Diversity of antimicrobial-resistant bacteria isolated from Australian chicken and pork meat
Antimicrobial-resistant bacteria are frequently isolated from retail meat and may infect humans. To determine the diversity of antimicrobial-resistant bacteria in Australian retail meat, bacteria were cultured on selective media from raw chicken (n = 244) and pork (n = 160) meat samples obtained fro...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-02-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1347597/full |
| _version_ | 1797303321904545792 |
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| author | Ojas V. A. Dixit Ojas V. A. Dixit Mahboobeh Behruznia Aidan L. Preuss Claire L. O’Brien |
| author_facet | Ojas V. A. Dixit Ojas V. A. Dixit Mahboobeh Behruznia Aidan L. Preuss Claire L. O’Brien |
| author_sort | Ojas V. A. Dixit |
| collection | DOAJ |
| description | Antimicrobial-resistant bacteria are frequently isolated from retail meat and may infect humans. To determine the diversity of antimicrobial-resistant bacteria in Australian retail meat, bacteria were cultured on selective media from raw chicken (n = 244) and pork (n = 160) meat samples obtained from all four major supermarket chains in the ACT/NSW, Australia, between March and June 2021. Antimicrobial susceptibility testing (AST) was performed for 13 critically and 4 highly important antibiotics as categorised by the World Health Organization (WHO) for a wide range of species detected in the meat samples. A total of 288 isolates underwent whole-genome sequencing (WGS) to identify the presence of antimicrobial resistance (AMR) genes, virulence genes, and plasmids. AST testing revealed that 35/288 (12%) of the isolates were found to be multidrug-resistant (MDR). Using WGS data, 232/288 (81%) of the isolates were found to harbour resistance genes for critically or highly important antibiotics. This study reveals a greater diversity of AMR genes in bacteria isolated from retail meat in Australia than previous studies have shown, emphasising the importance of monitoring AMR in not only foodborne pathogenic bacteria, but other species that are capable of transferring AMR genes to pathogenic bacteria. |
| first_indexed | 2024-03-07T23:51:07Z |
| format | Article |
| id | doaj.art-725bdf5a79964c27b14c3f18f232622e |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-07T23:51:07Z |
| publishDate | 2024-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-725bdf5a79964c27b14c3f18f232622e2024-02-19T04:56:47ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2024-02-011510.3389/fmicb.2024.13475971347597Diversity of antimicrobial-resistant bacteria isolated from Australian chicken and pork meatOjas V. A. Dixit0Ojas V. A. Dixit1Mahboobeh Behruznia2Aidan L. Preuss3Claire L. O’Brien4Faculty of Science and Technology, University of Canberra, Canberra, ACT, AustraliaSchool of Medicine, Science, Medicine and Health, University of Wollongong, Wollongong, NSW, AustraliaFaculty of Science and Technology, University of Canberra, Canberra, ACT, AustraliaFaculty of Science and Technology, University of Canberra, Canberra, ACT, AustraliaFaculty of Science and Technology, University of Canberra, Canberra, ACT, AustraliaAntimicrobial-resistant bacteria are frequently isolated from retail meat and may infect humans. To determine the diversity of antimicrobial-resistant bacteria in Australian retail meat, bacteria were cultured on selective media from raw chicken (n = 244) and pork (n = 160) meat samples obtained from all four major supermarket chains in the ACT/NSW, Australia, between March and June 2021. Antimicrobial susceptibility testing (AST) was performed for 13 critically and 4 highly important antibiotics as categorised by the World Health Organization (WHO) for a wide range of species detected in the meat samples. A total of 288 isolates underwent whole-genome sequencing (WGS) to identify the presence of antimicrobial resistance (AMR) genes, virulence genes, and plasmids. AST testing revealed that 35/288 (12%) of the isolates were found to be multidrug-resistant (MDR). Using WGS data, 232/288 (81%) of the isolates were found to harbour resistance genes for critically or highly important antibiotics. This study reveals a greater diversity of AMR genes in bacteria isolated from retail meat in Australia than previous studies have shown, emphasising the importance of monitoring AMR in not only foodborne pathogenic bacteria, but other species that are capable of transferring AMR genes to pathogenic bacteria.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1347597/fullantibioticantibiotic resistanceantimicrobial resistanceantimicrobial susceptibility testingmultidrug-resistanceresistance genes |
| spellingShingle | Ojas V. A. Dixit Ojas V. A. Dixit Mahboobeh Behruznia Aidan L. Preuss Claire L. O’Brien Diversity of antimicrobial-resistant bacteria isolated from Australian chicken and pork meat Frontiers in Microbiology antibiotic antibiotic resistance antimicrobial resistance antimicrobial susceptibility testing multidrug-resistance resistance genes |
| title | Diversity of antimicrobial-resistant bacteria isolated from Australian chicken and pork meat |
| title_full | Diversity of antimicrobial-resistant bacteria isolated from Australian chicken and pork meat |
| title_fullStr | Diversity of antimicrobial-resistant bacteria isolated from Australian chicken and pork meat |
| title_full_unstemmed | Diversity of antimicrobial-resistant bacteria isolated from Australian chicken and pork meat |
| title_short | Diversity of antimicrobial-resistant bacteria isolated from Australian chicken and pork meat |
| title_sort | diversity of antimicrobial resistant bacteria isolated from australian chicken and pork meat |
| topic | antibiotic antibiotic resistance antimicrobial resistance antimicrobial susceptibility testing multidrug-resistance resistance genes |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1347597/full |
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