Evaluation of protective efficacy of respiratory syncytial virus vaccine against A and B subgroup human isolates in Korea.
Human respiratory syncytial virus (HRSV) is a significant cause of upper and lower respiratory tract illness mainly in infants and young children worldwide. HRSV is divided into two subgroups, HRSV-A and HRSV-B, based on sequence variation within the G gene. Despite its importance as a respiratory p...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3168431?pdf=render |
| _version_ | 1819022067738083328 |
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| author | Ji-Eun Jang Jee-Boong Lee Kyung-Hyo Kim Sung Moo Park Byoung-Shik Shim In Soo Cheon Man Ki Song Jun Chang |
| author_facet | Ji-Eun Jang Jee-Boong Lee Kyung-Hyo Kim Sung Moo Park Byoung-Shik Shim In Soo Cheon Man Ki Song Jun Chang |
| author_sort | Ji-Eun Jang |
| collection | DOAJ |
| description | Human respiratory syncytial virus (HRSV) is a significant cause of upper and lower respiratory tract illness mainly in infants and young children worldwide. HRSV is divided into two subgroups, HRSV-A and HRSV-B, based on sequence variation within the G gene. Despite its importance as a respiratory pathogen, there is currently no safe and effective vaccine for HRSV. In this study, we have detected and identified the HRSV by RT-PCR from nasopharyngeal aspirates of Korean pediatric patients. Interestingly, all HRSV-B isolates exhibited unique deletion of 6 nucleotides and duplication of 60 nucleotides in the G gene. We successfully amplified two isolates ('KR/A/09-8' belonging to HRSV-A and 'KR/B/10-12' to HRSV-B) on large-scale, and evaluated the cross-protective efficacy of our recombinant adenovirus-based HRSV vaccine candidate, rAd/3xG, by challenging the immunized mice with these isolates. The single intranasal immunization with rAd/3xG protected the mice completely from KR/A/09-8 infection and partially from KR/B/10-12 infection. Our study contributes to the understanding of the genetic characteristics and distribution of subgroups in the seasonal HRSV epidemics in Korea and, for the first time, to the evaluation of the cross-protective efficacy of RSV vaccine against HRSV-A and -B field-isolates. |
| first_indexed | 2024-12-21T04:17:06Z |
| format | Article |
| id | doaj.art-726a7e69c93c46559adbc13683f5ecfe |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T04:17:06Z |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-726a7e69c93c46559adbc13683f5ecfe2022-12-21T19:16:17ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0169e2379710.1371/journal.pone.0023797Evaluation of protective efficacy of respiratory syncytial virus vaccine against A and B subgroup human isolates in Korea.Ji-Eun JangJee-Boong LeeKyung-Hyo KimSung Moo ParkByoung-Shik ShimIn Soo CheonMan Ki SongJun ChangHuman respiratory syncytial virus (HRSV) is a significant cause of upper and lower respiratory tract illness mainly in infants and young children worldwide. HRSV is divided into two subgroups, HRSV-A and HRSV-B, based on sequence variation within the G gene. Despite its importance as a respiratory pathogen, there is currently no safe and effective vaccine for HRSV. In this study, we have detected and identified the HRSV by RT-PCR from nasopharyngeal aspirates of Korean pediatric patients. Interestingly, all HRSV-B isolates exhibited unique deletion of 6 nucleotides and duplication of 60 nucleotides in the G gene. We successfully amplified two isolates ('KR/A/09-8' belonging to HRSV-A and 'KR/B/10-12' to HRSV-B) on large-scale, and evaluated the cross-protective efficacy of our recombinant adenovirus-based HRSV vaccine candidate, rAd/3xG, by challenging the immunized mice with these isolates. The single intranasal immunization with rAd/3xG protected the mice completely from KR/A/09-8 infection and partially from KR/B/10-12 infection. Our study contributes to the understanding of the genetic characteristics and distribution of subgroups in the seasonal HRSV epidemics in Korea and, for the first time, to the evaluation of the cross-protective efficacy of RSV vaccine against HRSV-A and -B field-isolates.http://europepmc.org/articles/PMC3168431?pdf=render |
| spellingShingle | Ji-Eun Jang Jee-Boong Lee Kyung-Hyo Kim Sung Moo Park Byoung-Shik Shim In Soo Cheon Man Ki Song Jun Chang Evaluation of protective efficacy of respiratory syncytial virus vaccine against A and B subgroup human isolates in Korea. PLoS ONE |
| title | Evaluation of protective efficacy of respiratory syncytial virus vaccine against A and B subgroup human isolates in Korea. |
| title_full | Evaluation of protective efficacy of respiratory syncytial virus vaccine against A and B subgroup human isolates in Korea. |
| title_fullStr | Evaluation of protective efficacy of respiratory syncytial virus vaccine against A and B subgroup human isolates in Korea. |
| title_full_unstemmed | Evaluation of protective efficacy of respiratory syncytial virus vaccine against A and B subgroup human isolates in Korea. |
| title_short | Evaluation of protective efficacy of respiratory syncytial virus vaccine against A and B subgroup human isolates in Korea. |
| title_sort | evaluation of protective efficacy of respiratory syncytial virus vaccine against a and b subgroup human isolates in korea |
| url | http://europepmc.org/articles/PMC3168431?pdf=render |
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