PEG Gels Significantly Improve the Storage Stability of Nucleic Acid Preparations
Currently, nucleic acid preparations have gained much attention due to their unique working principle and application value. However, as macromolecular drugs, nucleic acid preparations have complex construction and poor stability. The current methods to promote stability face problems such as high c...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-12-01
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| Series: | Gels |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2310-2861/8/12/819 |
| _version_ | 1797458044515254272 |
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| author | Pengfei Cui Luping Ma Pengju Jiang Cheng Wang Jianhao Wang |
| author_facet | Pengfei Cui Luping Ma Pengju Jiang Cheng Wang Jianhao Wang |
| author_sort | Pengfei Cui |
| collection | DOAJ |
| description | Currently, nucleic acid preparations have gained much attention due to their unique working principle and application value. However, as macromolecular drugs, nucleic acid preparations have complex construction and poor stability. The current methods to promote stability face problems such as high cost and inconvenient operatios. In this study, the hydrophilic pharmaceutical excipient PEG was used to gelate nucleic acid preparations to avoid the random movements of liquid particles. The results showed that PEG gelation significantly improved the stability of PEI25K−based and liposome−based nucleic acid preparations, compared with nucleic acid preparations without PEG gelation. After being stored at 4 °C for 3 days, non−PEG gelled nucleic acid preparations almost lost transfection activity, while PEGylated preparations still maintained high transfection efficiency. Fluorescence experiments showed that this effect was caused by inhibiting particle aggregation. The method described in this study was simple and effective, and the materials used had good biocompatibility. It is believed that this study will contribute to the better development of gene therapy drugs. |
| first_indexed | 2024-03-09T16:31:27Z |
| format | Article |
| id | doaj.art-726eb618b6314fe2b692829dc4702b68 |
| institution | Directory Open Access Journal |
| issn | 2310-2861 |
| language | English |
| last_indexed | 2024-03-09T16:31:27Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Gels |
| spelling | doaj.art-726eb618b6314fe2b692829dc4702b682023-11-24T15:01:37ZengMDPI AGGels2310-28612022-12-0181281910.3390/gels8120819PEG Gels Significantly Improve the Storage Stability of Nucleic Acid PreparationsPengfei Cui0Luping Ma1Pengju Jiang2Cheng Wang3Jianhao Wang4School of Pharmacy, Changzhou University, Changzhou 213164, ChinaSchool of Pharmacy, Changzhou University, Changzhou 213164, ChinaSchool of Pharmacy, Changzhou University, Changzhou 213164, ChinaSchool of Pharmacy, Changzhou University, Changzhou 213164, ChinaSchool of Pharmacy, Changzhou University, Changzhou 213164, ChinaCurrently, nucleic acid preparations have gained much attention due to their unique working principle and application value. However, as macromolecular drugs, nucleic acid preparations have complex construction and poor stability. The current methods to promote stability face problems such as high cost and inconvenient operatios. In this study, the hydrophilic pharmaceutical excipient PEG was used to gelate nucleic acid preparations to avoid the random movements of liquid particles. The results showed that PEG gelation significantly improved the stability of PEI25K−based and liposome−based nucleic acid preparations, compared with nucleic acid preparations without PEG gelation. After being stored at 4 °C for 3 days, non−PEG gelled nucleic acid preparations almost lost transfection activity, while PEGylated preparations still maintained high transfection efficiency. Fluorescence experiments showed that this effect was caused by inhibiting particle aggregation. The method described in this study was simple and effective, and the materials used had good biocompatibility. It is believed that this study will contribute to the better development of gene therapy drugs.https://www.mdpi.com/2310-2861/8/12/819nucleic acid preparationPEG gelationstability |
| spellingShingle | Pengfei Cui Luping Ma Pengju Jiang Cheng Wang Jianhao Wang PEG Gels Significantly Improve the Storage Stability of Nucleic Acid Preparations Gels nucleic acid preparation PEG gelation stability |
| title | PEG Gels Significantly Improve the Storage Stability of Nucleic Acid Preparations |
| title_full | PEG Gels Significantly Improve the Storage Stability of Nucleic Acid Preparations |
| title_fullStr | PEG Gels Significantly Improve the Storage Stability of Nucleic Acid Preparations |
| title_full_unstemmed | PEG Gels Significantly Improve the Storage Stability of Nucleic Acid Preparations |
| title_short | PEG Gels Significantly Improve the Storage Stability of Nucleic Acid Preparations |
| title_sort | peg gels significantly improve the storage stability of nucleic acid preparations |
| topic | nucleic acid preparation PEG gelation stability |
| url | https://www.mdpi.com/2310-2861/8/12/819 |
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