Genetic Knock-Ins of Endogenous Fluorescent Tags in RAW 264.7 Murine Macrophages Using CRISPR/Cas9 Genome Editing

Genetic Knock-Ins of Endogenous Fluorescent Tags in RAW 264.7 Murine Macrophages Using CRISPR/Cas9 Genome Editing

CRISPR/Cas9 genome editing is a widely used tool for creating genetic knock-ins, which allow for endogenous tagging of genes. This is in contrast with random insertion using viral vectors, where expression of the inserted transgene changes the total copy number of a gene in a cell and does not refle...

ver descrição completa

Detalhes bibliográficos
Main Authors: Beverly Naigles, Jan Soroczynski, Nan Hao
Formato: Artigo
Idioma:English
Publicado em: Bio-protocol LLC 2024-03-01
Colecção:Bio-Protocol
Acesso em linha:https://bio-protocol.org/en/bpdetail?id=4960&type=0

Registos relacionados

Registos relacionados