Genetic Knock-Ins of Endogenous Fluorescent Tags in RAW 264.7 Murine Macrophages Using CRISPR/Cas9 Genome Editing
CRISPR/Cas9 genome editing is a widely used tool for creating genetic knock-ins, which allow for endogenous tagging of genes. This is in contrast with random insertion using viral vectors, where expression of the inserted transgene changes the total copy number of a gene in a cell and does not refle...
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| Format: | Artikel |
| Sprache: | English |
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Bio-protocol LLC
2024-03-01
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| Schriftenreihe: | Bio-Protocol |
| Online Zugang: | https://bio-protocol.org/en/bpdetail?id=4960&type=0 |