Design of a Low-Power Radio Frequency Unit and Its Application for Bacterial Inactivation under Laboratory Conditions
A lab-scale low-power free-running radio frequency (RF) oscillator operating at a frequency of 27.12 ± 0.50 MHz was developed to be suitable for fundamental microbiological research topics. Calibration and validation were conducted for two common foodborne pathogens in relevant microbiological growt...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-11-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/11/23/11117 |
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| author | Dmytro S. Kozak Maria Tonti Patricia Cuba Julian Espitia Vladimir S. Tsepelev Davy Verheyen Simen Akkermans Jan F. M. Van Impe |
| author_facet | Dmytro S. Kozak Maria Tonti Patricia Cuba Julian Espitia Vladimir S. Tsepelev Davy Verheyen Simen Akkermans Jan F. M. Van Impe |
| author_sort | Dmytro S. Kozak |
| collection | DOAJ |
| description | A lab-scale low-power free-running radio frequency (RF) oscillator operating at a frequency of 27.12 ± 0.50 MHz was developed to be suitable for fundamental microbiological research topics. Calibration and validation were conducted for two common foodborne pathogens in relevant microbiological growth media, i.e., <i>Salmonella Typhimurium</i> and <i>Listeria monocytogenes</i> in Tryptic Soy Broth and Brain–Heart Infusion broth, respectively. The evolution of temperature, frequency, and power consumption was monitored during treatments, both with and without bacterial cells. The setup operated within the predefined frequency range, reaching temperatures of 71–76 °C after 15 min. The average power consumption ranged between 12 and 14 W. The presence of bacteria did not significantly influence the operational parameters. The inactivation potential of the RF setup was validated, demonstrating the absence of viable cells after 8 and 10 min of treatment, for <i>S. Typhimurium</i> and <i>L. monocytogenes</i>, respectively. In future studies, the setup can be used to conduct fundamental microbiological studies on RF inactivation. The setup can provide added value to the scientific field, since (i) no consensus has been reached on the inactivation mechanisms of RF inactivation of pathogens in foods and (ii) most commercial RF setups are unsuitable to adopt for fundamental studies. |
| first_indexed | 2024-03-10T04:58:11Z |
| format | Article |
| id | doaj.art-726a162897074d63a8de49181f9aff1e |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T04:58:11Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-726a162897074d63a8de49181f9aff1e2023-11-23T02:02:45ZengMDPI AGApplied Sciences2076-34172021-11-0111231111710.3390/app112311117Design of a Low-Power Radio Frequency Unit and Its Application for Bacterial Inactivation under Laboratory ConditionsDmytro S. Kozak0Maria Tonti1Patricia Cuba2Julian Espitia3Vladimir S. Tsepelev4Davy Verheyen5Simen Akkermans6Jan F. M. Van Impe7BioTeC+, Chemical and Biochemical Process Technology and Control, Department of Chemical Engineering, KU Leuven, Gebroeders de Smetstraat 1, 9000 Ghent, BelgiumBioTeC+, Chemical and Biochemical Process Technology and Control, Department of Chemical Engineering, KU Leuven, Gebroeders de Smetstraat 1, 9000 Ghent, BelgiumBioTeC+, Chemical and Biochemical Process Technology and Control, Department of Chemical Engineering, KU Leuven, Gebroeders de Smetstraat 1, 9000 Ghent, BelgiumBioTeC+, Chemical and Biochemical Process Technology and Control, Department of Chemical Engineering, KU Leuven, Gebroeders de Smetstraat 1, 9000 Ghent, BelgiumResearch Center for Metallic Liquids Physics, Ural Federal University (UrFU), 19 Mira Street, 620002 Ekaterinburg, RussiaBioTeC+, Chemical and Biochemical Process Technology and Control, Department of Chemical Engineering, KU Leuven, Gebroeders de Smetstraat 1, 9000 Ghent, BelgiumBioTeC+, Chemical and Biochemical Process Technology and Control, Department of Chemical Engineering, KU Leuven, Gebroeders de Smetstraat 1, 9000 Ghent, BelgiumBioTeC+, Chemical and Biochemical Process Technology and Control, Department of Chemical Engineering, KU Leuven, Gebroeders de Smetstraat 1, 9000 Ghent, BelgiumA lab-scale low-power free-running radio frequency (RF) oscillator operating at a frequency of 27.12 ± 0.50 MHz was developed to be suitable for fundamental microbiological research topics. Calibration and validation were conducted for two common foodborne pathogens in relevant microbiological growth media, i.e., <i>Salmonella Typhimurium</i> and <i>Listeria monocytogenes</i> in Tryptic Soy Broth and Brain–Heart Infusion broth, respectively. The evolution of temperature, frequency, and power consumption was monitored during treatments, both with and without bacterial cells. The setup operated within the predefined frequency range, reaching temperatures of 71–76 °C after 15 min. The average power consumption ranged between 12 and 14 W. The presence of bacteria did not significantly influence the operational parameters. The inactivation potential of the RF setup was validated, demonstrating the absence of viable cells after 8 and 10 min of treatment, for <i>S. Typhimurium</i> and <i>L. monocytogenes</i>, respectively. In future studies, the setup can be used to conduct fundamental microbiological studies on RF inactivation. The setup can provide added value to the scientific field, since (i) no consensus has been reached on the inactivation mechanisms of RF inactivation of pathogens in foods and (ii) most commercial RF setups are unsuitable to adopt for fundamental studies.https://www.mdpi.com/2076-3417/11/23/11117food safety<i>Listeria</i><i>Salmonella</i>dielectric heatingthermal inactivation |
| spellingShingle | Dmytro S. Kozak Maria Tonti Patricia Cuba Julian Espitia Vladimir S. Tsepelev Davy Verheyen Simen Akkermans Jan F. M. Van Impe Design of a Low-Power Radio Frequency Unit and Its Application for Bacterial Inactivation under Laboratory Conditions Applied Sciences food safety <i>Listeria</i> <i>Salmonella</i> dielectric heating thermal inactivation |
| title | Design of a Low-Power Radio Frequency Unit and Its Application for Bacterial Inactivation under Laboratory Conditions |
| title_full | Design of a Low-Power Radio Frequency Unit and Its Application for Bacterial Inactivation under Laboratory Conditions |
| title_fullStr | Design of a Low-Power Radio Frequency Unit and Its Application for Bacterial Inactivation under Laboratory Conditions |
| title_full_unstemmed | Design of a Low-Power Radio Frequency Unit and Its Application for Bacterial Inactivation under Laboratory Conditions |
| title_short | Design of a Low-Power Radio Frequency Unit and Its Application for Bacterial Inactivation under Laboratory Conditions |
| title_sort | design of a low power radio frequency unit and its application for bacterial inactivation under laboratory conditions |
| topic | food safety <i>Listeria</i> <i>Salmonella</i> dielectric heating thermal inactivation |
| url | https://www.mdpi.com/2076-3417/11/23/11117 |
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