Identification of novel genes for bitter taste receptors in sheep (Ovis aries)
Genetic studies on taste sensitivity, and bitter taste receptors (T2R) in particular, are an essential tool to understand ingestive behavior and its relation to variations of nutritional status occurring in ruminants. In the present study, we conducted a data-mining search to identify T2R candidates...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2013-01-01
|
| Series: | Animal |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1751731112002030 |
| _version_ | 1818864932917084160 |
|---|---|
| author | A.M. Ferreira S.S. Araújo E. Sales-Baptista A.M. Almeida |
| author_facet | A.M. Ferreira S.S. Araújo E. Sales-Baptista A.M. Almeida |
| author_sort | A.M. Ferreira |
| collection | DOAJ |
| description | Genetic studies on taste sensitivity, and bitter taste receptors (T2R) in particular, are an essential tool to understand ingestive behavior and its relation to variations of nutritional status occurring in ruminants. In the present study, we conducted a data-mining search to identify T2R candidates in sheep by comparison with the described T2R in cattle and using recently available ovine genome. In sheep, we identified eight orthologs of cattle genes: T2R16, T2R10B, T2R12, T2R3, T2R4, T2R67, T2R13 and T2R5. The in silico predicted genes were then confirmed by PCR and DNA sequencing. The sequencing results showed a 99% to 100% similarity with the in silico predicted sequence. Moreover, we address the chromosomal distribution and compare, in homology and phylogenetic terms, the obtained genes with the known T2R in human, mouse, dog, cattle, horse and pig. The eight novel genes identified map either to ovine chromosome 3 or 4. The phylogenetic data suggest a clustering by receptor type rather than by species for some of the receptors. From the species analyzed, we observed a clear proximity between the two ruminant species, sheep and cattle, in contrast with lower similarities obtained for the comparison of sheep with other mammals. Although further studies are needed to identify the complete T2R repertoire in domestic sheep, our data represent a first step for genetic studies on this field. |
| first_indexed | 2024-12-19T10:39:31Z |
| format | Article |
| id | doaj.art-7265ca2f7137480fb18ae40f7c2c36ec |
| institution | Directory Open Access Journal |
| issn | 1751-7311 |
| language | English |
| last_indexed | 2024-12-19T10:39:31Z |
| publishDate | 2013-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Animal |
| spelling | doaj.art-7265ca2f7137480fb18ae40f7c2c36ec2022-12-21T20:25:29ZengElsevierAnimal1751-73112013-01-0174547554Identification of novel genes for bitter taste receptors in sheep (Ovis aries)A.M. Ferreira0S.S. Araújo1E. Sales-Baptista2A.M. Almeida3Instituto de Ciências Agrárias e Ambientais Mediterrânicas (ICAAM), Universidade de Évora, 7000 Évora, Portugal; Plant Cell Biotechnology Laboratory, Instituto de Tecnologia Química e Biológica/Universidade Nova de Lisboa (ITQB/UNL), 2780 Oeiras, PortugalPlant Cell Biotechnology Laboratory, Instituto de Tecnologia Química e Biológica/Universidade Nova de Lisboa (ITQB/UNL), 2780 Oeiras, Portugal; Instituto de Investigação Científica Tropical (IICT) and CIISA – Centro Interdisciplinar de Investigação em Sanidade Animal, CVZ – Centro de Veterinária e Zootecnia, Faculdade de Medicina Veterinária, 1300 Lisboa, PortugalInstituto de Ciências Agrárias e Ambientais Mediterrânicas (ICAAM), Universidade de Évora, 7000 Évora, Portugal; Departamento de Zootecnia, Universidade de Évora, 7002 Évora, PortugalPlant Cell Biotechnology Laboratory, Instituto de Tecnologia Química e Biológica/Universidade Nova de Lisboa (ITQB/UNL), 2780 Oeiras, Portugal; Mass Spectrometry Laboratory, Instituto de Tecnologia Química e Biológica/Universidade Nova de Lisboa (ITQB/UNL), 2780 Oeiras, PortugalGenetic studies on taste sensitivity, and bitter taste receptors (T2R) in particular, are an essential tool to understand ingestive behavior and its relation to variations of nutritional status occurring in ruminants. In the present study, we conducted a data-mining search to identify T2R candidates in sheep by comparison with the described T2R in cattle and using recently available ovine genome. In sheep, we identified eight orthologs of cattle genes: T2R16, T2R10B, T2R12, T2R3, T2R4, T2R67, T2R13 and T2R5. The in silico predicted genes were then confirmed by PCR and DNA sequencing. The sequencing results showed a 99% to 100% similarity with the in silico predicted sequence. Moreover, we address the chromosomal distribution and compare, in homology and phylogenetic terms, the obtained genes with the known T2R in human, mouse, dog, cattle, horse and pig. The eight novel genes identified map either to ovine chromosome 3 or 4. The phylogenetic data suggest a clustering by receptor type rather than by species for some of the receptors. From the species analyzed, we observed a clear proximity between the two ruminant species, sheep and cattle, in contrast with lower similarities obtained for the comparison of sheep with other mammals. Although further studies are needed to identify the complete T2R repertoire in domestic sheep, our data represent a first step for genetic studies on this field.http://www.sciencedirect.com/science/article/pii/S1751731112002030bitter tastetaste receptorsT2Rsheepingestive behavior |
| spellingShingle | A.M. Ferreira S.S. Araújo E. Sales-Baptista A.M. Almeida Identification of novel genes for bitter taste receptors in sheep (Ovis aries) Animal bitter taste taste receptors T2R sheep ingestive behavior |
| title | Identification of novel genes for bitter taste receptors in sheep (Ovis aries) |
| title_full | Identification of novel genes for bitter taste receptors in sheep (Ovis aries) |
| title_fullStr | Identification of novel genes for bitter taste receptors in sheep (Ovis aries) |
| title_full_unstemmed | Identification of novel genes for bitter taste receptors in sheep (Ovis aries) |
| title_short | Identification of novel genes for bitter taste receptors in sheep (Ovis aries) |
| title_sort | identification of novel genes for bitter taste receptors in sheep ovis aries |
| topic | bitter taste taste receptors T2R sheep ingestive behavior |
| url | http://www.sciencedirect.com/science/article/pii/S1751731112002030 |
| work_keys_str_mv | AT amferreira identificationofnovelgenesforbittertastereceptorsinsheepovisaries AT ssaraujo identificationofnovelgenesforbittertastereceptorsinsheepovisaries AT esalesbaptista identificationofnovelgenesforbittertastereceptorsinsheepovisaries AT amalmeida identificationofnovelgenesforbittertastereceptorsinsheepovisaries |