Long-term in vivo imaging of multiple organs at the single cell level.
Two-photon microscopy has enabled the study of individual cell behavior in live animals. Many organs and tissues cannot be studied, especially longitudinally, because they are located too deep, behind bony structures or too close to the lung and heart. Here we report a novel mouse model that allows...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3534688?pdf=render |
| _version_ | 1818925777574428672 |
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| author | Benny J Chen Yiqun Jiao Ping Zhang Albert Y Sun Geoffrey S Pitt Divino Deoliveira Nicholas Drago Tong Ye Chen Liu Nelson J Chao |
| author_facet | Benny J Chen Yiqun Jiao Ping Zhang Albert Y Sun Geoffrey S Pitt Divino Deoliveira Nicholas Drago Tong Ye Chen Liu Nelson J Chao |
| author_sort | Benny J Chen |
| collection | DOAJ |
| description | Two-photon microscopy has enabled the study of individual cell behavior in live animals. Many organs and tissues cannot be studied, especially longitudinally, because they are located too deep, behind bony structures or too close to the lung and heart. Here we report a novel mouse model that allows long-term single cell imaging of many organs. A wide variety of live tissues were successfully engrafted in the pinna of the mouse ear. Many of these engrafted tissues maintained the normal tissue histology. Using the heart and thymus as models, we further demonstrated that the engrafted tissues functioned as would be expected. Combining two-photon microscopy with fluorescent tracers, we successfully visualized the engrafted tissues at the single cell level in live mice over several months. Four dimensional (three-dimensional (3D) plus time) information of individual cells was obtained from this imaging. This model makes long-term high resolution 4D imaging of multiple organs possible. |
| first_indexed | 2024-12-20T02:46:37Z |
| format | Article |
| id | doaj.art-725514fd8a0e416bb3e163d76a33976c |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T02:46:37Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-725514fd8a0e416bb3e163d76a33976c2022-12-21T19:56:09ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0181e5208710.1371/journal.pone.0052087Long-term in vivo imaging of multiple organs at the single cell level.Benny J ChenYiqun JiaoPing ZhangAlbert Y SunGeoffrey S PittDivino DeoliveiraNicholas DragoTong YeChen LiuNelson J ChaoTwo-photon microscopy has enabled the study of individual cell behavior in live animals. Many organs and tissues cannot be studied, especially longitudinally, because they are located too deep, behind bony structures or too close to the lung and heart. Here we report a novel mouse model that allows long-term single cell imaging of many organs. A wide variety of live tissues were successfully engrafted in the pinna of the mouse ear. Many of these engrafted tissues maintained the normal tissue histology. Using the heart and thymus as models, we further demonstrated that the engrafted tissues functioned as would be expected. Combining two-photon microscopy with fluorescent tracers, we successfully visualized the engrafted tissues at the single cell level in live mice over several months. Four dimensional (three-dimensional (3D) plus time) information of individual cells was obtained from this imaging. This model makes long-term high resolution 4D imaging of multiple organs possible.http://europepmc.org/articles/PMC3534688?pdf=render |
| spellingShingle | Benny J Chen Yiqun Jiao Ping Zhang Albert Y Sun Geoffrey S Pitt Divino Deoliveira Nicholas Drago Tong Ye Chen Liu Nelson J Chao Long-term in vivo imaging of multiple organs at the single cell level. PLoS ONE |
| title | Long-term in vivo imaging of multiple organs at the single cell level. |
| title_full | Long-term in vivo imaging of multiple organs at the single cell level. |
| title_fullStr | Long-term in vivo imaging of multiple organs at the single cell level. |
| title_full_unstemmed | Long-term in vivo imaging of multiple organs at the single cell level. |
| title_short | Long-term in vivo imaging of multiple organs at the single cell level. |
| title_sort | long term in vivo imaging of multiple organs at the single cell level |
| url | http://europepmc.org/articles/PMC3534688?pdf=render |
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