Molecular Characterization of Peripheral Extracellular Vesicles in Clinically Isolated Syndrome: Preliminary Suggestions from a Pilot Study
Extracellular vesicles (EVs), nanoparticles originated from different cell types, seem to be implicated in several cellular activities. In the Central Nervous System (CNS), glia and neurons secrete EVs and recent studies have demonstrated that the intercellular communication mediated by EVs has vers...
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MDPI AG
2017-09-01
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| Online Access: | https://www.mdpi.com/2076-3271/5/3/19 |
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| author | Nicoletta Nuzziello Maria Blonda Flavio Licciulli Sabino Liuni Antonella Amoruso Alessio Valletti Arianna Consiglio Carlo Avolio Maria Liguori |
| author_facet | Nicoletta Nuzziello Maria Blonda Flavio Licciulli Sabino Liuni Antonella Amoruso Alessio Valletti Arianna Consiglio Carlo Avolio Maria Liguori |
| author_sort | Nicoletta Nuzziello |
| collection | DOAJ |
| description | Extracellular vesicles (EVs), nanoparticles originated from different cell types, seem to be implicated in several cellular activities. In the Central Nervous System (CNS), glia and neurons secrete EVs and recent studies have demonstrated that the intercellular communication mediated by EVs has versatile functional impact in the cerebral homeostasis. This essential role may be due to their proteins and RNAs cargo that possibly modify the phenotypes of the targeted cells. Despite the increasing importance of EVs, little is known about their fluctuations in physiological as well as in pathological conditions. Furthermore, only few studies have investigated the contents of contemporary EVs subgroups (microvesicles, MVs and exosomes, EXOs) with the purpose of discriminating between their features and functional roles. In order to possibly shed light on these issues, we performed a pilot study in which MVs and EXOs extracted from serum samples of a little cohort of subjects (patients with the first clinical evidence of CNS demyelination, also known as Clinically Isolated Syndrome and Healthy Controls) were submitted to deep small-RNA sequencing. Data were analysed by an in-home bioinformatics platform. In line with previous reports, distinct classes of non-coding RNAs have been detected in both the EVs subsets, offering interesting suggestions on their origins and functions. We also verified the feasibility of this extensive molecular approach, thus supporting its valuable use for the analysis of circulating biomarkers (e.g., microRNAs) in order to investigate and monitor specific diseases. |
| first_indexed | 2024-04-13T00:32:26Z |
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| institution | Directory Open Access Journal |
| issn | 2076-3271 |
| language | English |
| last_indexed | 2024-04-13T00:32:26Z |
| publishDate | 2017-09-01 |
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| series | Medical Sciences |
| spelling | doaj.art-7258a0204f0b47a48013ba36529c6a4b2022-12-22T03:10:26ZengMDPI AGMedical Sciences2076-32712017-09-01531910.3390/medsci5030019medsci5030019Molecular Characterization of Peripheral Extracellular Vesicles in Clinically Isolated Syndrome: Preliminary Suggestions from a Pilot StudyNicoletta Nuzziello0Maria Blonda1Flavio Licciulli2Sabino Liuni3Antonella Amoruso4Alessio Valletti5Arianna Consiglio6Carlo Avolio7Maria Liguori8National Research Council of Italy, Institute of Biomedical Technologies, Section of Bari, via Amendola no. 122/D, 70126 Bari, ItalyDepartment of Medical and Surgical Sciences, University of Foggia, 71121 Foggia, ItalyNational Research Council of Italy, Institute of Biomedical Technologies, Section of Bari, via Amendola no. 122/D, 70126 Bari, ItalyNational Research Council of Italy, Institute of Biomedical Technologies, Section of Bari, via Amendola no. 122/D, 70126 Bari, ItalyDepartment of Medical and Surgical Sciences, University of Foggia, 71121 Foggia, ItalyNational Research Council of Italy, Institute of Biomedical Technologies, Section of Bari, via Amendola no. 122/D, 70126 Bari, ItalyNational Research Council of Italy, Institute of Biomedical Technologies, Section of Bari, via Amendola no. 122/D, 70126 Bari, ItalyDepartment of Medical and Surgical Sciences, University of Foggia, 71121 Foggia, ItalyNational Research Council of Italy, Institute of Biomedical Technologies, Section of Bari, via Amendola no. 122/D, 70126 Bari, ItalyExtracellular vesicles (EVs), nanoparticles originated from different cell types, seem to be implicated in several cellular activities. In the Central Nervous System (CNS), glia and neurons secrete EVs and recent studies have demonstrated that the intercellular communication mediated by EVs has versatile functional impact in the cerebral homeostasis. This essential role may be due to their proteins and RNAs cargo that possibly modify the phenotypes of the targeted cells. Despite the increasing importance of EVs, little is known about their fluctuations in physiological as well as in pathological conditions. Furthermore, only few studies have investigated the contents of contemporary EVs subgroups (microvesicles, MVs and exosomes, EXOs) with the purpose of discriminating between their features and functional roles. In order to possibly shed light on these issues, we performed a pilot study in which MVs and EXOs extracted from serum samples of a little cohort of subjects (patients with the first clinical evidence of CNS demyelination, also known as Clinically Isolated Syndrome and Healthy Controls) were submitted to deep small-RNA sequencing. Data were analysed by an in-home bioinformatics platform. In line with previous reports, distinct classes of non-coding RNAs have been detected in both the EVs subsets, offering interesting suggestions on their origins and functions. We also verified the feasibility of this extensive molecular approach, thus supporting its valuable use for the analysis of circulating biomarkers (e.g., microRNAs) in order to investigate and monitor specific diseases.https://www.mdpi.com/2076-3271/5/3/19extracellular vesicles (EVs)Clinically Isolated Syndrome (CIS)small RNA sequencingcirculating biomarkersbioinformatics |
| spellingShingle | Nicoletta Nuzziello Maria Blonda Flavio Licciulli Sabino Liuni Antonella Amoruso Alessio Valletti Arianna Consiglio Carlo Avolio Maria Liguori Molecular Characterization of Peripheral Extracellular Vesicles in Clinically Isolated Syndrome: Preliminary Suggestions from a Pilot Study Medical Sciences extracellular vesicles (EVs) Clinically Isolated Syndrome (CIS) small RNA sequencing circulating biomarkers bioinformatics |
| title | Molecular Characterization of Peripheral Extracellular Vesicles in Clinically Isolated Syndrome: Preliminary Suggestions from a Pilot Study |
| title_full | Molecular Characterization of Peripheral Extracellular Vesicles in Clinically Isolated Syndrome: Preliminary Suggestions from a Pilot Study |
| title_fullStr | Molecular Characterization of Peripheral Extracellular Vesicles in Clinically Isolated Syndrome: Preliminary Suggestions from a Pilot Study |
| title_full_unstemmed | Molecular Characterization of Peripheral Extracellular Vesicles in Clinically Isolated Syndrome: Preliminary Suggestions from a Pilot Study |
| title_short | Molecular Characterization of Peripheral Extracellular Vesicles in Clinically Isolated Syndrome: Preliminary Suggestions from a Pilot Study |
| title_sort | molecular characterization of peripheral extracellular vesicles in clinically isolated syndrome preliminary suggestions from a pilot study |
| topic | extracellular vesicles (EVs) Clinically Isolated Syndrome (CIS) small RNA sequencing circulating biomarkers bioinformatics |
| url | https://www.mdpi.com/2076-3271/5/3/19 |
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