinal, and nervous system disorders. Although much about the pathophysiological process of Chagas disease is already known, the influence of the parasite burden on the inflamma...
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| Format: | Article |
| Language: | English |
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BMC
2015-04-01
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| Series: | Parasites & Vectors |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13071-015-0811-8 |
| _version_ | 1797811821954990080 |
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| author | Bruna Perez Vazquez Thaís Perez Vazquez Camila Botelho Miguel Wellington Francisco Rodrigues Maria Tays Mendes Carlo José Freire de Oliveira Javier Emílio Lazo Chica |
| author_facet | Bruna Perez Vazquez Thaís Perez Vazquez Camila Botelho Miguel Wellington Francisco Rodrigues Maria Tays Mendes Carlo José Freire de Oliveira Javier Emílio Lazo Chica |
| author_sort | Bruna Perez Vazquez |
| collection | DOAJ |
| description | Abstract Background Chagas disease is caused by the protozoan Trypanosoma cruzi and is characterized by cardiac, gastrointestinal, and nervous system disorders. Although much about the pathophysiological process of Chagas disease is already known, the influence of the parasite burden on the inflammatory process and disease progression remains uncertain. Methods We used an acute experimental disease model to evaluate the effect of T. cruzi on intestinal lesions and assessed correlations between parasite load and inflammation and intestinal injury at 7 and 14 days post-infection. Low (3 × 102), medium (3 × 103), and high (3 × 104) parasite loads were generated by infecting C57BL/6 mice with “Y”-strain trypomastigotes. Statistical analysis was performed using analysis of variance with Tukey’s multiple comparison post-test, Kruskal–Wallis test with Dunn’s multiple comparison, χ2 test and Spearman correlation. Results High parasite load-bearing mice more rapidly and strongly developed parasitemia. Increased colon width, inflammatory infiltration, myositis, periganglionitis, ganglionitis, pro-inflammatory cytokines (e.g., TNF-α, INF-γ, IL-2, IL-17, IL-6), and intestinal amastigote nests were more pronounced in high parasite load-bearing animals. These results were remarkable because a positive correlation was observed between parasite load, inflammatory infiltrate, amastigote nests, and investigated cytokines. Conclusions These experimental data support the idea that the parasite load considerably influences the T. cruzi-induced intestinal inflammatory response and contributes to the development of the digestive form of the disease. |
| first_indexed | 2024-03-13T07:29:29Z |
| format | Article |
| id | doaj.art-724e8099be56437593ec01424d5999fc |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-03-13T07:29:29Z |
| publishDate | 2015-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-724e8099be56437593ec01424d5999fc2023-06-04T11:11:39ZengBMCParasites & Vectors1756-33052015-04-018111210.1186/s13071-015-0811-8Inflammatory responses and intestinal injury development during acute Trypanosoma cruzi infection are associated with the parasite loadBruna Perez Vazquez0Thaís Perez Vazquez1Camila Botelho Miguel2Wellington Francisco Rodrigues3Maria Tays Mendes4Carlo José Freire de Oliveira5Javier Emílio Lazo Chica6Disciplina de Biologia Celular/Instituto de Ciências Biológicas e Naturais, Universidade Federal do Triângulo MineiroDisciplina de Biologia Celular/Instituto de Ciências Biológicas e Naturais, Universidade Federal do Triângulo MineiroCurso de Pós-graduação em Ciências da Saúde, Universidade Federal do Triângulo MineiroCurso de Pós-graduação em Ciências da Saúde, Universidade Federal do Triângulo MineiroCurso de Pós-Graduação em Medicina Tropical e Infectologia, Universidade Federal do Triângulo MineiroCurso de Pós-Graduação em Medicina Tropical e Infectologia, Universidade Federal do Triângulo MineiroCurso de Pós-graduação em Ciências da Saúde, Universidade Federal do Triângulo MineiroAbstract Background Chagas disease is caused by the protozoan Trypanosoma cruzi and is characterized by cardiac, gastrointestinal, and nervous system disorders. Although much about the pathophysiological process of Chagas disease is already known, the influence of the parasite burden on the inflammatory process and disease progression remains uncertain. Methods We used an acute experimental disease model to evaluate the effect of T. cruzi on intestinal lesions and assessed correlations between parasite load and inflammation and intestinal injury at 7 and 14 days post-infection. Low (3 × 102), medium (3 × 103), and high (3 × 104) parasite loads were generated by infecting C57BL/6 mice with “Y”-strain trypomastigotes. Statistical analysis was performed using analysis of variance with Tukey’s multiple comparison post-test, Kruskal–Wallis test with Dunn’s multiple comparison, χ2 test and Spearman correlation. Results High parasite load-bearing mice more rapidly and strongly developed parasitemia. Increased colon width, inflammatory infiltration, myositis, periganglionitis, ganglionitis, pro-inflammatory cytokines (e.g., TNF-α, INF-γ, IL-2, IL-17, IL-6), and intestinal amastigote nests were more pronounced in high parasite load-bearing animals. These results were remarkable because a positive correlation was observed between parasite load, inflammatory infiltrate, amastigote nests, and investigated cytokines. Conclusions These experimental data support the idea that the parasite load considerably influences the T. cruzi-induced intestinal inflammatory response and contributes to the development of the digestive form of the disease.https://doi.org/10.1186/s13071-015-0811-8T. CruziChagas diseaseParasite loadIntestinal injuryInflammation |
| spellingShingle | Bruna Perez Vazquez Thaís Perez Vazquez Camila Botelho Miguel Wellington Francisco Rodrigues Maria Tays Mendes Carlo José Freire de Oliveira Javier Emílio Lazo Chica Inflammatory responses and intestinal injury development during acute Trypanosoma cruzi infection are associated with the parasite load Parasites & Vectors T. Cruzi Chagas disease Parasite load Intestinal injury Inflammation |
| title | Inflammatory responses and intestinal injury development during acute Trypanosoma cruzi infection are associated with the parasite load |
| title_full | Inflammatory responses and intestinal injury development during acute Trypanosoma cruzi infection are associated with the parasite load |
| title_fullStr | Inflammatory responses and intestinal injury development during acute Trypanosoma cruzi infection are associated with the parasite load |
| title_full_unstemmed | Inflammatory responses and intestinal injury development during acute Trypanosoma cruzi infection are associated with the parasite load |
| title_short | Inflammatory responses and intestinal injury development during acute Trypanosoma cruzi infection are associated with the parasite load |
| title_sort | inflammatory responses and intestinal injury development during acute trypanosoma cruzi infection are associated with the parasite load |
| topic | T. Cruzi Chagas disease Parasite load Intestinal injury Inflammation |
| url | https://doi.org/10.1186/s13071-015-0811-8 |
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