Comparison of Two Commercial ELISA Kits for the Detection of Anti-Dengue IgM for Routine Dengue Diagnosis in Laos
The endemicity of Dengue virus (DENV) infection remains a major public health problem in Lao PDR. In this study, we compared two commercial anti-dengue IgM ELISA kits, Panbio<sup>®</sup> Dengue IgM Capture ELISA (Panbio Kit, Alere, Waltham, MA, USA) and DEN Detect<sup>TM&l...
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| Format: | Article |
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MDPI AG
2019-07-01
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| Series: | Tropical Medicine and Infectious Disease |
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| Online Access: | https://www.mdpi.com/2414-6366/4/3/111 |
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| author | Yixiao Lu Onanong Sengvilaipaseuth Anisone Chanthongthip Ooyanong Phonemixay Manivanh Vongsouvath Phonelavanh Phouminh Stuart D. Blacksell Paul N. Newton Audrey Dubot-Pérès |
| author_facet | Yixiao Lu Onanong Sengvilaipaseuth Anisone Chanthongthip Ooyanong Phonemixay Manivanh Vongsouvath Phonelavanh Phouminh Stuart D. Blacksell Paul N. Newton Audrey Dubot-Pérès |
| author_sort | Yixiao Lu |
| collection | DOAJ |
| description | The endemicity of Dengue virus (DENV) infection remains a major public health problem in Lao PDR. In this study, we compared two commercial anti-dengue IgM ELISA kits, Panbio<sup>®</sup> Dengue IgM Capture ELISA (Panbio Kit, Alere, Waltham, MA, USA) and DEN Detect<sup>TM</sup> MAC-ELISA (InBios kit, InBios International, Inc., Seattle, WA, USA), in the context of diagnosis of patients admitted to hospital with clinical dengue presentation. Two panels of paired blood samples were tested. Panel A was composed of 54 dengue confirmed patients (by DENV real-time RT-PCR) and 11 non-dengue dengue patients (other infections confirmed by corresponding PCR results). Panel B included 74 patients randomly selected from consecutive patients admitted to Mahosot Hospital in 2008 with suspicion of dengue fever according to WHO criteria. Results from panel A showed significantly better sensitivity for Panbio kit (64.8%; 95%CI: 50.6−77.3%) than for InBios kit (18.5%; 95%CI: 9.3−31.4%) when testing admission sera. Sensitivity was increased for both kits when combining results from admission and convalescent sera. Concordant results were obtained from panel B with fair agreement (κ = 0.29) between both kits when testing single admission samples, and moderate agreement (κ = 0.5) when combining results from admission and convalescent sera. |
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| issn | 2414-6366 |
| language | English |
| last_indexed | 2024-04-12T19:35:19Z |
| publishDate | 2019-07-01 |
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| series | Tropical Medicine and Infectious Disease |
| spelling | doaj.art-725be468b75949ce8034cedd96595d162022-12-22T03:19:14ZengMDPI AGTropical Medicine and Infectious Disease2414-63662019-07-014311110.3390/tropicalmed4030111tropicalmed4030111Comparison of Two Commercial ELISA Kits for the Detection of Anti-Dengue IgM for Routine Dengue Diagnosis in LaosYixiao Lu0Onanong Sengvilaipaseuth1Anisone Chanthongthip2Ooyanong Phonemixay3Manivanh Vongsouvath4Phonelavanh Phouminh5Stuart D. Blacksell6Paul N. Newton7Audrey Dubot-Pérès8Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit (LOMWRU), Microbiology Laboratory, Mahosot Hospital, Vientiane 01000, LaosLao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit (LOMWRU), Microbiology Laboratory, Mahosot Hospital, Vientiane 01000, LaosLao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit (LOMWRU), Microbiology Laboratory, Mahosot Hospital, Vientiane 01000, LaosLao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit (LOMWRU), Microbiology Laboratory, Mahosot Hospital, Vientiane 01000, LaosLao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit (LOMWRU), Microbiology Laboratory, Mahosot Hospital, Vientiane 01000, LaosLao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit (LOMWRU), Microbiology Laboratory, Mahosot Hospital, Vientiane 01000, LaosMahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, ThailandLao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit (LOMWRU), Microbiology Laboratory, Mahosot Hospital, Vientiane 01000, LaosLao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit (LOMWRU), Microbiology Laboratory, Mahosot Hospital, Vientiane 01000, LaosThe endemicity of Dengue virus (DENV) infection remains a major public health problem in Lao PDR. In this study, we compared two commercial anti-dengue IgM ELISA kits, Panbio<sup>®</sup> Dengue IgM Capture ELISA (Panbio Kit, Alere, Waltham, MA, USA) and DEN Detect<sup>TM</sup> MAC-ELISA (InBios kit, InBios International, Inc., Seattle, WA, USA), in the context of diagnosis of patients admitted to hospital with clinical dengue presentation. Two panels of paired blood samples were tested. Panel A was composed of 54 dengue confirmed patients (by DENV real-time RT-PCR) and 11 non-dengue dengue patients (other infections confirmed by corresponding PCR results). Panel B included 74 patients randomly selected from consecutive patients admitted to Mahosot Hospital in 2008 with suspicion of dengue fever according to WHO criteria. Results from panel A showed significantly better sensitivity for Panbio kit (64.8%; 95%CI: 50.6−77.3%) than for InBios kit (18.5%; 95%CI: 9.3−31.4%) when testing admission sera. Sensitivity was increased for both kits when combining results from admission and convalescent sera. Concordant results were obtained from panel B with fair agreement (κ = 0.29) between both kits when testing single admission samples, and moderate agreement (κ = 0.5) when combining results from admission and convalescent sera.https://www.mdpi.com/2414-6366/4/3/111dengueMAC-ELISAserologyLaosIgM |
| spellingShingle | Yixiao Lu Onanong Sengvilaipaseuth Anisone Chanthongthip Ooyanong Phonemixay Manivanh Vongsouvath Phonelavanh Phouminh Stuart D. Blacksell Paul N. Newton Audrey Dubot-Pérès Comparison of Two Commercial ELISA Kits for the Detection of Anti-Dengue IgM for Routine Dengue Diagnosis in Laos Tropical Medicine and Infectious Disease dengue MAC-ELISA serology Laos IgM |
| title | Comparison of Two Commercial ELISA Kits for the Detection of Anti-Dengue IgM for Routine Dengue Diagnosis in Laos |
| title_full | Comparison of Two Commercial ELISA Kits for the Detection of Anti-Dengue IgM for Routine Dengue Diagnosis in Laos |
| title_fullStr | Comparison of Two Commercial ELISA Kits for the Detection of Anti-Dengue IgM for Routine Dengue Diagnosis in Laos |
| title_full_unstemmed | Comparison of Two Commercial ELISA Kits for the Detection of Anti-Dengue IgM for Routine Dengue Diagnosis in Laos |
| title_short | Comparison of Two Commercial ELISA Kits for the Detection of Anti-Dengue IgM for Routine Dengue Diagnosis in Laos |
| title_sort | comparison of two commercial elisa kits for the detection of anti dengue igm for routine dengue diagnosis in laos |
| topic | dengue MAC-ELISA serology Laos IgM |
| url | https://www.mdpi.com/2414-6366/4/3/111 |
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