Genetic Knock-Ins of Endogenous Fluorescent Tags in RAW 264.7 Murine Macrophages Using CRISPR/Cas9 Genome Editing

CRISPR/Cas9 genome editing is a widely used tool for creating genetic knock-ins, which allow for endogenous tagging of genes. This is in contrast with random insertion using viral vectors, where expression of the inserted transgene changes the total copy number of a gene in a cell and does not refle...

Disgrifiad llawn

Manylion Llyfryddiaeth
Prif Awduron: Beverly Naigles, Jan Soroczynski, Nan Hao
Fformat: Erthygl
Iaith:English
Cyhoeddwyd: Bio-protocol LLC 2024-03-01
Cyfres:Bio-Protocol
Mynediad Ar-lein:https://bio-protocol.org/en/bpdetail?id=4960&type=0