Antimicrobial effect of essential oil of Citrus reticulata on Fusobacterium nucleatum associated with periodontal disease
Chlorhexidine as a treatment of periodontal disease has achieved bactericidal effects over periodontopathogens and oral biofilm. Its use generates adverse effects; therefore natural alternatives are presented with a similar antimicrobial effect. Essential oils have proved effective in controlling de...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | Spanish |
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Universidad Nacional de Colombia
2017-07-01
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| Series: | Revista Colombiana de Biotecnología |
| Subjects: | |
| Online Access: | https://revistas.unal.edu.co/index.php/biotecnologia/article/view/57921 |
| _version_ | 1818999548616376320 |
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| author | Cindy Giohanna Pardo Gladys Stella Monsalve Herlinto Alveiro Tupaz Erira Yeison Espinosa Villamizar Gloria Isabel Jaramillo |
| author_facet | Cindy Giohanna Pardo Gladys Stella Monsalve Herlinto Alveiro Tupaz Erira Yeison Espinosa Villamizar Gloria Isabel Jaramillo |
| author_sort | Cindy Giohanna Pardo |
| collection | DOAJ |
| description | Chlorhexidine as a treatment of periodontal disease has achieved bactericidal effects over periodontopathogens and oral biofilm. Its use generates adverse effects; therefore natural alternatives are presented with a similar antimicrobial effect. Essential oils have proved effective in controlling dental plaque without the adverse effects of chlorhexidine. The aim of this study was to determine the bacteriostatic and bactericidal effect of essential oil of tangerine against Fusobacterium nucleatum. The extraction of the essential oil was performed by expression of tangerine peels (Arrayana and Oneco varieties). Concentrations at 20%, 40%, 60%, 80% and 100% of the essential oil diluted in 0,02% Tween were evaluated. The bacteriostatic and bactericidal effect was determined by antimicrobial susceptibility testing by disk diffusion. As a positive control 0,2% chlorhexidine and water as negative control were used. Inhibition zone (mm) was measured and presence or absence of bacterial growth was determined from colony forming units. To compare proportions of bacteriostatic and bactericidal activity, Fisher and T student test (95% CI p = 0,05) were performed. The 100% concentration zone of inhibition showed a similar behavior as chlorhexidine (p <0,05). 100% and 80% concentrations were bactericides, 60%, 40% and 20% showed bacteriostatic behavior. No significant differences between the proportions of inhibition of the two varieties of tangerine (p> 0,05). The use of essential oils of tangerine could be a complementary alternative to treatment of periodontal disease. |
| first_indexed | 2024-12-20T22:19:11Z |
| format | Article |
| id | doaj.art-725aac16335c459cbdf2ed0a58f2c883 |
| institution | Directory Open Access Journal |
| issn | 0123-3475 1909-8758 |
| language | Spanish |
| last_indexed | 2024-12-20T22:19:11Z |
| publishDate | 2017-07-01 |
| publisher | Universidad Nacional de Colombia |
| record_format | Article |
| series | Revista Colombiana de Biotecnología |
| spelling | doaj.art-725aac16335c459cbdf2ed0a58f2c8832022-12-21T19:24:58ZspaUniversidad Nacional de ColombiaRevista Colombiana de Biotecnología0123-34751909-87582017-07-0119271410.15446/rev.colomb.biote.v19n2.5792147205Antimicrobial effect of essential oil of Citrus reticulata on Fusobacterium nucleatum associated with periodontal diseaseCindy Giohanna Pardo0Gladys Stella Monsalve1Herlinto Alveiro Tupaz Erira2Yeison Espinosa Villamizar3Gloria Isabel Jaramillo4Universidad Cooperativa de ColombiaUniversidad Cooperativa de ColombiaUniversidad Cooperativa de ColombiaUniversidad Cooperativa de ColombiaUniversidad Cooperativa de ColombiaChlorhexidine as a treatment of periodontal disease has achieved bactericidal effects over periodontopathogens and oral biofilm. Its use generates adverse effects; therefore natural alternatives are presented with a similar antimicrobial effect. Essential oils have proved effective in controlling dental plaque without the adverse effects of chlorhexidine. The aim of this study was to determine the bacteriostatic and bactericidal effect of essential oil of tangerine against Fusobacterium nucleatum. The extraction of the essential oil was performed by expression of tangerine peels (Arrayana and Oneco varieties). Concentrations at 20%, 40%, 60%, 80% and 100% of the essential oil diluted in 0,02% Tween were evaluated. The bacteriostatic and bactericidal effect was determined by antimicrobial susceptibility testing by disk diffusion. As a positive control 0,2% chlorhexidine and water as negative control were used. Inhibition zone (mm) was measured and presence or absence of bacterial growth was determined from colony forming units. To compare proportions of bacteriostatic and bactericidal activity, Fisher and T student test (95% CI p = 0,05) were performed. The 100% concentration zone of inhibition showed a similar behavior as chlorhexidine (p <0,05). 100% and 80% concentrations were bactericides, 60%, 40% and 20% showed bacteriostatic behavior. No significant differences between the proportions of inhibition of the two varieties of tangerine (p> 0,05). The use of essential oils of tangerine could be a complementary alternative to treatment of periodontal disease.https://revistas.unal.edu.co/index.php/biotecnologia/article/view/57921Aceite esencialperiodontopatógenosCitricos |
| spellingShingle | Cindy Giohanna Pardo Gladys Stella Monsalve Herlinto Alveiro Tupaz Erira Yeison Espinosa Villamizar Gloria Isabel Jaramillo Antimicrobial effect of essential oil of Citrus reticulata on Fusobacterium nucleatum associated with periodontal disease Revista Colombiana de Biotecnología Aceite esencial periodontopatógenos Citricos |
| title | Antimicrobial effect of essential oil of Citrus reticulata on Fusobacterium nucleatum associated with periodontal disease |
| title_full | Antimicrobial effect of essential oil of Citrus reticulata on Fusobacterium nucleatum associated with periodontal disease |
| title_fullStr | Antimicrobial effect of essential oil of Citrus reticulata on Fusobacterium nucleatum associated with periodontal disease |
| title_full_unstemmed | Antimicrobial effect of essential oil of Citrus reticulata on Fusobacterium nucleatum associated with periodontal disease |
| title_short | Antimicrobial effect of essential oil of Citrus reticulata on Fusobacterium nucleatum associated with periodontal disease |
| title_sort | antimicrobial effect of essential oil of citrus reticulata on fusobacterium nucleatum associated with periodontal disease |
| topic | Aceite esencial periodontopatógenos Citricos |
| url | https://revistas.unal.edu.co/index.php/biotecnologia/article/view/57921 |
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