Genetic Knock-Ins of Endogenous Fluorescent Tags in RAW 264.7 Murine Macrophages Using CRISPR/Cas9 Genome Editing

Genetic Knock-Ins of Endogenous Fluorescent Tags in RAW 264.7 Murine Macrophages Using CRISPR/Cas9 Genome Editing

CRISPR/Cas9 genome editing is a widely used tool for creating genetic knock-ins, which allow for endogenous tagging of genes. This is in contrast with random insertion using viral vectors, where expression of the inserted transgene changes the total copy number of a gene in a cell and does not refle...

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Dettagli Bibliografici
Autori principali:	Beverly Naigles, Jan Soroczynski, Nan Hao
Natura:	Articolo
Lingua:	English
Pubblicazione:	Bio-protocol LLC 2024-03-01
Serie:	Bio-Protocol
Accesso online:	https://bio-protocol.org/en/bpdetail?id=4960&type=0

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