Diversity of bioprotective microbial organisms in Upper Region of Assam and its efficacy against Meloidogyne graminicola
Meloidogyne graminicola has a well-established negative impact on rice yield in transplanted and direct-seeded rice, resulting in yield losses of up to 20 to 90 percent. Studies were undertaken to isolate potential native strains of bio-control agents to manage the devastating Rice Root Knot Nematod...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
PeerJ Inc.
2023-07-01
|
| Series: | PeerJ |
| Subjects: | |
| Online Access: | https://peerj.com/articles/15779.pdf |
| _version_ | 1797420935316242432 |
|---|---|
| author | Rupak Jena Bhupendranath Choudhury Debanand Das Bhabesh Bhagawati Pradip Kumar Borah Seenichamy Rathinam Prabhukartikeyan Swoyam Singh Manaswini Mahapatra Milan Kumar Lal Rahul Kumar Tiwari Ravinder Kumar |
| author_facet | Rupak Jena Bhupendranath Choudhury Debanand Das Bhabesh Bhagawati Pradip Kumar Borah Seenichamy Rathinam Prabhukartikeyan Swoyam Singh Manaswini Mahapatra Milan Kumar Lal Rahul Kumar Tiwari Ravinder Kumar |
| author_sort | Rupak Jena |
| collection | DOAJ |
| description | Meloidogyne graminicola has a well-established negative impact on rice yield in transplanted and direct-seeded rice, resulting in yield losses of up to 20 to 90 percent. Studies were undertaken to isolate potential native strains of bio-control agents to manage the devastating Rice Root Knot Nematode (M. graminicola). Eighteen bacterial strains and eleven fungal strains were isolated from the rhizosphere of crops like rice, okra, ash gourd, chili, beans and cucumber, enveloping diverse soil types from the Upper Brahmaputra Valley region of Assam. Six bacterial strains were gram-positive according to morphological results, while twelve others stained negatively. Fifteen bacteria were rod-shaped, two were coccus and one was diplococcus, and all the bacterial isolates showed signs of movement. All the bacterial strains exhibited positivity for gelatin hydrolysis and catalase test. Seven bacteria showed positive, while eleven showed negative reactions to possess the ability to deduce carbon and energy from citrate. The study of the in vitro efficacy of the twenty-nine bacterial and fungal isolates tested against second-stage juveniles (J2) of Meloidogyne graminicola revealed that all the bacterial and fungal isolates potentially inhibited the test organism and caused significant mortality over sterile water treatment. The promising bacterial and fungal isolates that exhibited mortality above 50% were identified as BSH8, BTS4, BTS5, BJA15, FJB 11 and FSH5. The strain BSH8 exhibited the best result of mortality, with 80.79% mortality against J2 of M. graminicola. The strain BTS4 and BTS5 expressed mortality of 71.29% and 68.75% under in-vitro conditions and were significant. The effective and promising bioagents were identified using the 16 S rRNA sequencing as Bacillis subtilis (BSH8), Bacillus velezensis (BTS4), Alcaligenes faecalis (BTS5), Rhizobium pusense (BJA15), Talaromyces allahabadensis (FSH5) and Trichoderma asperellum (FJB11). These results indicated the microorganism’s potential against M. graminicola and its potential for successful biological implementation. Further, the native strains could be tested against various nematode pests of rice in field conditions. Its compatibility with various pesticides and the implication of the potential strains in integrated pest management can be assessed. |
| first_indexed | 2024-03-09T07:09:25Z |
| format | Article |
| id | doaj.art-725bdbe3e4524755b270fac7832861f1 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T07:09:25Z |
| publishDate | 2023-07-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-725bdbe3e4524755b270fac7832861f12023-12-03T09:16:03ZengPeerJ Inc.PeerJ2167-83592023-07-0111e1577910.7717/peerj.15779Diversity of bioprotective microbial organisms in Upper Region of Assam and its efficacy against Meloidogyne graminicolaRupak Jena0Bhupendranath Choudhury1Debanand Das2Bhabesh Bhagawati3Pradip Kumar Borah4Seenichamy Rathinam Prabhukartikeyan5Swoyam Singh6Manaswini Mahapatra7Milan Kumar Lal8Rahul Kumar Tiwari9Ravinder Kumar10Department of Nematology, Assam Agricultural University, Jorhat, Assam, IndiaDepartment of Nematology, Assam Agricultural University, Jorhat, Assam, IndiaDepartment of Nematology, Assam Agricultural University, Jorhat, Assam, IndiaDepartment of Nematology, Assam Agricultural University, Jorhat, Assam, IndiaDepartment of Nematology, Assam Agricultural University, Jorhat, Assam, IndiaDivision of Crop Protection, National Rice Research Institute, Cuttack, Odisha, IndiaDepartment of Entomology, Siksha O Anusandhan, Bhubaneswar, Odisha, IndiaDepartment of Agriculture and Allied Sciences (Plant Pathology), C.V. Raman Global University, Bhubaneswar, Odisha, IndiaDivision of Plant Protection, ICAR-Central Potato Research Institute, Shimla, Himachal Pradesh, IndiaDivision of Plant Protection, ICAR-Central Potato Research Institute, Shimla, Himachal Pradesh, IndiaDivision of Plant Protection, ICAR-Central Potato Research Institute, Shimla, Himachal Pradesh, IndiaMeloidogyne graminicola has a well-established negative impact on rice yield in transplanted and direct-seeded rice, resulting in yield losses of up to 20 to 90 percent. Studies were undertaken to isolate potential native strains of bio-control agents to manage the devastating Rice Root Knot Nematode (M. graminicola). Eighteen bacterial strains and eleven fungal strains were isolated from the rhizosphere of crops like rice, okra, ash gourd, chili, beans and cucumber, enveloping diverse soil types from the Upper Brahmaputra Valley region of Assam. Six bacterial strains were gram-positive according to morphological results, while twelve others stained negatively. Fifteen bacteria were rod-shaped, two were coccus and one was diplococcus, and all the bacterial isolates showed signs of movement. All the bacterial strains exhibited positivity for gelatin hydrolysis and catalase test. Seven bacteria showed positive, while eleven showed negative reactions to possess the ability to deduce carbon and energy from citrate. The study of the in vitro efficacy of the twenty-nine bacterial and fungal isolates tested against second-stage juveniles (J2) of Meloidogyne graminicola revealed that all the bacterial and fungal isolates potentially inhibited the test organism and caused significant mortality over sterile water treatment. The promising bacterial and fungal isolates that exhibited mortality above 50% were identified as BSH8, BTS4, BTS5, BJA15, FJB 11 and FSH5. The strain BSH8 exhibited the best result of mortality, with 80.79% mortality against J2 of M. graminicola. The strain BTS4 and BTS5 expressed mortality of 71.29% and 68.75% under in-vitro conditions and were significant. The effective and promising bioagents were identified using the 16 S rRNA sequencing as Bacillis subtilis (BSH8), Bacillus velezensis (BTS4), Alcaligenes faecalis (BTS5), Rhizobium pusense (BJA15), Talaromyces allahabadensis (FSH5) and Trichoderma asperellum (FJB11). These results indicated the microorganism’s potential against M. graminicola and its potential for successful biological implementation. Further, the native strains could be tested against various nematode pests of rice in field conditions. Its compatibility with various pesticides and the implication of the potential strains in integrated pest management can be assessed.https://peerj.com/articles/15779.pdfRhizosphericMeloidogyne graminicolaMortalityMicrobiologyBacillus velezensis |
| spellingShingle | Rupak Jena Bhupendranath Choudhury Debanand Das Bhabesh Bhagawati Pradip Kumar Borah Seenichamy Rathinam Prabhukartikeyan Swoyam Singh Manaswini Mahapatra Milan Kumar Lal Rahul Kumar Tiwari Ravinder Kumar Diversity of bioprotective microbial organisms in Upper Region of Assam and its efficacy against Meloidogyne graminicola PeerJ Rhizospheric Meloidogyne graminicola Mortality Microbiology Bacillus velezensis |
| title | Diversity of bioprotective microbial organisms in Upper Region of Assam and its efficacy against Meloidogyne graminicola |
| title_full | Diversity of bioprotective microbial organisms in Upper Region of Assam and its efficacy against Meloidogyne graminicola |
| title_fullStr | Diversity of bioprotective microbial organisms in Upper Region of Assam and its efficacy against Meloidogyne graminicola |
| title_full_unstemmed | Diversity of bioprotective microbial organisms in Upper Region of Assam and its efficacy against Meloidogyne graminicola |
| title_short | Diversity of bioprotective microbial organisms in Upper Region of Assam and its efficacy against Meloidogyne graminicola |
| title_sort | diversity of bioprotective microbial organisms in upper region of assam and its efficacy against meloidogyne graminicola |
| topic | Rhizospheric Meloidogyne graminicola Mortality Microbiology Bacillus velezensis |
| url | https://peerj.com/articles/15779.pdf |
| work_keys_str_mv | AT rupakjena diversityofbioprotectivemicrobialorganismsinupperregionofassamanditsefficacyagainstmeloidogynegraminicola AT bhupendranathchoudhury diversityofbioprotectivemicrobialorganismsinupperregionofassamanditsefficacyagainstmeloidogynegraminicola AT debananddas diversityofbioprotectivemicrobialorganismsinupperregionofassamanditsefficacyagainstmeloidogynegraminicola AT bhabeshbhagawati diversityofbioprotectivemicrobialorganismsinupperregionofassamanditsefficacyagainstmeloidogynegraminicola AT pradipkumarborah diversityofbioprotectivemicrobialorganismsinupperregionofassamanditsefficacyagainstmeloidogynegraminicola AT seenichamyrathinamprabhukartikeyan diversityofbioprotectivemicrobialorganismsinupperregionofassamanditsefficacyagainstmeloidogynegraminicola AT swoyamsingh diversityofbioprotectivemicrobialorganismsinupperregionofassamanditsefficacyagainstmeloidogynegraminicola AT manaswinimahapatra diversityofbioprotectivemicrobialorganismsinupperregionofassamanditsefficacyagainstmeloidogynegraminicola AT milankumarlal diversityofbioprotectivemicrobialorganismsinupperregionofassamanditsefficacyagainstmeloidogynegraminicola AT rahulkumartiwari diversityofbioprotectivemicrobialorganismsinupperregionofassamanditsefficacyagainstmeloidogynegraminicola AT ravinderkumar diversityofbioprotectivemicrobialorganismsinupperregionofassamanditsefficacyagainstmeloidogynegraminicola |