Genetic Knock-Ins of Endogenous Fluorescent Tags in RAW 264.7 Murine Macrophages Using CRISPR/Cas9 Genome Editing

Genetic Knock-Ins of Endogenous Fluorescent Tags in RAW 264.7 Murine Macrophages Using CRISPR/Cas9 Genome Editing

CRISPR/Cas9 genome editing is a widely used tool for creating genetic knock-ins, which allow for endogenous tagging of genes. This is in contrast with random insertion using viral vectors, where expression of the inserted transgene changes the total copy number of a gene in a cell and does not refle...

书目详细资料
Main Authors:	Beverly Naigles, Jan Soroczynski, Nan Hao
格式:	文件
语言:	English
出版:	Bio-protocol LLC 2024-03-01
丛编:	Bio-Protocol
在线阅读:	https://bio-protocol.org/en/bpdetail?id=4960&type=0

相似书籍

Creating a RAW264.7 CRISPR-Cas9 Genome Wide Library
由: Brooke Napier, et al.
出版: (2017-05-01)

Generation of epitope tag knock-in mice with CRISPR-Cas9 to study the function of endogenous proteins
由: Zhao Zhang
出版: (2023-09-01)

Endogenous protein tagging in medaka using a simplified CRISPR/Cas9 knock-in approach
由: Ali Seleit, et al.
出版: (2021-12-01)

Viral Population Changes during Murine Norovirus Propagation in RAW 264.7 Cells
由: Takuya Kitamoto, et al.
出版: (2017-06-01)

The uptake of tocopherols by RAW 264.7 macrophages
由: Papas Andreas M, et al.
出版: (2002-10-01)

Generation and characterization of an endogenously tagged SPG11-human iPSC line by CRISPR/Cas9 mediated knock-in
由: Laura Krumm, et al.
出版: (2021-10-01)

Protocol for HiBiT tagging endogenous proteins using CRISPR-Cas9 gene editing
由: Kaylee P. Lankford, et al.
出版: (2024-06-01)

Evaluation of Methods for Transient Transfection of a Murine Macrophage Cell Line, RAW 264.7
由: C.D. Thompson, et al.
出版: (1999-10-01)

Effect of Porphyridium cruentum polysaccharides on the activity of murine macrophage cell line RAW 264.7
由: R T Abdala-Diaz, et al.
出版: (2010-08-01)

Osteoclasts Differentiation from Murine RAW 264.7 Cells Stimulated by RANKL: Timing and Behavior
由: Nadia Lampiasi, et al.
出版: (2021-02-01)

Anti-Inflammatory Activity of Bryophytes Extracts in LPS-Stimulated RAW264.7 Murine Macrophages
由: Raíssa Volpatto Marques, et al.
出版: (2022-03-01)

Jeju seaweeds suppress lipopolysaccharide-stimulated proinflammatory response in RAW 264.7 murine macrophages
由: Eun-Jin Yang, et al.
出版: (2014-07-01)

Anti-inflammatory activities of Italian Chestnut and Eucalyptus honeys on murine RAW 264.7 macrophages
由: Danila Cianciosi, et al.
出版: (2021-12-01)

Mycobacterial nucleoside diphosphate kinase blocks phagosome maturation in murine RAW 264.7 macrophages.
由: Jim Sun, et al.
出版: (2010-01-01)

Effect of pulsed electromagnetic field on inflammatory pathway markers in RAW 264.7 murine macrophages
由: Ross CL, et al.
出版: (2013-03-01)

Proteomic analysis of murine norovirus-infected Raw 264.7 cells reveals the induction of Ras GTPases
由: Sung Ho Yun, et al.
出版: (2025-01-01)

Rapamycin Augments the NMDA-Mediated TNF Suppression of MRSA-Stimulated RAW264.7 Murine Macrophages
由: Thomas Spentzas, et al.
出版: (2012-01-01)

Jatrorrhizine Suppresses Murine-Norovirus-Triggered N-GSDMD-Dependent Pyroptosis in RAW264.7 Macrophages
由: Ming Fu, et al.
出版: (2023-01-01)

Anti-inflammatory activities of Guang-Pheretima extract in lipopolysaccharide-stimulated RAW 264.7 murine macrophages
由: Chuanqi Huang, et al.
出版: (2018-02-01)

Stimulatory effects of polysaccharide fraction from Solanum nigrum on RAW 264.7 murine macrophage cells.
由: Faizan Naeem Razali, et al.
出版: (2014-01-01)

Sensing Peroxynitrite in Different Organelles of Murine RAW264.7 Macrophages With Coumarin-Based Fluorescent Probes
由: Maria Weber, et al.
出版: (2020-02-01)

Efficient generation of Knock-in/Knock-out marmoset embryo via CRISPR/Cas9 gene editing
由: Kumita, Wakako, et al.
出版: (2020)

Immunostimulating activity of Uncaria tomentosa in RAW 264.7 macrophages
由: A. S. D. Brito, et al.
出版: (2023-09-01)

Evaluation of culture conditions for osteoclastogenesis in RAW264.7 cells.
由: Yin Cheng, et al.
出版: (2022-01-01)

Sesamin Enhances Cholesterol Efflux in RAW264.7 Macrophages
由: Nan Liu, et al.
出版: (2014-06-01)

Immunostimulatory Effect of Laminarin on RAW 264.7 Mouse Macrophages
由: Wansu Park, et al.
出版: (2012-05-01)

ORANGE: A CRISPR/Cas9-based genome editing toolbox for epitope tagging of endogenous proteins in neurons.
由: Jelmer Willems, et al.
出版: (2020-04-01)

Blue Whiting Protein Hydrolysates Exhibit Antioxidant and Immunomodulatory Activities in Stimulated Murine RAW264.7 Cells
由: Shauna Heffernan, et al.
出版: (2021-10-01)

Anti-inflammatory effects of Torin2 on lipopolysaccharide-treated RAW264.7 murine macrophages and potential mechanisms
由: Shanshan Wei, et al.
出版: (2022-07-01)

Phenolic mediated anti-inflammatory properties of a maple syrup extract in RAW 264.7 murine macrophages
由: Pragati P. Nahar, et al.
出版: (2014-01-01)

Regulation of Inflammatory Cytokines in Lipopolysaccharide-Stimulated RAW 264.7 Murine Macrophage by 7-O-Methyl-naringenin
由: Jing Lu, et al.
出版: (2012-03-01)

Tagging to endogenous genes of Plasmodium falciparum using CRISPR/Cas9
由: Dexuan Kuang, et al.
出版: (2017-12-01)

Endogenous C-terminal Tagging by CRISPR/Cas9 in Trypanosoma cruzi
由: Noelia Lander, et al.
出版: (2017-05-01)

Taoren Honghua Drug Attenuates Atherosclerosis and Plays an Anti-Inflammatory Role in ApoE Knock-Out Mice and RAW264.7 Cells
由: Yiru Wang, et al.
出版: (2020-07-01)

Effect of phenelzine and serotonin on RAW264.7 macrophage cell viability
由: Saeed Mahdianipur, et al.
出版: (2024-10-01)

Effect of Inotodiol on LPS-induced Injury of RAW264.7 Cells
由: Yanbin ZHU, et al.
出版: (2023-10-01)

pH‐Responsive Motors and their Interaction with RAW 264.7 Macrophages
由: Miguel A. Ramos Docampo, et al.
出版: (2023-01-01)

Anti-inflammatory effect of chitosan oligosaccharides in RAW 264.7 cells
由: Yang Eun-Jin, et al.
出版: (2010-02-01)

Polyethylene terephthalate nanoparticles effect on RAW 264.7 macrophage cells
由: J. Cristóbal Aguilar-Guzmán, et al.
出版: (2022-02-01)

Optimized Staining Methods of Intracellular Calcium for RAW264.7 Cells
由: Zhang Jingzhi, et al.
出版: (2024-01-01)